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Offlineverum subsequentis
seeker of truth
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Registered: 03/22/16
Posts: 8,732
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Re: Filtration when PC'ing bags [Re: meowjinx]
    #26946315 - 09/20/20 02:37 PM (3 years, 4 months ago)

Always a good plan when facing catastrophic loss of spawn. Sometimes it pays to hit reset, take the game out and blow on it and then try anew.


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InvisibleThomsen
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Registered: 09/27/19
Posts: 364
Re: Filtration when PC'ing bags [Re: verum subsequentis]
    #26946400 - 09/20/20 03:31 PM (3 years, 4 months ago)

No grain NTC yet. I wanted to test out the filtration first to see if that made any difference. Untill now I've only testet out my aseptic technique which is fine.


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Offlinemeowjinx
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Re: Filtration when PC'ing bags [Re: Thomsen]
    #26946471 - 09/20/20 04:12 PM (3 years, 4 months ago)

Well the bacteria has to come from somewhere, and there's only so many places that could be. You could also consider trying antibiotic agar as another way of implementing a control

You could do multiple runs of each control just tobe "statistically" sure of which step it is. Once you find what's causing it it's just a matter of fixing it. Bada bing, bada boom. No more bacteria


Edited by meowjinx (09/20/20 04:14 PM)


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InvisibleThomsen
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Registered: 09/27/19
Posts: 364
Re: Filtration when PC'ing bags [Re: meowjinx]
    #26947158 - 09/21/20 02:58 AM (3 years, 4 months ago)

Yeah antibiotic agar is never going to happen. No one should ever do that when growing mushrooms.

And yes, there's only so many places contam can come from, that's why I'm ruling out different things one at the time.


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Offlineverum subsequentis
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Registered: 03/22/16
Posts: 8,732
Last seen: 1 year, 8 months
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Re: Filtration when PC'ing bags [Re: Thomsen]
    #26947502 - 09/21/20 10:03 AM (3 years, 4 months ago)

A lot of folks use antibiotic agar. I have what it takes but don't really ever bust it out. I would if i had a culture that i really wanted to save but couldn't seem to get clean.


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