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John in WI
Neo-Luddite


Registered: 12/18/13
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Acidified media--how low can the pH go for cubes?
#26944434 - 09/19/20 03:48 PM (3 years, 4 months ago) |
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I haven't found an answer to this after searching and googling the subject. I'm curious if anyone has done any work with acidifying agar or LC solutions for growing cubensis mycellium.
From what I understand, it's an old mycologist trick to inhibit bacteria. Certainly their is a point at which you are also inhibiting or even killing the mycellium. Just curious how low you could go. I found chemical suppliers selling nutrient agar mixes that go as low as pH 4. Would cubes survive at that pH? I'm guessing at low pH, it would inhibit germinating spores, but for a2a transfers, would that work?
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tryptkaloids
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Registered: 02/08/15
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Re: Acidified media--how low can the pH go for cubes? [Re: John in WI]
#26944465 - 09/19/20 03:59 PM (3 years, 4 months ago) |
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Idk... Sounds about right
-------------------- "Remember, kids, the difference between science and screwing around is writing it down" -adam savage Flowchart for Recommended plan of action. Learn the tried and true way to grow mushrooms Use the Damn search engine After you know what you're doing, take a break Pick a book, Make some chips! Josex said:Don't take the site seriously bro, ain't worth it.
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John in WI
Neo-Luddite


Registered: 12/18/13
Posts: 357
Loc: On a hippy trail head ful...
Last seen: 2 months, 3 days
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Re: Acidified media--how low can the pH go for cubes? [Re: tryptkaloids]
#26945907 - 09/20/20 10:21 AM (3 years, 4 months ago) |
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I had several LCs that looked cloudy, so presumably were contaminated with bacteria (haven't tested them--but they don't look good. The LC started off crystal clear).
I did a little math, and sterilized a syringe of 5% vinegar, and added enough to the jars that it should result in a solution ~pH=4. (I didn't want to put a grimey pH probe in the solution, and didn't know the precise volume of the LC, so it's a guestimate, but should be close).
We'll see what happens. I'm hoping they clear up, the myc takes over, and things are cool. Not super optimistic, but it's worth trying and knocking up some pints of grain. The literature suggests the bacteria should die, and biofilms should dissolve on the time scale of 10 minutes. Another paper suggested that in LCs, psylicybin concentration in mycellium was maximized at a pH~4.2. It wasn't the fastest growth rate (which seemed to be around pH 6), but it was measurable.
I'll keep everyone updated. I'm not going all in--this is an experiment--but if I can inhibit the bacteria to the extent that mycellium can outrun it, it would be an interesting trick in the toolbox.
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