Home | Community | Message Board

Magic Mushrooms Zamnesia
This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Left Coast Kratom Buy Kratom Extract, Kratom Powder For Sale   Mushroom-Hut Liquid Cultures, Mono Tub Substrate   North Spore Injection Grain Bag, North Spore Mushroom Grow Kits & Cultivation Supplies

Jump to first unread post Pages: 1
OfflineJohn in WI
Neo-Luddite
 User Gallery

Registered: 12/18/13
Posts: 357
Loc: On a hippy trail head ful...
Last seen: 2 months, 3 days
Acidified media--how low can the pH go for cubes?
    #26944434 - 09/19/20 03:48 PM (3 years, 4 months ago)

I haven't found an answer to this after searching and googling the subject.  I'm curious if anyone has done any work with acidifying agar or LC solutions for growing cubensis mycellium.

From what I understand, it's an old mycologist trick to inhibit bacteria.  Certainly their is a point at which you are also inhibiting or even killing the mycellium.  Just curious how low you could go.  I found chemical suppliers selling nutrient agar mixes that go as low as pH 4.  Would cubes survive at that pH?  I'm guessing at low pH, it would inhibit germinating spores, but for a2a transfers, would that work?


Extras: Filter Print Post Top
Offlinetryptkaloids
Learner
I'm a teapot


Registered: 02/08/15
Posts: 12,641
Loc: Exact Center
Last seen: 2 days, 10 hours
Trusted Cultivator
Re: Acidified media--how low can the pH go for cubes? [Re: John in WI]
    #26944465 - 09/19/20 03:59 PM (3 years, 4 months ago)

Idk... Sounds about right


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 


Extras: Filter Print Post Top
OfflineJohn in WI
Neo-Luddite
 User Gallery

Registered: 12/18/13
Posts: 357
Loc: On a hippy trail head ful...
Last seen: 2 months, 3 days
Re: Acidified media--how low can the pH go for cubes? [Re: tryptkaloids]
    #26945907 - 09/20/20 10:21 AM (3 years, 4 months ago)

I had several LCs that looked cloudy, so presumably were contaminated with bacteria (haven't tested them--but they don't look good.  The LC started off crystal clear).

I did a little math, and sterilized a syringe of 5% vinegar, and added enough to the jars that it should result in a solution ~pH=4.  (I didn't want to put a grimey pH probe in the solution, and didn't know the precise volume of the LC, so it's a guestimate, but should be close).

We'll see what happens.  I'm hoping they clear up, the myc takes over, and things are cool.  Not super optimistic, but it's worth trying and knocking up some pints of grain.  The literature suggests the bacteria should die, and biofilms should dissolve on the time scale of 10 minutes.  Another paper suggested that in LCs, psylicybin concentration in mycellium was maximized at a pH~4.2.  It wasn't the fastest growth rate (which seemed to be around pH 6), but it was measurable. 

I'll keep everyone updated.  I'm not going all in--this is an experiment--but if I can inhibit the bacteria to the extent that mycellium can outrun it, it would be an interesting trick in the toolbox.


Extras: Filter Print Post Top
Jump to top Pages: 1

Shop: Left Coast Kratom Buy Kratom Extract, Kratom Powder For Sale   Mushroom-Hut Liquid Cultures, Mono Tub Substrate   North Spore Injection Grain Bag, North Spore Mushroom Grow Kits & Cultivation Supplies


Similar ThreadsPosterViewsRepliesLast post
* pH up BikeCourier 726 11 01/31/03 11:57 AM
by SubGen1us
* product to lower ph? *DELETED* supernaut 939 8 01/17/05 05:22 PM
by Arch_Stanton
* whats the best liquid media??? felixhigh 1,548 16 06/10/12 02:34 PM
by VaeVictum
* Lime/ PH reciepe Vertigo 1,049 7 06/08/03 08:12 PM
by KlingonFromUranus
* Incubation Temp. Is Too Low? MoneyAddyct 1,103 16 10/07/04 06:19 PM
by Sam1912
* H20 pH is really 5 or so?! cheesenoonions 1,644 9 10/17/02 08:36 PM
by drenaline
* Almost free PH testers MadHatR 686 4 12/01/04 08:52 PM
by MadHatR
* POLL::: << Do you test your casing ph??? >> phucknut68 1,251 15 08/02/04 09:34 PM
by phucknut68

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
89 topic views. 20 members, 170 guests and 48 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.023 seconds spending 0.01 seconds on 14 queries.