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OfflineUcucu
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Registered: 02/14/20
Posts: 16
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Assorted Noobie Questions
    #26946588 - 09/20/20 05:33 PM (3 years, 4 months ago)

Hi,

I've been reading a bunch and tried cultivating several batches, more often than not I end up with contamination, which I think might be because of too much humidity. Anyways, there are some questions I have that I haven't been able to find a clear answer for, please help:

1) After soaking rye, is it necessary to boil it? Frank's 12 step guide says yes, the No Cook WBS guide says no, and I'm confused. I think I've been having humidity troubles in my last batches because of this.

2) When sterilizing rye (or wbs), is it necessary to use aluminum foil on top of jar lids with polyfilled 1/4" holes? I haven't done it most of the times because I'm lazy and haven't noticed a tangible difference.

3) Is there any particular reason why it's recommended that jars be filled to 75% capacity? Why not top it off?

4) Why is agar considered safer for inoculation than injecting spores through an injection port or through polyfill? I've done both, and injecting LOOKS safer, because the needle is sterilized with a flame and that's the only part that goes into the jar, whereas with agar i have to open the agar petri dish, cut out a piece, open the jar and place the piece in the jar, exposing it to the air in my still air box for much longer than injecting spores.

5) Any tips for jump starting stalled jars? They don't look contaminated, but seem to have stopped growing, despite looking humid and having a polyfill gas exchange hole at the top.

Thanks!


Edited by Ucucu (09/20/20 05:35 PM)


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OfflineForresterM
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Registered: 02/05/13
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Re: Assorted Noobie Questions [Re: Ucucu]
    #26946594 - 09/20/20 05:39 PM (3 years, 4 months ago)

1.  I had good results with doing a simmer.  But you can probably find a no-boil write up.  Methods vary.

2.  Probably not but it helps keep your polyfill dry(ish).  If you don't have any problems leaving it off, leave it off.

3.  So you can shake them after they cool, and while colonizing.  Full jars you can't shake.

4.  Because spores in solution are not sterile and usually contain bacteria and other contamination.

5.  They're probably contaminated.

Hope this helps :thumbup:


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Repugnant is a creature who would squander the ability to lift an eye to heaven, conscious of his fleeting time here.
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Have some medicinal mushrooms and want to get the most out of them?  Try this double extraction method.


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OfflineUcucu
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Registered: 02/14/20
Posts: 16
Last seen: 3 years, 1 month
Re: Assorted Noobie Questions [Re: Forrester]
    #26946789 - 09/20/20 07:34 PM (3 years, 4 months ago)

Thank you!


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