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InvisibleJosex
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Re: My journal [Re: nogoodnamesleft]
    #26945674 - 09/20/20 08:01 AM (3 years, 4 months ago)

Quote:

nogoodnamesleft said:
Well, I did some experiments on shaking the jars and it makes a huge difference to do the big jars twice.

So now that I saw what an improvement it made I shook the other jars to redistribute the mycelium again



My first round of quarts is almost 100% colonized.

The goal of all this is to try and get some done by Halloween.




No more A guys here.

Blessed be thee.
:pope:


Quote:

nogoodnamesleft said:
Some other jars I have going.




Looking forward to my filter patch bags showing up!



:freewilly:


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Offlinenogoodnamesleft
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Re: My journal [Re: Josex]
    #26945709 - 09/20/20 08:22 AM (3 years, 4 months ago)

A guy died


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InvisibleThomsen
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Re: My journal [Re: nogoodnamesleft]
    #26945837 - 09/20/20 09:38 AM (3 years, 4 months ago)

Quote:

nogoodnamesleft said:
A guy died




As he should. Take 5 :mushroom2: for coming to the brigt side!


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Re: My journal [Re: Thomsen]
    #26945841 - 09/20/20 09:42 AM (3 years, 4 months ago)

Question for the more practiced

Will the .5 micron filter that I ordered on these bags be too large for keeping contams etc out? 

Is it important to get the .2 micron bags? 

I think I can call unicorn bag and change my order because they are on COVID shipping times.


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InvisiblePastywhyteMDiscord
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Re: My journal [Re: nogoodnamesleft]
    #26945854 - 09/20/20 09:48 AM (3 years, 4 months ago)

I’ve used the 0.5 micron before and they were okay. No real issues compared to the 0.2. I’d use em.


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Re: My journal [Re: Pastywhyte]
    #26945874 - 09/20/20 09:57 AM (3 years, 4 months ago)

Quote:

Pastywhyte said:
I’ve used the 0.5 micron before and they were okay. No real issues compared to the 0.2. I’d use em.





Thank you for the prompt response :smile:

My plan for cultivation going forward :

Begin with agar plates of either isolated spore swab or cloned fruit

Prepare bags of grain spawn

Spawn bags with the plates of agar

Fruit in a grow room


——


I am considering building out a conex box for cultivation.

I can make a room for sterile work, one for fruiting, and one for storing colonizing grain pretty easily inside one and can land them to my ranch for around 3000 dollars. 

Any input from the community on this idea ?

I end up taking projects way too far sometimes but that’s also part of the fun of it for me

setting up all the trick infrastructure to do the project really gets me juiced up


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InvisiblePastywhyteMDiscord
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Re: My journal [Re: nogoodnamesleft]
    #26945891 - 09/20/20 10:10 AM (3 years, 4 months ago)

Personally I find agar wedges (even a whole plate) to be rather slow for bags. I want bags colonized ASAP. Liquids are my preferred way to expand a culture to bags.


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Offlinenogoodnamesleft
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Re: My journal [Re: Pastywhyte]
    #26945895 - 09/20/20 10:13 AM (3 years, 4 months ago)

Quote:

Pastywhyte said:
Personally I find agar wedges (even a whole plate) to be rather slow for bags. I want bags colonized ASAP. Liquids are my preferred way to expand a culture to bags.




Should I make a slurry from the plates ?


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InvisiblePastywhyteMDiscord
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Re: My journal [Re: nogoodnamesleft] * 1
    #26945925 - 09/20/20 10:27 AM (3 years, 4 months ago)

You could. It would provide a lot more inoculation points than just wedges will.


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Re: My journal [Re: Pastywhyte]
    #26946247 - 09/20/20 01:50 PM (3 years, 4 months ago)

Could I use peroxyacetic acid to clean my blender in front of the flow hood and use distilled water in the blender and then suck the slurry up right out of the blender carafe ?

http://www.eberbachlabtools.com/Lab-Blenders/Waring-1-Liter-Blenders/E8120-Waring-Blender.html

http://www.eberbachlabtools.com/Lab-Blenders/Blender-Containers/E8495-Waring-250ml-Blending-Container.html

This is the combo of parts I’m putting together. 

May seem like over kill but there’s adaptability to my horticulture day job so the investment in to the lab blender and portable hood are so I can work on tissue explants as well.


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OfflineForresterM
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Re: My journal [Re: nogoodnamesleft]
    #26946564 - 09/20/20 05:11 PM (3 years, 4 months ago)

I would just make a liquid culture if you've got a flowhood and good sterile technique, but that's just me.  I'm an LC lover and it seems simpler than cleaning blenders.  Blenders are nasty unless it's a magic bullet, you can't get in there...


--------------------
Repugnant is a creature who would squander the ability to lift an eye to heaven, conscious of his fleeting time here.
-------------------

Have some medicinal mushrooms and want to get the most out of them?  Try this double extraction method.


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Offlinenogoodnamesleft
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Re: My journal [Re: Forrester]
    #26946747 - 09/20/20 07:14 PM (3 years, 4 months ago)

Get in to where? 

The equipment I linked is able to be completely broken down and cleaned. 

The base adapts right to a mason jar too.

Seems pretty clutch to me to be able to put the jar already on the blender base with water in the PC 

Then make slurry with agar in front of the hood, take a Syringe and load it from the jar that’s only been opened once in front of the hood since PC and suck up the slurry and go to town on the bags?

The lab blender base is for my day job anyway. The cool jar adapter ( second link ) would apply to this hobby


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OfflineForresterM
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Re: My journal [Re: nogoodnamesleft]
    #26948014 - 09/21/20 03:34 PM (3 years, 4 months ago)

Yep, do whatever works best for ya.  I just like LC.


--------------------
Repugnant is a creature who would squander the ability to lift an eye to heaven, conscious of his fleeting time here.
-------------------

Have some medicinal mushrooms and want to get the most out of them?  Try this double extraction method.


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Re: My journal [Re: Forrester]
    #26949070 - 09/22/20 10:14 AM (3 years, 4 months ago)

Quote:

Forrester said:
Yep, do whatever works best for ya.  I just like LC.




Can you outline the pros vs con of agar slurry versus liquid culture


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Re: My journal [Re: nogoodnamesleft]
    #26949193 - 09/22/20 11:41 AM (3 years, 4 months ago)

Can someone tell me if these are exudates or contams






On another note, the tray I spawned outside is looking healthy after a few days



Edited by nogoodnamesleft (09/22/20 11:47 AM)


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InvisibleJosex
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Re: My journal [Re: nogoodnamesleft]
    #26949197 - 09/22/20 11:43 AM (3 years, 4 months ago)

Those jars are definitely toast. Looks like some type of mold and also bacteria, better luck next time.


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Re: My journal [Re: Josex]
    #26949523 - 09/22/20 02:52 PM (3 years, 4 months ago)

Ok got it. 

I’ll still spawn this stuff to some coco and just put it outside. 

I have another set of jars that’s doing well so I will just have to work from those :smile:


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OfflineForresterM
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Re: My journal [Re: nogoodnamesleft]
    #26949530 - 09/22/20 02:53 PM (3 years, 4 months ago)

Quote:

nogoodnamesleft said:
Can you outline the pros vs con of agar slurry versus liquid culture




There was a whole discussion on this fairly recently, I don't have a link but it's not far back if you search a bit


--------------------
Repugnant is a creature who would squander the ability to lift an eye to heaven, conscious of his fleeting time here.
-------------------

Have some medicinal mushrooms and want to get the most out of them?  Try this double extraction method.


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Offlinenogoodnamesleft
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Re: My journal [Re: Forrester]
    #26952420 - 09/24/20 09:35 AM (3 years, 4 months ago)

On a plus side the other jars are almost ready to be spawned.

Trying to decide if I should do trust dusty nursery flats or do a deeper Rubbermaid dish tub with all the jars together.

Surface area vs substrate depth and spawn density...always an interesting experiment



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Re: My journal [Re: nogoodnamesleft]
    #26953237 - 09/24/20 06:32 PM (3 years, 4 months ago)

The jar I threw down seems to be growing healthy



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