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OfflineDeadManSmurfing
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Registered: 07/25/20
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spores not germinating
    #26946379 - 09/20/20 03:17 PM (3 years, 4 months ago)

So I got spores through one of the Shroomery sponsors, and haven't been able to germinate them on agar? It's been about 20-21 days now and not even a single contaminant has appeared, let alone any whispy myc...

I'm thinking maybe the syringes ended up sterilizing in the mail or something? The outside temperature the day they were delivered was about 45C (113 F), and they were very warm when i removed them from the package, but I'm not sure what exact temperature they reached. I haven't been able to find any definite resources showing for sure what temperature range these spores can survive if exposed shortly. Could it be possible they were killed in the heat?

i am using a high nutrient agar recipe with 5 different syringes. 3 dishes for each syringe. Nothing has changed at all.


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InvisibleDoctor Mario
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Registered: 08/07/20
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Re: spores not germinating [Re: DeadManSmurfing]
    #26946432 - 09/20/20 03:45 PM (3 years, 4 months ago)

What is the recipe? You would be better off using a print vs a syringe.


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Offlinetripdawg420
low life with no life
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Registered: 02/02/09
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Re: spores not germinating [Re: Doctor Mario]
    #26946449 - 09/20/20 03:54 PM (3 years, 4 months ago)

when useing syringe to agar u use drips meaning after u flame the needle tip u want to squirt a small amount out the tip before u use it and u dont have to flame inbeteen jars since the needle never touches anything just make sure u squirt some out to get spores flowing thru the tip :thumbup:


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coir tek
http://www.shroomery.org/forums/showflat.php/Number/11917410
results :thumbup:


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Offlinedirtybirdx
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Registered: 07/28/20
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Re: spores not germinating [Re: tripdawg420]
    #26946460 - 09/20/20 04:04 PM (3 years, 4 months ago)

I hate syringes for pretty much everything, so I always try to get around them if I can.

If I want to take a spire syringe to a plate, hereโ€™s what I do:

- In SAB squirt a few ccโ€™s of the solution into an empty sterile Petri dish. 
- then I just take an inoculation loop, scoop up some spores, and streak them onto the agar.
- aside from the annoying syringes that are impossible to dispense a single drop, you also have the issue of not actually shooting any spores out with your solution.

Iโ€™ve found germinating spores to be easier if I go for the big clumps and try to see the spores with my naked eye.  Thatโ€™s easy to do with this method.


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