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wonderousovoid
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Possible contam identification
#26948625 - 09/21/20 11:05 PM (3 years, 4 months ago) |
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Jar 1 for sure contam but I'm wondering what it may be?
 Jar 2, the agar puck was facing inwards(like the mycelium side not facing the glass) so I think the myc is just growing wierd as it grows out but there's a few spots that kinda look like the first jars growth. Near 7-9ish o'clock area
Edited by wonderousovoid (09/21/20 11:06 PM)
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tripdawg420
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looks like a few grains touched the wedge and started growing
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wonderousovoid
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Re: Possible contam identification [Re: tripdawg420]
#26948931 - 09/22/20 08:15 AM (3 years, 4 months ago) |
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Which jar are you referring to?
Anyone know what might be the contamination in the first set of pics?
The second jar I'm fairly certain is okay and just growing odd since the myc was facing the inside of the jar and now its starting to grow out
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Josex
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Quote:
wonderousovoid said: Anyone know what might be the contamination in the first set of pics?
It's probably some type of pin mold, but why would you need to know what type of mold it is? All you need to know is that it's mold and you have to trash it ASAP. A crap is still a crap no matter the color and you flush it down the toilet without a second thought, right?
I'd be more interested in knowing why and how it got there. In this case it's pretty obvious it got there during inoculation. Maybe knock up your jars with wedges next time and quit the "Tiger drop". I don't think you'll see lots of experienced cultivators dropping a whole puck in a jar, shit's an eyesore and sloppy as fuck.
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Pretendhole
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Re: Possible contam identification [Re: Josex]
#26948956 - 09/22/20 08:42 AM (3 years, 4 months ago) |
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Trichoderma harzianum
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wonderousovoid
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Re: Possible contam identification [Re: Josex]
#26948979 - 09/22/20 09:03 AM (3 years, 4 months ago) |
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Quote:
Josex said:
Quote:
wonderousovoid said: Anyone know what might be the contamination in the first set of pics?
It's probably some type of pin mold, but why would you need to know what type of mold it is? All you need to know is that it's mold and you have to trash it ASAP. A crap is still a crap no matter the color and you flush it down the toilet without a second thought, right?
I'd be more interested in knowing why and how it got there. In this case it's pretty obvious it got there during inoculation. Maybe knock up your jars with wedges next time and quit the "Tiger drop". I don't think you'll see lots of experienced cultivators dropping a whole puck in a jar, shit's an eyesore and sloppy as fuck.
What a condescending and useless post. The only thing I got out of your response is that it may be a pin mold. The rest just makes you look like a prick in reality. I wasn't asking for any advise on my technique or if I should trash these or not.
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wonderousovoid
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Re: Possible contam identification [Re: Pretendhole]
#26948987 - 09/22/20 09:12 AM (3 years, 4 months ago) |
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Quote:
Pretendhole said: Trichoderma harzianum
Thank you! Qaulity post
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Josex
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Lol that's not trich. Condescending and useless is in the eyes of the beholder. I was being matter of fact. It's not useless to tell you to focus on the important thing here (technique) by telling you to inoculate with wedges vs a whole puck, as I'd be willling to bet the ranch you probably wouldn't have seen mold if you'd have used a wedge instead. It's a much safer procedure, period. But you keep doing you if you think my post was useless. 
You're growing drugs, grow a thicker skin.
Edited by Josex (09/22/20 10:13 AM)
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wonderousovoid
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Re: Possible contam identification [Re: Josex]
#26949089 - 09/22/20 10:26 AM (3 years, 4 months ago) |
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This guy might have the biggest brain on the website. Why don't you have a tc tag yet?
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Josex
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Cry some more.
Keep on focusing on how the info was delivered to you instead of trying to take something from it and you'll go far. That's how mopey bitches grow shrooms.
The only useless thing here was the question you asked. Wtf does it matter to you the name of the mold you have there? It's for a school project or something? You'd be better off knowing where you fucked up so you can fix it. Sorry for not sugar coating my post.
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wonderousovoid
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Re: Possible contam identification [Re: Josex]
#26949138 - 09/22/20 11:04 AM (3 years, 4 months ago) |
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King shit of turd island showed up and won't stop arguing with himself
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mushboy
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That's definitely pin mold of sorts. Looks like it was either hiding in your culture or was introduced during inoculation.
I havent used a whole puck in ages. Next time do a wedge. I can almost guarantee you'll have similar colonizing times without the hassle of tiger dropping.
Second jar is fine looking. Sometimes you'll notice satellite myc colonies when working with buried wedges/pucks. Even a microscopic fragment of myc is enough to colonize an entire jar(hence the poke method from your new best friend)
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Pastywhyte
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I find tiger drop to be perfectly safe it’s no different than wedges other than it has fewer movements and no actual contact is made to the colony. I mean if you bounce it off the rim and then spear it up off the floor and jam it in that’s no good. But a properly performed drop is no different than G2G or a wedge to grain. It also colonizes far faster than a small ass wedge will.
I do the tic tac toe version instead now, it’s super easy and huge inoculation potential. Sometimes I don’t even need to shake.
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tripdawg420
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Re: Possible contam identification [Re: Pastywhyte]
#26949506 - 09/22/20 02:45 PM (3 years, 4 months ago) |
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not needing to shake damn i need that genetics
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mushboy
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Re: Possible contam identification [Re: Pastywhyte]
#26949517 - 09/22/20 02:50 PM (3 years, 4 months ago) |
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I didnt like dropping because I felt the hand motions from squeezing the upside down container directly above open sterile media to be difficult without something undesirable falling into said jar from my hand motions.
I now do a wedge with a barely cracked open lid and using the rim of the jar to 'pull' the wedge off the blade. Limiting the area of exposed media(open jar) and immediately closing back up imo is safer.
You either grow the colony on the wedge and drop the whole thing or drop a smaller wedge early and grow the colony on the grains.
Edited by mushboy (09/22/20 02:52 PM)
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tryptkaloids
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Re: Possible contam identification [Re: Pastywhyte]
#26949518 - 09/22/20 02:50 PM (3 years, 4 months ago) |
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Im inclined to agree with pasty here.
That being said, its clear as day to me that mold was on the agar before the drop
-------------------- "Remember, kids, the difference between science and screwing around is writing it down" -adam savage Flowchart for Recommended plan of action. Learn the tried and true way to grow mushrooms Use the Damn search engine After you know what you're doing, take a break Pick a book, Make some chips! Josex said:Don't take the site seriously bro, ain't worth it.
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tryptkaloids
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Re: Possible contam identification [Re: mushboy]
#26949521 - 09/22/20 02:51 PM (3 years, 4 months ago) |
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Quote:
mushboy said: I didnt like dropping because I felt the hand motions from squeezing the upside down container directly above open sterile media to be difficult without something undesirable falling into said jar from my hand motions.
I now do a wedge with a barely cracked open lid and using the rim of the jar to 'pull' the wedge off the blade. Limiting the area of exposed media(open jar) and immediately closing back up imo is safer.
I loosen the puck before I open anything
-------------------- "Remember, kids, the difference between science and screwing around is writing it down" -adam savage Flowchart for Recommended plan of action. Learn the tried and true way to grow mushrooms Use the Damn search engine After you know what you're doing, take a break Pick a book, Make some chips! Josex said:Don't take the site seriously bro, ain't worth it.
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tripdawg420
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Re: Possible contam identification [Re: tryptkaloids]
#26949525 - 09/22/20 02:52 PM (3 years, 4 months ago) |
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on the second set of pics i dont see anything
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Josex
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Re: Possible contam identification [Re: tripdawg420]
#26949746 - 09/22/20 04:48 PM (3 years, 4 months ago) |
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I agree that in principle the tiger drop should not be riskier than pouring or g2g'ing. I actually did it a lot in a SAB back when I was still using glad mini rounds for agar with great success. However, the motions I had to perform to drop the puck never felt quite as precise or safe as inoculating with regular wedges, doing exactly what Mushboy was describing, so I quickly got back to just dropping wedges because if I can choose between 2 ways of doing things I go with the one that feels safer.
There's also a much higher chance of existing hidden contams in a whole puck vs a wedge, and you can also knock up a bunch of jars with a single agar puck if you cut wedges. Dropping the whole puck is not something I'd recommend to a beginner but it's perfectly fine if you already know what you're doing and feel comfortable with it.
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Pastywhyte
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Re: Possible contam identification [Re: Josex]
#26949754 - 09/22/20 04:56 PM (3 years, 4 months ago) |
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If I wasn’t using LC for everything right now I would be definitely dropping chopped up pucks. You loosen it all well before you drop them and just do it like a G2G. I personally think noobs should also try G2G. People shouldn’t sell their potential short
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