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OfflineSurfingmycelium
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Re: Liquid culture [Re: The Fresh Prints]
    #26944593 - 09/19/20 05:13 PM (3 years, 4 months ago)

I have 2 pound bags.  What would be good for that?


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InvisiblePastywhyteMDiscord
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Re: Liquid culture [Re: Surfingmycelium]
    #26944748 - 09/19/20 06:59 PM (3 years, 4 months ago)

Quote:

Surfingmycelium said:
I have 2 pound bags.  What would be good for that?




A trash can.

But seriously, assuming it’s a 2 pound grain bag you likely want to spawn that to a coir verm sub at a 1:3 ratio. I would suggest a 40ish quart mono would be the ticket. Or make a larger mono and use both bags on it, however that carries more risk.


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Offlineseand04
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Re: Liquid culture [Re: Pastywhyte]
    #26944879 - 09/19/20 08:26 PM (3 years, 4 months ago)

Here is some LC I made with water, honey, and BRF. inoculated with clean agar plates.


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InvisibleThe Fresh PrintsS
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Re: Liquid culture [Re: seand04]
    #26944965 - 09/19/20 09:19 PM (3 years, 4 months ago)

Are you dropping whole plates into your LC??


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Offlinepolyflakes
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Re: Liquid culture [Re: The Fresh Prints]
    #26944978 - 09/19/20 09:26 PM (3 years, 4 months ago)

Quote:

Genghis Chron said:
Are you dropping whole plates into your LC??



Thats what I was looking at lol.


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Offlineseand04
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Re: Liquid culture [Re: polyflakes]
    #26944990 - 09/19/20 09:31 PM (3 years, 4 months ago)

Yea, I have a bunch :aweohyou:


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OfflineSurfingmycelium
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Re: Liquid culture [Re: seand04]
    #26946663 - 09/20/20 06:30 PM (3 years, 4 months ago)

How many CCs do you use?  I don’t know why mine isn’t colonizing.  I also put an entire agar plate in one and the same thing.  Nothing.  It’s been a week.  Maybe it just takes a while?  When I did my first grow with a MS syringe it was like gang busters right away.


Edited by Surfingmycelium (09/20/20 06:41 PM)


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Offlinemangaka911
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Re: Liquid culture [Re: Surfingmycelium]
    #26946730 - 09/20/20 07:07 PM (3 years, 4 months ago)

You used too many CC’s


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OfflineSurfingmycelium
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Re: Liquid culture [Re: mangaka911]
    #26946744 - 09/20/20 07:13 PM (3 years, 4 months ago)

I only used 2 this last time.  Is 2 ccs too much for a two pound grain bag?


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Offlinemangaka911
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Re: Liquid culture [Re: Surfingmycelium]
    #26946748 - 09/20/20 07:14 PM (3 years, 4 months ago)

2-3 CC’s will be enough to colonize as mycelium travels fast


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OfflineSurfingmycelium
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Re: Liquid culture [Re: mangaka911]
    #26946761 - 09/20/20 07:19 PM (3 years, 4 months ago)

Yeah.  But it hasn’t done anything in a week.  I would think it was quicker than spores.  I don’t know.  I guess I’ll keep waiting.


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InvisiblePastywhyteMDiscord
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Re: Liquid culture [Re: Surfingmycelium]
    #26946765 - 09/20/20 07:20 PM (3 years, 4 months ago)

If the LC is contamed or the colony in it lacks vigor it can be slower or an utter failure. If it’s axenic and I’m confident with it it will usually recover in 24-48 hours.


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OfflineSurfingmycelium
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Re: Liquid culture [Re: Pastywhyte]
    #26946814 - 09/20/20 07:56 PM (3 years, 4 months ago)

I put it on agar first to make sure it wasn’t contaminated since I used a ms syringe to inoculate it.  I now realize that was a newb mistake but it grew out without contams in the agar.  I shook up the bag today to see if there was any growth in the center and nothing but a few wet rye berries at the bottom.  I don’t know.  Maybe it’s a failure.  I’ll just let it sit and see what happens.  Tomorrow will be 7 days.


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InvisibleThe Fresh PrintsS
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Re: Liquid culture [Re: Surfingmycelium]
    #26946820 - 09/20/20 08:03 PM (3 years, 4 months ago)

If the LC didn't recover after 3 days I dont think you'll see anything. Good news is you have clean growth on agar. Make a transfer if necessary or just chop it up and drop some wedges to grain. Heck, drop a little bit in some fresh LC broth if you want to.


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Offlineseand04
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Re: Liquid culture [Re: The Fresh Prints]
    #26947094 - 09/21/20 12:19 AM (3 years, 4 months ago)

My jars are opaque after 2 days of shaking my LC is raw sugar,honey, and BRF, food coloring.


Edited by seand04 (09/21/20 12:23 AM)


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