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Offlinedfittlesticks
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Registered: 05/14/20
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Inoculating one grain bag with another?
    #26947470 - 09/21/20 09:38 AM (3 years, 4 months ago)

So I inoculated 2 grain bags about 20 days ago.  One bag is showing mycelium growth of around 15% on the surface (probably more internally), however the other bag is completely growth free.  The syringe I inoculated with had been in my refrigerator for a month or so, so I am thinking it probably wasn't the best live spores.  I am thinking of taking a chunk of mycelium out of the one bag and transplanting it into the other grain bag, but I don't want to risk contamination. 

My question is, if I am going to transplant should I do it now, or maybe wait until the growth is closer to 100% in the one bag as it will be less vulnerable to contamination.  Has anybody ever done this?


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Invisiblemaxmush
Always learning...

Registered: 06/13/20
Posts: 440
Re: Inoculating one grain bag with another? [Re: dfittlesticks]
    #26947498 - 09/21/20 09:56 AM (3 years, 4 months ago)

Standard disclosure - spore syringe to grain not recommended.

If you are going to try grain to grain, then wait until the first bag is 100% and do it under a still air box.


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Offlineverum subsequentis
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Registered: 03/22/16
Posts: 8,732
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Re: Inoculating one grain bag with another? [Re: maxmush]
    #26947515 - 09/21/20 10:09 AM (3 years, 4 months ago)

I've done it many times. It's doable but not for beginners. As stated above, it's called a G2G (grain to grain). If you don't know what that is then the likely hood of you pulling it off successfully is slim. Search the site and read a little. All the info is out there.

It really wouldn't matter if you did it now or waited for more growth. If you do everything correctly (it's difficult with bags) you'll move some colonized grain from one bag to another via gravity (or maybe a heat sterilized tool) and then seal them back up without introducing any poop into either bag.

Conventional wisdom says to wait until full colonization and there are reasons for that. But that same wisdom says don't put vendor spores to vendor grain bags and there's also reason for that.


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InvisibleMunchauzen
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Registered: 06/22/11
Posts: 14,342
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Re: Inoculating one grain bag with another? [Re: verum subsequentis]
    #26947642 - 09/21/20 11:23 AM (3 years, 4 months ago)

A syringe is supposed to be stored in the fridge and is viable for many months, if not years.


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