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OfflineRondo49
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Ms to agar possible contamination
    #26945417 - 09/20/20 02:15 AM (3 years, 4 months ago)

These were made on Thursday. Any idea was this is ?

Noced up on Thursday 9/17/2020


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OfflinePearl
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Re: Ms to agar possible contamination [Re: Rondo49]
    #26945573 - 09/20/20 06:34 AM (3 years, 4 months ago)

Bacteria

Was this from a syringe?


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OfflineMycobro420
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Re: Ms to agar possible contamination [Re: Pearl]
    #26945667 - 09/20/20 07:59 AM (3 years, 4 months ago)

Iv Ben growing for a while now and I would never take ms syring to agar.ever time I hear about it. It always looks like this. I can do agar pretty well but I doubt I could clean a plate with that much bacteria. I don't even see any myc.


Edited by Mycobro420 (09/20/20 08:00 AM)


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OfflineRondo49
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Re: Ms to agar possible contamination [Re: Pearl]
    #26945728 - 09/20/20 08:35 AM (3 years, 4 months ago)

Yeah from a mss. Kinda over did it with the squirt. In your opinion what's the best way to get an agar going?


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OfflineRondo49
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Re: Ms to agar possible contamination [Re: Mycobro420]
    #26945732 - 09/20/20 08:39 AM (3 years, 4 months ago)

What's your preferred method ?


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OfflineMycobro420
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Re: Ms to agar possible contamination [Re: Rondo49]
    #26945768 - 09/20/20 09:03 AM (3 years, 4 months ago)

I like cloning fresh Ms fruits.but I have also use colonized grain.never trued sterilized swabs but I heard those work good too


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OfflinePearl
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Re: Ms to agar possible contamination [Re: Mycobro420]
    #26945932 - 09/20/20 10:31 AM (3 years, 4 months ago)

I've read it recommended (Bod?) that you can sterilize a shot glass/similar in a PC cycle, move to SAB/flow hood, squirt the syringe solution into the sterilized vessel, and use an innoc loop from solution to agar. Then, try and isolate from there.

I've not tried it yet, but could be worth a shot. Could also pf tek it and clone a fruit...?


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InvisibleMycolorado
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Re: Ms to agar possible contamination [Re: Pearl]
    #26945941 - 09/20/20 10:35 AM (3 years, 4 months ago)

Or just put a drop on the plate and use a loop to streak in a zigzag from one end to the other.


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OfflineFredsGotSlacks
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Re: Ms to agar possible contamination [Re: Rondo49]
    #26946798 - 09/20/20 07:40 PM (3 years, 4 months ago)

OP, I can relate to the struggle with what you’re posting about. I’m just beginning this awesome adventure and ordered MS Syringes from NOT a trusted vendor  :facepalm3: and put a drop to agar.  Ended up with almost exactly what you have, except a lot more yellowy.  Researched and found out, yup it’s bacterial. 
Thought OK, maybe it’s my technique, tried again except this time with a blank dish to see if I was screwing up the agar.  Nope, same thing. Tried with Pasty Plates instead of pouring.  Same.  Tried one last time doing swabs to plates, same bacterial shit.
Finally came to the conclusion, my supplier was crap and sent me 5 bacterial ms syringes.
Now I’m just waiting for one of the Sponsors to lift their shipping restrictions to shipping “internationally” before ordering prints instead of syringes.
So I can feel your pain, but my advice, make multiple attempts to make sure it’s not your issue with being sterile or something else in your control before maybe switching suppliers or switch to prints.  Just wanted to share the steuggle is real my friend.  Good luck


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OfflineAlsetAlokin
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Re: Ms to agar possible contamination [Re: FredsGotSlacks]
    #26946813 - 09/20/20 07:52 PM (3 years, 4 months ago)

BRF pucks

https://www.shroomery.org/forums/showflat.php/Number/24806569

Had a run of extremely bacterial plates before I found that you can use BRF as a replacement to agar and it's extremely inhabitable to bacteria and very habitable for mycellium.

If it weren't for BRF pucks I probably would have given up trying to clean up the dirty syringes.

Edit: Also anecdotal, but agar made using BRF as a nutrition source seems to have some antibacterial properties IME. Run alongside MEA I've seen hitch-hiking bacteria get outrun by myc on BRF agar.


Edited by AlsetAlokin (09/20/20 08:01 PM)


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OfflineRondo49
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Re: Ms to agar possible contamination [Re: FredsGotSlacks]
    #26947728 - 09/21/20 12:35 PM (3 years, 4 months ago)

Thanks! I appreciate it. I think I'm just gonna stick to mss straight to jar and transfer some myc grains to agar.


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