|
ataribravo
Magic Myc

Registered: 08/07/20
Posts: 75
Last seen: 2 years, 10 months
|
Isopropyl alcohol - Does it or doesn't it??
#26948272 - 09/21/20 06:18 PM (3 years, 4 months ago) |
|
|
I have read many posts on here (and had responses to posts I have made) that say ISO does NOT sterilize, it only sanitizes. I have been beating my head against a brick wall trying to figure out how to sterilize something that can neither be pressure cooked or flamed (e.g. plastics).
I looked up "does Isopropyl alcohol sterilize" and found many results that claim it does and is used for surgical equipment and operating rooms in hospitals as an example. I even found a dissertation claiming that is does sterilize.
We all use it to clean surfaces, spray our gloves, SABs, jars and so on. I know the difference between sanitize and sterilize. Who here says ISO sterilizes and who says it only sanitizes, and why?
|
RoscoeReturns
Crotchety chode man



Registered: 02/12/18
Posts: 1,738
Loc: State of Confusion
Last seen: 4 hours, 50 minutes
|
Re: Isopropyl alcohol - Does it or doesn't it?? [Re: ataribravo]
#26948287 - 09/21/20 06:30 PM (3 years, 4 months ago) |
|
|
It does not. It sanitizes. Most ISO you buy isn’t even sterile. We have to buy sterile ISO (quite a bit more expensive), or filter regular ISO before we use it.
Edit: I use sterile ISO at work, not for mush cult.
Plastics can be sterilized by gas, or gamma irradiation. Not really an option at home. What are you trying to sterilize?
Edited by RoscoeReturns (09/21/20 06:32 PM)
|
ataribravo
Magic Myc

Registered: 08/07/20
Posts: 75
Last seen: 2 years, 10 months
|
Re: Isopropyl alcohol - Does it or doesn't it?? [Re: RoscoeReturns]
#26948327 - 09/21/20 07:01 PM (3 years, 4 months ago) |
|
|
Quote:
RoscoeReturns said: It does not. It sanitizes. Most ISO you buy isn’t even sterile. We have to buy sterile ISO (quite a bit more expensive), or filter regular ISO before we use it.
Edit: I use sterile ISO at work, not for mush cult.
Plastics can be sterilized by gas, or gamma irradiation. Not really an option at home. What are you trying to sterilize?
I am trying to use blended LI using distilled water and agar plates. The container for the blender (NurtiBullet) is plastic. Also, if there was a way, plastic petri dishes, but that's not so important. I bought a 60W UVC light in hopes of decontaminating my shroom room, but I guess that has little value due to minimal penetration. Would UVC work on spores on surfaces though? I think I'm just going to go blender-less and use PC'd jars.
Ever heard of Spor-Klenz? Claims to be a spore sterilant using Peracetic acid, hydrogen perozide and acetic acid.
Edited by ataribravo (09/21/20 07:05 PM)
|
travels_slightly
Tryptémon/Phenethylémon trainer


Registered: 07/04/20
Posts: 47
Loc: The central California Co...
Last seen: 3 years, 2 months
|
Re: Isopropyl alcohol - Does it or doesn't it?? [Re: RoscoeReturns]
#26948342 - 09/21/20 07:06 PM (3 years, 4 months ago) |
|
|
Noting the difference between standard isopropyl alcohol that you get at any store and true isopropanol that you have to order from chem suppliers, but does that mean that the latter is truly sterilizing?
-------------------- General supporter of all things mystifying, specially when they happen to simultaneously be incriminating. ISO spores beyond my limited collection. I make and deal in many wares, perhaps we can exchange cool goodies
|
Gan
Wielder of Narya



Registered: 08/26/19
Posts: 927
Loc: Valinor
Last seen: 5 months, 27 days
|
|
No... neither is sterilizing. Iso on it's own is incapable of sterilizing. Only sanitizing is possible.
|
Sir Pentinite
Stranger all the time.

Registered: 05/15/19
Posts: 525
Loc: ation Location Location
|
|
I think the fact that you can get sterile iso and non-sterile iso answers the question. Consumer-grade iso is not sterile and isopropanol in and of itself does not sterilize. It doesn't reliably kill endospores for one thing.
Quote:
travels_slightly said: Noting the difference between standard isopropyl alcohol that you get at any store and true isopropanol that you have to order from chem suppliers, but does that mean that the latter is truly sterilizing?
No, it's the same compound. The biggest differences between Wally World iso and the stuff from a lab supply is the lab-grade has traceabilty through lot numbers and usually a certificate of analysis. Even then it's not sterile unless you specify it and it's only sterile until you open it.
-------------------- "I thought to myself 'Boy, I'm sure glad there's nobody here to see this because this is exactly the sort of thing that gets people riled-up and they assume you're dying and that something has to be done. Where if you're alone, you know, you either come through it or you die, but in any case you avoid the fuss.'" - Terrence McKenna
|
RoscoeReturns
Crotchety chode man



Registered: 02/12/18
Posts: 1,738
Loc: State of Confusion
Last seen: 4 hours, 50 minutes
|
Re: Isopropyl alcohol - Does it or doesn't it?? [Re: ataribravo]
#26948844 - 09/22/20 06:22 AM (3 years, 4 months ago) |
|
|
Quote:
ataribravo said: I am trying to use blended LI using distilled water and agar plates. The container for the blender (NurtiBullet) is plastic. Also, if there was a way, plastic petri dishes, but that's not so important. I bought a 60W UVC light in hopes of decontaminating my shroom room, but I guess that has little value due to minimal penetration. Would UVC work on spores on surfaces though? I think I'm just going to go blender-less and use PC'd jars.
I would do blenderless, or get yourself a $25 Oster classic blender. The blade comes off the pitcher and is an exact fit on regular mouth Mason jars. I used this set up for a bit. Pint jars with the ring, Oster blade and gasket, half full with water. It can go through the PC. After it’s cooled and you drop an agar wedge in it, you pop it on the blender base and go.
Quote:
ataribravo said: Ever heard of Spor-Klenz? Claims to be a spore sterilant using Peracetic acid, hydrogen perozide and acetic acid.
I have. Still only a sanitizer as a topical surface preparation. It is better at killing some spores, specifically C. difficile spores, which is very important in a hospital setting which is what it’s marketed for. Also in places that sanitize regularly, it’s good to switch up agents used to prevent selecting for resistant organisms. We switch between a dilute bleach solution, peracetic acid, and a quaternary ammonia product.
Vaporized hydrogen peroxide can be used to sterilize, but it’s dangerous and very hard on the environment it’s being used in. Also not talking drug store H2O2, but 20% or higher. Nasty stuff.
|
|
|
You cannot start new topics / You cannot reply to topics HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a 311 topic views. 34 members, 221 guests and 47 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ] |
|