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InvisibleDoctor Mario
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Re: Trying isolation with agar and petri dishes - thoughts? [Re: WunFunGai]
    #26946608 - 09/20/20 05:51 PM (3 years, 4 months ago)

Hell yea! Good job, bro.


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OfflineWunFunGai
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Re: Trying isolation with agar and petri dishes - thoughts? [Re: Doctor Mario]
    #26947087 - 09/21/20 12:02 AM (3 years, 4 months ago)

<-- Thanks! Things still moving along...

NOTE: I've also posted similar info in the daily cult pic of the day thread as well, but wanted these here as part of the overall log.

Update: The tub with the most progress already has some very small fruits that have broken their veils. They are pretty small and skinny though. Not sure if that's just genetics or something about how I've set up.

I'm curious if it is normal to sometimes initially have some small fruits like this or if it's a sign of how the rest of the tub will be.

I'm pretty happy with what I've learned with my first time trying plates and taking spores to agar, transferring and going to grain jars. I've got another set of jars I'm spawning that are off the second transfer and am hopeful they will be good.

Three 6qt shoebox tubs running from 1st xfer:


Close ups: these guys are really small but look normal otherwise.


Conditions inside look pretty good, condensation on sides, microbeads on surface still. Not fussing with anything and letting them run for now.

Contemplating putting a flipped tub on top either with or without holes to give them some headroom.

Enjoy.

WFG :smile:


--------------------
The Official TNF Reccomended Teks & Methods
Bod's Simplified Cultivation Methods <-- Awesome!!!
D3monics Perfect Transfers and Agar Tek

No matter where you go, there you are... - Buckaroo Banzai

You gotta be here, if you're not all there...


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InvisibleDoctor Mario
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Re: Trying isolation with agar and petri dishes - thoughts? [Re: WunFunGai]
    #26947435 - 09/21/20 08:56 AM (3 years, 4 months ago)

I believe its common to have smaller sized fruits in shoeboxes. Less substrate means less water. If mushrooms have a large supply of water, they can get much bigger. It doesn't nessecarily mean that you need to use a bigger tub but it would be harder to keep a sub that size fully hydrated without constantly baby sitting and accidentally over watering.

It could also be genetics. Definitely just let them do their thing. Often times, people end up with a higher total weight from the small guys vs a tub with some bigger ones and usually get bigger fruit from the second flush.

Either way, I'm stoked for you! Thanks for continuing to document this for us.


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Offlineseand04
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Re: Trying isolation with agar and petri dishes - thoughts? [Re: Doctor Mario]
    #26947522 - 09/21/20 10:14 AM (3 years, 4 months ago)

Golden plates for golden halo spores.


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Offlineverum subsequentis
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Re: Trying isolation with agar and petri dishes - thoughts? [Re: seand04]
    #26947568 - 09/21/20 10:39 AM (3 years, 4 months ago)

:popcorn:


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OfflineWunFunGai
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Re: Trying isolation with agar and petri dishes - thoughts? [Re: verum subsequentis]
    #26948724 - 09/22/20 01:16 AM (3 years, 4 months ago)

Update 09/21: Alrighty then...got a response on previous pics and requests for input posted in the Post Your Cultivation Pics thread that were helpful in pointing out that my lil buddies may be searching for more air by the way they were searching for gaps in tub.

Based on that decided to give them some more headroom and air, and threw some old tubs I had with holes in them from my early mini-dub tub projects.

This has always been a funky part of the path for me in correctly observing conditions and doing the right things to keep them ideal.

Any veteran/TC observations or suggestions are welcome, but I keep telling myself my lil buddies know what to do and trying to trust them to do it. LOL

Rather than oscillate wildly changing extremes by fanning or misting like a crazed monkey on crack, I'm trying to just hang back and watch as things develop and only make changes when obviously necessary instead of fretting over it...don't get me wrong..I'm still fretting over it. I'm just letting it go...



Good Kids & The New Kids On The Block (sorry, couldn't resist): Kids seem to be doing OK so far with one day of extra headroom and surface conditions holding. Spawned the New Kids from second transfer and they were a bit more robust in colonizing jars, although I think relative time is similar. One tub missing to the right, but they were just spawned...Nothing to see here, move along....:wink:



Glamour shots of the Good Kids: All three tubs seem to be OK, condensation seems to be a lot less on the sides so I'm a bit worried about if they'll dry out, but at the moment surface conditions show microbeads and I don't see anything with my not so trained eye that looks wrong. Anyone see anything I'm missing or might suggest that I either watch or adjust for? Average ~80-84F / 55%-65% Humidity daily.

As always, comments/observations/questions, suggestions welcome.

Enjoy.

WFG :smile:


--------------------
The Official TNF Reccomended Teks & Methods
Bod's Simplified Cultivation Methods <-- Awesome!!!
D3monics Perfect Transfers and Agar Tek

No matter where you go, there you are... - Buckaroo Banzai

You gotta be here, if you're not all there...


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OfflineWunFunGai
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Registered: 05/03/16
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Re: Trying isolation with agar and petri dishes - thoughts? [Re: WunFunGai]
    #26950873 - 09/23/20 10:49 AM (3 years, 4 months ago)

Update 09/22: First Fruits...the highly awaited moment (for me at least) was getting to first fruit and it happened. No canopy or monster flush so a bit anti-climatic. :undecided:

It's been a fun ride so far, relearning some old things, and taking some chances with doing some new things, and adjusting and learning along the way.

I've got a second set of tubs running that are from second transfer that appear to be much growing much more aggressively - Strands were sticking out of sub already yesterday afternoon. Would be nice to see a canopy or bigger fruits, but I hope I have a fairly decently logged a cycle from spore->agar->clean->agar->grain->tub->fruits.


First Fruit 09/22: Kinda small first pull, but they were splooging spores already so I pulled them. Enough for a "test run" this weekend. Wet 22.0g / Dry 2.7g.


Tubs 09/22: Tubs are moving along. Surface conditions holding up, haven't touched or done anything other than putting old shoeboxes with holes on top to give headspace, and a peek and light fanning in morning and evening. The fanning isn't for any particular purpose other than it makes the immediate area smell good...lol

Thanks, if you've stuck around for the ride and reading the tale of the journey so far. :thanx:

If you've offered advice or encouragement, BIG thanks! That is what makes it worth taking time to look at my own process and how I'm doing things, how pictures are taken, retaken, and cleaned up, and helped me understand what I'm doing better...even if you can't offer advice. Knowing there's folks who are possibly looking at what they do in a new or different light is worth the effort. :bow2:

It would have been cool to have gotten a full canopy or monster first flush, but this roll of the dice didn't turn it up. But I do have a pretty solid process to continue transferring from what I have, or to start a new cycle and roll of the genetic dice.

It's piqued my interest in trying other varieties or even trying to acquire spores from a strain that has unique/proven characteristics to see if I can turn out similar results. I'm actually a bit excited about all these little myco rabbit holes to explore and learn from with what I gained from this little project.

And now that I turn around and look back to the start, this thread may not really have an appropriate title. It will take me some more transfers and research to get to "isolating". As I learned through this, and read more, I've only really cleaned up spores to agar and taken them to a SFF tub.

Not sure what I'd have called it now that I know what I know, but wondering if changing it worth doing if it might help others understand the context of this thread. Any suggestions on a better title?

Not sure if there's any interest to continue logging the progress of the second transfer tubs or if I should start considering a new project.

If you enjoyed or got something out of this thread, comments or suggestions welcome that I can use to improve the next log I post. Or throw a rate my way if it's worthy.. :smile:

Enjoy.

WFG :smile:


--------------------
The Official TNF Reccomended Teks & Methods
Bod's Simplified Cultivation Methods <-- Awesome!!!
D3monics Perfect Transfers and Agar Tek

No matter where you go, there you are... - Buckaroo Banzai

You gotta be here, if you're not all there...


Edited by WunFunGai (09/23/20 12:30 PM)


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InvisibleDoctor Mario
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Re: Trying isolation with agar and petri dishes - thoughts? [Re: WunFunGai]
    #26950999 - 09/23/20 12:31 PM (3 years, 4 months ago)

Looking great, man! Don't be sad about not seeing a canopy. Clone a piece from one of those clusters and the next tub might just be a canopy, if not a bunch of clusters.


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