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Daddy4487
The creator

Registered: 08/18/20
Posts: 406
Last seen: 7 days, 15 hours
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Trichoderma or whatever the heck this is
#26945296 - 09/20/20 01:03 AM (3 years, 4 months ago) |
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Okay soo I’m trying to do my first successful grow from agar to harvest... the problem I seem to be having no matter what is contamination! Trichoderma or whatever this green stuff is..... here’s what I do
1-start with Pennington’s wbs. Measure it out, soak for 8 hours minimum. Remove floaters and rinse throughly 3+ times
2-Slowly summer with minimal heat until I notice the first sign of BURSTED(not exploded) kernels
3- immediately remove from heat, pour out all water, then transfer to a colander where I shake thoroughly straining all water and allowing steam to assist drying... spread out on a towel until just dry to touch ( i check moisture with tissues)
4- when I’m happy with the exterior dryness, I’ll jar up the seed, and pressure cook with lids upside down holes in them with either polyfil or micro pore tape, and wrapped in foil for 90 minutes at 15 psi
5- after done I remover and allow pressure to drop and remove jars , shaking a couple times while grains cool.
6- I’ve done both agar inoculation and G2G both unsuccessful.. I use a small room with a homemade SAB. I throughly clean everything with alcohol and heat any razors and or forceps with an alcohol lit flame for appx 5 minutes.
7- I try being as quick as possible with inoculation.
Yet this is my result every time... any suggestions??
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-------------------- Hope I can help & Thanks for the help!
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Thomsen



Registered: 09/27/19
Posts: 364
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Re: Trichoderma or whatever the heck this is [Re: Daddy4487]
#26945327 - 09/20/20 01:21 AM (3 years, 4 months ago) |
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Usually when you keep getting contaminated you look at too things as the first; your sterilization of the grains and the agar work.
How many jars do you do at the time? I'd do 2,5 hours of sterilizing at 15 psi when doing 10 jars. I'd do it even if i did less jars.
For how long do you PC your agar? Usually pre-mixed mixes says 15 min at 15 psi, but i give mine 30 mins. Works great.
Do you have pics of your agar? Have you made a no template control to see if it's there which it goes wrong?
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Daddy4487
The creator

Registered: 08/18/20
Posts: 406
Last seen: 7 days, 15 hours
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Re: Trichoderma or whatever the heck this is [Re: Thomsen]
#26945459 - 09/20/20 02:38 AM (3 years, 4 months ago) |
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The agar is good... it’s from a reliable source who doesn’t have any issue with it. I usually do 5-7 jars for 90 minutes. I figured it was either my grain or my SAB work... after reading a bunch of posts I think I’m going to rebuild my SAB as I misunderstood what it’s purpose was essentially
-------------------- Hope I can help & Thanks for the help!
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sporecap
Shedding...

Registered: 07/30/18
Posts: 413
Loc: Europe
Last seen: 1 year, 10 months
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Re: Trichoderma or whatever the heck this is [Re: Daddy4487]
#26945464 - 09/20/20 02:51 AM (3 years, 4 months ago) |
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You can keep a control jar after the PCing, so one jar that you treat identical to all others but this one you don't inocculate. Just leave it and check whether it contams or not. If it does, you know that your grain sterilization did not work, or your lid filters are bunk. If it stays fine, it is either your agar/culture or something gets in during inocculation in the SAB.
Edited by sporecap (09/20/20 02:51 AM)
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Forrester
aspiring sociopath


Registered: 02/05/13
Posts: 9,351
Loc: Northeast USA
Last seen: 1 month, 12 days
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Re: Trichoderma or whatever the heck this is [Re: Daddy4487]
#26945516 - 09/20/20 04:53 AM (3 years, 4 months ago) |
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Quote:
Daddy4487 said: after reading a bunch of posts I think I’m going to rebuild my SAB as I misunderstood what it’s purpose was essentially
This is very common, once you get that right it should help a lot.
Here's how I did mine back in the day. It's only one way, there's many:
-------------------- Repugnant is a creature who would squander the ability to lift an eye to heaven, conscious of his fleeting time here. ------------------- Have some medicinal mushrooms and want to get the most out of them? Try this double extraction method.
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Thomsen



Registered: 09/27/19
Posts: 364
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Re: Trichoderma or whatever the heck this is [Re: Forrester]
#26945525 - 09/20/20 05:11 AM (3 years, 4 months ago) |
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Quote:
Forrester said:
Quote:
Daddy4487 said: after reading a bunch of posts I think I’m going to rebuild my SAB as I misunderstood what it’s purpose was essentially
This is very common, once you get that right it should help a lot.
Here's how I did mine back in the day. It's only one way, there's many:

How would you inoculate a bag with agar in this one? Tilt the bag on the side and 'throw' the puck in seems like an easy way to push contamination inside it.
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A.k.a
Stranger



Registered: 10/27/19
Posts: 16,816
Loc: Gaming the system
Last seen: 1 day, 2 hours
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Re: Trichoderma or whatever the heck this is [Re: Thomsen]
#26945535 - 09/20/20 05:44 AM (3 years, 4 months ago) |
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Make sure you’re venting the pc and not starting the 90min timer until it’s at 15psi.
Mold is super frustrating.
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LAGM2020     
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Forrester
aspiring sociopath


Registered: 02/05/13
Posts: 9,351
Loc: Northeast USA
Last seen: 1 month, 12 days
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Re: Trichoderma or whatever the heck this is [Re: Thomsen]
#26945540 - 09/20/20 05:55 AM (3 years, 4 months ago) |
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Quote:
Thomsen said: How would you inoculate a bag with agar in this one? Tilt the bag on the side and 'throw' the puck in seems like an easy way to push contamination inside it.
Personally, I wouldn't. SAB's are notoriously inconvenient for working with bags at all. Although if that was your goal, you could design one for it I'm sure. Mine was for working with agar and innoculating jars of grain spawn.
-------------------- Repugnant is a creature who would squander the ability to lift an eye to heaven, conscious of his fleeting time here. ------------------- Have some medicinal mushrooms and want to get the most out of them? Try this double extraction method.
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