Home | Community | Message Board


This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Left Coast Kratom Buy Kratom Extract   Original Sensible Seeds Bulk Cannabis Seeds   Mushroom-Hut Liquid Cultures   North Spore Cultivation Supplies

Jump to first unread post Pages: 1 | 2 | Next >  [ show all ]
OfflineSurfingmycelium
Stranger
Registered: 07/03/20
Posts: 181
Last seen: 1 year, 1 month
Liquid culture
    #26944402 - 09/19/20 03:25 PM (3 years, 4 months ago)

Hey guys,

  I made liquid culture with a PE ms syringe  a few months ago and inoculated two 3 pound bags.  I read to use 7-10 CCs so I used around 7.  The one bag grew a thick white mold all around it.  It didn’t seem like mycelium.  It looked more like a marshmallow consistency.  I let it sit for about a month to see if any fruit grew just in case but nothing so I tossed it.  The other one was just wet looking all the time and nothing ever happened. 

My liquid culture has thick mycelium in it and I put a few drops on a Petri dish to let it grow out first to make sure it didn’t have any contamination since I am a newb and used a MS syringe to inoculate the Liquid nutrients to make the LC. 

I think the issue was too much moisture in the bags maybe?

So, about a week ago I used the same LC and only used 2 CCs this time and the bag isn’t showing any growth at all.  Just a few wet grains.  I also put a few drops on a Petri dish again and it is growing on that with no issues but not my Rye grain bag I got from a reputable place. 

Any ideas on what I’m doing wrong?


Extras: Filter Print Post Top
Offlinetripdawg420
low life with no life
Male


Registered: 02/02/09
Posts: 7,071
Loc: illinois
Last seen: 12 hours, 20 minutes
Re: Liquid culture [Re: Surfingmycelium] * 1
    #26944405 - 09/19/20 03:28 PM (3 years, 4 months ago)

i would forget about any thing u heard about lc learn agar :thumbup:


--------------------
HUSTLER
How U Survive This Life Everyday Resourcefully
epic GT mono tub
http://www.shroomery.org/forums/showflat.php/Number/17277772

wbs tek
http://www.shroomery.org/forums/showflat.php/Number/11525679
coir tek
http://www.shroomery.org/forums/showflat.php/Number/11917410
results :thumbup:


Extras: Filter Print Post Top
InvisibleThe Fresh PrintsS
Smell ya later
Male User Gallery


Registered: 05/19/12
Posts: 1,377
Loc: Bel-Air Flag
Trusted Cultivator
Re: Liquid culture [Re: tripdawg420] * 1
    #26944420 - 09/19/20 03:36 PM (3 years, 4 months ago)

Well not necessarily. If you're going to make LC, use a clean culture from agar. Not a spore syringe. Also, sterilize your own grains. You're paying way too much to have someone do effortless work for you.


--------------------


Extras: Filter Print Post Top
Offlinetripdawg420
low life with no life
Male


Registered: 02/02/09
Posts: 7,071
Loc: illinois
Last seen: 12 hours, 20 minutes
Re: Liquid culture [Re: The Fresh Prints]
    #26944424 - 09/19/20 03:42 PM (3 years, 4 months ago)

lc has to many contam vectors if u was to leaen agar and use a wedge to innoc a gm and them g2g is a way better way of growing
but yes if ur going to use lc use a wedge then u wight as well g2g:thumbup:


--------------------
HUSTLER
How U Survive This Life Everyday Resourcefully
epic GT mono tub
http://www.shroomery.org/forums/showflat.php/Number/17277772

wbs tek
http://www.shroomery.org/forums/showflat.php/Number/11525679
coir tek
http://www.shroomery.org/forums/showflat.php/Number/11917410
results :thumbup:


Extras: Filter Print Post Top
OfflineSurfingmycelium
Stranger
Registered: 07/03/20
Posts: 181
Last seen: 1 year, 1 month
Re: Liquid culture [Re: The Fresh Prints]
    #26944427 - 09/19/20 03:44 PM (3 years, 4 months ago)

Oh I know.  And the place I’m getting it from always sends me 2 pound bags rather then 3 and I have to email them and they apologize and send me more 2 pound bags to compensate.  Lol.  But anyway, I can’t find a pressure canner right now.  They have disappeared off the face of the earth.  Lol. 

I actually made another LC with an agar piece from a GT clone.  It was the 3rd transfer, so hopefully that will work out. 

I just don’t understand why it doesn’t work.


Extras: Filter Print Post Top
Offlinetripdawg420
low life with no life
Male


Registered: 02/02/09
Posts: 7,071
Loc: illinois
Last seen: 12 hours, 20 minutes
Re: Liquid culture [Re: Surfingmycelium]
    #26944429 - 09/19/20 03:46 PM (3 years, 4 months ago)

u did all that w out a pc ur a monster :headbang3:


--------------------
HUSTLER
How U Survive This Life Everyday Resourcefully
epic GT mono tub
http://www.shroomery.org/forums/showflat.php/Number/17277772

wbs tek
http://www.shroomery.org/forums/showflat.php/Number/11525679
coir tek
http://www.shroomery.org/forums/showflat.php/Number/11917410
results :thumbup:


Extras: Filter Print Post Top
OfflineSurfingmycelium
Stranger
Registered: 07/03/20
Posts: 181
Last seen: 1 year, 1 month
Re: Liquid culture [Re: tripdawg420]
    #26944437 - 09/19/20 03:49 PM (3 years, 4 months ago)

No.  Lol.  I bought the liquid culture solution already made off the same site.  Very expensive for basically sugar water.  I got the agar dishes off amazon.  All I can do until a find a large pressure cooker


Extras: Filter Print Post Top
Offlinetripdawg420
low life with no life
Male


Registered: 02/02/09
Posts: 7,071
Loc: illinois
Last seen: 12 hours, 20 minutes
Re: Liquid culture [Re: Surfingmycelium]
    #26944440 - 09/19/20 03:50 PM (3 years, 4 months ago)

oh ok have u check amazon for a pc i just seen them at walmart the other day or truevalue or rual king


--------------------
HUSTLER
How U Survive This Life Everyday Resourcefully
epic GT mono tub
http://www.shroomery.org/forums/showflat.php/Number/17277772

wbs tek
http://www.shroomery.org/forums/showflat.php/Number/11525679
coir tek
http://www.shroomery.org/forums/showflat.php/Number/11917410
results :thumbup:


Extras: Filter Print Post Top
OfflineSurfingmycelium
Stranger
Registered: 07/03/20
Posts: 181
Last seen: 1 year, 1 month
Re: Liquid culture [Re: tripdawg420]
    #26944449 - 09/19/20 03:53 PM (3 years, 4 months ago)

Well I need a larger one.  Like a canner size.  16-23 qt range.  Those are the ones that are hard to get right now.


Extras: Filter Print Post Top
Offlinetripdawg420
low life with no life
Male


Registered: 02/02/09
Posts: 7,071
Loc: illinois
Last seen: 12 hours, 20 minutes
Re: Liquid culture [Re: Surfingmycelium]
    #26944454 - 09/19/20 03:54 PM (3 years, 4 months ago)

that sux man :sad:


--------------------
HUSTLER
How U Survive This Life Everyday Resourcefully
epic GT mono tub
http://www.shroomery.org/forums/showflat.php/Number/17277772

wbs tek
http://www.shroomery.org/forums/showflat.php/Number/11525679
coir tek
http://www.shroomery.org/forums/showflat.php/Number/11917410
results :thumbup:


Extras: Filter Print Post Top
OfflineSurfingmycelium
Stranger
Registered: 07/03/20
Posts: 181
Last seen: 1 year, 1 month
Re: Liquid culture [Re: tripdawg420]
    #26944459 - 09/19/20 03:57 PM (3 years, 4 months ago)

I know.  Just throwing money away until then. lol


Extras: Filter Print Post Top
Offlinetripdawg420
low life with no life
Male


Registered: 02/02/09
Posts: 7,071
Loc: illinois
Last seen: 12 hours, 20 minutes
Re: Liquid culture [Re: Surfingmycelium]
    #26944466 - 09/19/20 03:59 PM (3 years, 4 months ago)

:money:


--------------------
HUSTLER
How U Survive This Life Everyday Resourcefully
epic GT mono tub
http://www.shroomery.org/forums/showflat.php/Number/17277772

wbs tek
http://www.shroomery.org/forums/showflat.php/Number/11525679
coir tek
http://www.shroomery.org/forums/showflat.php/Number/11917410
results :thumbup:


Extras: Filter Print Post Top
OfflineSurfingmycelium
Stranger
Registered: 07/03/20
Posts: 181
Last seen: 1 year, 1 month
Re: Liquid culture [Re: tripdawg420]
    #26944470 - 09/19/20 04:01 PM (3 years, 4 months ago)

Yep.  Trying to learn so there’s a lot of trial and error, which costs money.  Lol.  But gotta do what you gotta do.  For now anyway.


Extras: Filter Print Post Top
OfflineSurfingmycelium
Stranger
Registered: 07/03/20
Posts: 181
Last seen: 1 year, 1 month
Re: Liquid culture [Re: Surfingmycelium]
    #26944474 - 09/19/20 04:03 PM (3 years, 4 months ago)

I actually put the rest of my agar dish into another bag and all the wedges stuck to the sides and it was super annoying.  I had to cut a slit in the bag and drop them in then fold the slit and gorilla tape around it 😂.  Let’s see if it contams out.  Did do it in a SAB so 🤞


Extras: Filter Print Post Top
Offlinetripdawg420
low life with no life
Male


Registered: 02/02/09
Posts: 7,071
Loc: illinois
Last seen: 12 hours, 20 minutes
Re: Liquid culture [Re: Surfingmycelium]
    #26944476 - 09/19/20 04:04 PM (3 years, 4 months ago)

:mushroom2:


--------------------
HUSTLER
How U Survive This Life Everyday Resourcefully
epic GT mono tub
http://www.shroomery.org/forums/showflat.php/Number/17277772

wbs tek
http://www.shroomery.org/forums/showflat.php/Number/11525679
coir tek
http://www.shroomery.org/forums/showflat.php/Number/11917410
results :thumbup:


Extras: Filter Print Post Top
OfflineSurfingmycelium
Stranger
Registered: 07/03/20
Posts: 181
Last seen: 1 year, 1 month
Re: Liquid culture [Re: tripdawg420]
    #26944544 - 09/19/20 04:42 PM (3 years, 4 months ago)

So liquid culture isn’t a good idea I’m guessing?


Extras: Filter Print Post Top
InvisiblemushboyMDiscord
modboy
 User Gallery


Registered: 04/24/05
Posts: 32,401
Loc: where?
Trusted Cultivator
OG Cultivator
Re: Liquid culture [Re: Surfingmycelium]
    #26944553 - 09/19/20 04:51 PM (3 years, 4 months ago)

Its a great idea if... IF you do it right.


Extras: Filter Print Post Top
InvisibleThe Fresh PrintsS
Smell ya later
Male User Gallery


Registered: 05/19/12
Posts: 1,377
Loc: Bel-Air Flag
Trusted Cultivator
Re: Liquid culture [Re: Surfingmycelium]
    #26944571 - 09/19/20 05:00 PM (3 years, 4 months ago)

Some swear by it, some don't see the point. There's more than one way to skin a cat. A2G > G2G works great, and its pretty fool proof. LC is fun because you can expand it like crazy fairly quickly, but you can't see contams until you put it to grain or test it on agar.

I personally don't bother with LC until I have a solid clone culture that I want to expand.


--------------------


Extras: Filter Print Post Top
OfflineSurfingmycelium
Stranger
Registered: 07/03/20
Posts: 181
Last seen: 1 year, 1 month
Re: Liquid culture [Re: The Fresh Prints]
    #26944581 - 09/19/20 05:07 PM (3 years, 4 months ago)

I put it on agar twice and no contams.  Just don’t know why it isn’t doing anything on the grain.  How many CCs do you guys use?


Extras: Filter Print Post Top
InvisibleThe Fresh PrintsS
Smell ya later
Male User Gallery


Registered: 05/19/12
Posts: 1,377
Loc: Bel-Air Flag
Trusted Cultivator
Re: Liquid culture [Re: Surfingmycelium]
    #26944585 - 09/19/20 05:10 PM (3 years, 4 months ago)

I use 3-5 cc's per quart of grain and then shake it up.

Pics?


--------------------


Edited by The Fresh Prints (09/19/20 05:11 PM)


Extras: Filter Print Post Top
Jump to top Pages: 1 | 2 | Next >  [ show all ]

Shop: Left Coast Kratom Buy Kratom Extract   Original Sensible Seeds Bulk Cannabis Seeds   Mushroom-Hut Liquid Cultures   North Spore Cultivation Supplies


Similar ThreadsPosterViewsRepliesLast post
* Spores, G2G, Liquid Culture, Cloning & Colonization Time.
( 1 2 3 all )
agar 52,876 43 07/08/23 07:04 AM
by Screwup
* Liquid Culture Challenges Leimana 9,591 4 05/31/05 11:33 PM
by Corporal Kielbasa
* 1st Liquid Culture, arrrgh! Seril 14,347 10 02/28/06 08:35 PM
by Limelight_Liver
* Liquid Culture Recipes MrMaddHatter 23,944 13 03/29/05 04:53 AM
by Sbealzebub
* Clear liquid cultures ? mickywilliams2 3,680 11 10/12/05 05:02 PM
by Bamaman
* i can get liquid cultures, but not grain laughingbuddha 2,812 8 03/08/05 12:02 PM
by laughingbuddha
* how to use cardboard cultures to inoculate grain jars and how to use a liquid culture to inoculate lordoftheshroomz 6,521 12 05/08/05 03:55 PM
by lordoftheshroomz
* Liquid culture parameters
( 1 2 all )
mogur 10,114 25 02/27/06 10:38 PM
by mogur

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
347 topic views. 35 members, 181 guests and 33 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.031 seconds spending 0.008 seconds on 16 queries.