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Offlinenogoodnamesleft
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My journal
    #26943601 - 09/19/20 06:14 AM (3 years, 4 months ago)

It's been about 12 years since since a guy has tried this kind of project.

a friend blessed a guy with some grain spawn that is colonized.  The original jars were multiplied in to what is documented below

varieties are Golden Teacher and Penis Envy.

Grain spawn is Rye berries.



Edited by nogoodnamesleft (09/19/20 02:40 PM)


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InvisibleJosex
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Re: My journal [Re: nogoodnamesleft]
    #26943606 - 09/19/20 06:20 AM (3 years, 4 months ago)

:pope:


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Offlinenogoodnamesleft
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Re: My journal [Re: Josex]
    #26943850 - 09/19/20 10:39 AM (3 years, 4 months ago)

A small update with some other jars A guy has colonizing,

A guy got frisky and did some half gallons. Why not right?

A guy sterilized coco in a drink cooler last night so a guy is going to use other colonized Jars to spawn that and try that out.

A guy also ordered 1000 Unicorn Bag XL gussets bags with .5 micron filter patch, since you can’t really find jars right now.







A guy also will be ordering one of these just to make spawning and g2g transfer as well as agar work a possibility.

https://www.terrauniversal.com/portable-horizontal-cleanbooth.html

Thanks for joining along.


Edited by nogoodnamesleft (09/19/20 10:40 AM)


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Invisibleeatyualive
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Re: My journal [Re: nogoodnamesleft]
    #26944391 - 09/19/20 03:20 PM (3 years, 4 months ago)

:popcorn:


--------------------
EAT GETS SHIT DONE


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Offlinetryptkaloids
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Re: My journal [Re: eatyualive]
    #26944473 - 09/19/20 04:02 PM (3 years, 4 months ago)

I "a guy" the same as "swim"

...asking for swim :duckhunt:


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 


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Offlinenogoodnamesleft
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Re: My journal [Re: tryptkaloids]
    #26944498 - 09/19/20 04:17 PM (3 years, 4 months ago)

Quote:

tryptkaloids said:
I "a guy" the same as "swim"

...asking for swim :duckhunt:




They're friends.

a guy spawned 1 quart that had a little black contam started in it to a 10x20 nursery flat with coco fiber and a little soy bean meal. Probably didn't need to add any protein to the coco but a guy figured since everything's an experiment any ways that he would have some fun and try out what would happen.

First jars are 10 days in to colonizing and will be spawned at 14 days even if there's a little bit of uncolonized grain left.

A guy remembers spawning at anything over 85% colonized having generally solid luck in nursery flats.

He figures that the uncolonized grain from the spawn jar becomes food for the mycelium later as the grains get mixed in to the bulk substrate.


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Offlinetryptkaloids
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Re: My journal [Re: nogoodnamesleft]
    #26944537 - 09/19/20 04:38 PM (3 years, 4 months ago)

All of that sounds like an invitation for contams


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 


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Re: My journal [Re: tryptkaloids]
    #26944555 - 09/19/20 04:51 PM (3 years, 4 months ago)

Yep, uncolonized grains are food for mold as it is any nutritious stuff you add to the coir besides vermiculite.
Don't make it hard on yourself, it's as simple as waiting until the jars are 100% colonized and not throwing any stuff in the coir.


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Offlinenogoodnamesleft
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Re: My journal [Re: Josex]
    #26944613 - 09/19/20 05:32 PM (3 years, 4 months ago)

Quote:

tryptkaloids said:
All of that sounds like an invitation for contams




Oops.

Quote:

Josex said:
Yep, uncolonized grains are food for mold as it is any nutritious stuff you add to the coir besides vermiculite.
Don't make it hard on yourself, it's as simple as waiting until the jars are 100% colonized and not throwing any stuff in the coir.




Does vermiculite have nutritive content?

Would adding the soy meal be any different than mixing coir with manure or straw?

Does coco coir need to be used by itself?

A guy would have waited till 100% , and plans to on the rest of the jars, just wanted to try to spawn one tray with a jar that was going down hill to a tray instead of just taking a 0.

Outdoor temperatures are between 80 and 40 so a guy is keeping the tray on the deck.

A guy has 0 attachment to the success of this or any tray or jar as these experiments are just for fun.

--

Another set of jars got g2g transferred by a guy and he shook them today.


Edited by nogoodnamesleft (09/19/20 05:33 PM)


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InvisibleJosex
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Re: My journal [Re: nogoodnamesleft]
    #26944629 - 09/19/20 05:44 PM (3 years, 4 months ago)

Vermiculite is used to improve the consistency of a substrate and it also serves as a water reservoir. Vermiculite is a mineral and mycelium can utilize it but it's not really considered nutritious.

Coir can be used by itself and lots of people mix it with vermiculite too. It's a matter of preference and/or availability.

Adding that soy meal to the substrate sounds like an excellent way to set up a mold party. Straw and poo are pasteurized to make them contam resistant, but you can't pasteurize soy because it's going to mold out.

FYI, grains by themselves have all the nutrition mushrooms need and then some more. You don't need to be adding anything else, doing otherwise is asking for trouble.

I hope them guys with 0 attachments do good. :thedude:


Edited by Josex (09/19/20 05:53 PM)


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Offlinenogoodnamesleft
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Re: My journal [Re: Josex]
    #26944646 - 09/19/20 05:57 PM (3 years, 4 months ago)

A guy will do the rest of them on straight coir. 

That is what a guy has in excess availability to him.

FWIW - in horticulture, a guys day job, 100% coco coir is the preferred substrate. The coco Substrate has chips added and some long fibers mixed in with the pith.

A guy weighed these, they’re 1400g average weight and make 20L when fully expanded.

A guy also has access to smaller bricks of 100% pith.


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Re: My journal [Re: nogoodnamesleft] * 1
    #26944734 - 09/19/20 06:53 PM (3 years, 4 months ago)

Gawd reading A guys posts is fucking painful.


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Offlinenogoodnamesleft
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Re: My journal [Re: Pastywhyte]
    #26944926 - 09/19/20 08:56 PM (3 years, 4 months ago)

A guy will so better to improve his literacy


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Offlinetryptkaloids
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Re: My journal [Re: nogoodnamesleft]
    #26945155 - 09/19/20 11:53 PM (3 years, 4 months ago)

I think he means you should just own up to it. You're anonymous here


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
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Josex said:Don't take the site seriously bro, ain't worth it.
 


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Re: My journal [Re: Pastywhyte]
    #26945257 - 09/20/20 12:47 AM (3 years, 4 months ago)

Quote:

Pastywhyte said:
Gawd reading A guys posts is fucking painful.



:whathesaid:

This guy is gonna spend his time reading other posts...

WFG :smile:


--------------------
The Official TNF Reccomended Teks & Methods
Bod's Simplified Cultivation Methods <-- Awesome!!!
D3monics Perfect Transfers and Agar Tek

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Re: My journal [Re: Pastywhyte]
    #26945357 - 09/20/20 01:40 AM (3 years, 4 months ago)

Quote:

Pastywhyte said:
Gawd reading A guys posts is fucking painful.




Agreed :lol:

It looks like it's could be a nice grow though OP. :cheers:


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InvisibleJosex
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Re: My journal [Re: Pastywhyte]
    #26945495 - 09/20/20 04:14 AM (3 years, 4 months ago)

Quote:

Pastywhyte said:
Gawd reading A guys posts is fucking painful.



It hurts my feelings even more than SWIM or AFOAF. The pain is almost physical. I hope it won't scar me much.
:gocrythen:


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OfflineForresterM
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Re: My journal [Re: Josex]
    #26945514 - 09/20/20 04:49 AM (3 years, 4 months ago)

:cop: We know it's you man.  It's ok.  We're all friends here.


--------------------
Repugnant is a creature who would squander the ability to lift an eye to heaven, conscious of his fleeting time here.
-------------------

Have some medicinal mushrooms and want to get the most out of them?  Try this double extraction method.


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Offlinenogoodnamesleft
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Re: My journal [Re: Forrester]
    #26945647 - 09/20/20 07:45 AM (3 years, 4 months ago)

Well, I did some experiments on shaking the jars and it makes a huge difference to do the big jars twice.

So now that I saw what an improvement it made I shook the other jars to redistribute the mycelium again



My first round of quarts is almost 100% colonized.

The goal of all this is to try and get some done by Halloween.


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Offlinenogoodnamesleft
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Re: My journal [Re: nogoodnamesleft]
    #26945653 - 09/20/20 07:50 AM (3 years, 4 months ago)

Some other jars I have going.




Looking forward to my filter patch bags showing up!


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Re: My journal [Re: nogoodnamesleft]
    #26945674 - 09/20/20 08:01 AM (3 years, 4 months ago)

Quote:

nogoodnamesleft said:
Well, I did some experiments on shaking the jars and it makes a huge difference to do the big jars twice.

So now that I saw what an improvement it made I shook the other jars to redistribute the mycelium again



My first round of quarts is almost 100% colonized.

The goal of all this is to try and get some done by Halloween.




No more A guys here.

Blessed be thee.
:pope:


Quote:

nogoodnamesleft said:
Some other jars I have going.




Looking forward to my filter patch bags showing up!



:freewilly:


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Offlinenogoodnamesleft
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Re: My journal [Re: Josex]
    #26945709 - 09/20/20 08:22 AM (3 years, 4 months ago)

A guy died


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InvisibleThomsen
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Re: My journal [Re: nogoodnamesleft]
    #26945837 - 09/20/20 09:38 AM (3 years, 4 months ago)

Quote:

nogoodnamesleft said:
A guy died




As he should. Take 5 :mushroom2: for coming to the brigt side!


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Offlinenogoodnamesleft
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Re: My journal [Re: Thomsen]
    #26945841 - 09/20/20 09:42 AM (3 years, 4 months ago)

Question for the more practiced

Will the .5 micron filter that I ordered on these bags be too large for keeping contams etc out? 

Is it important to get the .2 micron bags? 

I think I can call unicorn bag and change my order because they are on COVID shipping times.


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Re: My journal [Re: nogoodnamesleft]
    #26945854 - 09/20/20 09:48 AM (3 years, 4 months ago)

I’ve used the 0.5 micron before and they were okay. No real issues compared to the 0.2. I’d use em.


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Offlinenogoodnamesleft
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Re: My journal [Re: Pastywhyte]
    #26945874 - 09/20/20 09:57 AM (3 years, 4 months ago)

Quote:

Pastywhyte said:
I’ve used the 0.5 micron before and they were okay. No real issues compared to the 0.2. I’d use em.





Thank you for the prompt response :smile:

My plan for cultivation going forward :

Begin with agar plates of either isolated spore swab or cloned fruit

Prepare bags of grain spawn

Spawn bags with the plates of agar

Fruit in a grow room


——


I am considering building out a conex box for cultivation.

I can make a room for sterile work, one for fruiting, and one for storing colonizing grain pretty easily inside one and can land them to my ranch for around 3000 dollars. 

Any input from the community on this idea ?

I end up taking projects way too far sometimes but that’s also part of the fun of it for me

setting up all the trick infrastructure to do the project really gets me juiced up


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Re: My journal [Re: nogoodnamesleft]
    #26945891 - 09/20/20 10:10 AM (3 years, 4 months ago)

Personally I find agar wedges (even a whole plate) to be rather slow for bags. I want bags colonized ASAP. Liquids are my preferred way to expand a culture to bags.


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Offlinenogoodnamesleft
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Re: My journal [Re: Pastywhyte]
    #26945895 - 09/20/20 10:13 AM (3 years, 4 months ago)

Quote:

Pastywhyte said:
Personally I find agar wedges (even a whole plate) to be rather slow for bags. I want bags colonized ASAP. Liquids are my preferred way to expand a culture to bags.




Should I make a slurry from the plates ?


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Re: My journal [Re: nogoodnamesleft] * 1
    #26945925 - 09/20/20 10:27 AM (3 years, 4 months ago)

You could. It would provide a lot more inoculation points than just wedges will.


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Offlinenogoodnamesleft
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Re: My journal [Re: Pastywhyte]
    #26946247 - 09/20/20 01:50 PM (3 years, 4 months ago)

Could I use peroxyacetic acid to clean my blender in front of the flow hood and use distilled water in the blender and then suck the slurry up right out of the blender carafe ?

http://www.eberbachlabtools.com/Lab-Blenders/Waring-1-Liter-Blenders/E8120-Waring-Blender.html

http://www.eberbachlabtools.com/Lab-Blenders/Blender-Containers/E8495-Waring-250ml-Blending-Container.html

This is the combo of parts I’m putting together. 

May seem like over kill but there’s adaptability to my horticulture day job so the investment in to the lab blender and portable hood are so I can work on tissue explants as well.


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Re: My journal [Re: nogoodnamesleft]
    #26946564 - 09/20/20 05:11 PM (3 years, 4 months ago)

I would just make a liquid culture if you've got a flowhood and good sterile technique, but that's just me.  I'm an LC lover and it seems simpler than cleaning blenders.  Blenders are nasty unless it's a magic bullet, you can't get in there...


--------------------
Repugnant is a creature who would squander the ability to lift an eye to heaven, conscious of his fleeting time here.
-------------------

Have some medicinal mushrooms and want to get the most out of them?  Try this double extraction method.


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Offlinenogoodnamesleft
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Re: My journal [Re: Forrester]
    #26946747 - 09/20/20 07:14 PM (3 years, 4 months ago)

Get in to where? 

The equipment I linked is able to be completely broken down and cleaned. 

The base adapts right to a mason jar too.

Seems pretty clutch to me to be able to put the jar already on the blender base with water in the PC 

Then make slurry with agar in front of the hood, take a Syringe and load it from the jar that’s only been opened once in front of the hood since PC and suck up the slurry and go to town on the bags?

The lab blender base is for my day job anyway. The cool jar adapter ( second link ) would apply to this hobby


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Re: My journal [Re: nogoodnamesleft]
    #26948014 - 09/21/20 03:34 PM (3 years, 4 months ago)

Yep, do whatever works best for ya.  I just like LC.


--------------------
Repugnant is a creature who would squander the ability to lift an eye to heaven, conscious of his fleeting time here.
-------------------

Have some medicinal mushrooms and want to get the most out of them?  Try this double extraction method.


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Offlinenogoodnamesleft
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Re: My journal [Re: Forrester]
    #26949070 - 09/22/20 10:14 AM (3 years, 4 months ago)

Quote:

Forrester said:
Yep, do whatever works best for ya.  I just like LC.




Can you outline the pros vs con of agar slurry versus liquid culture


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Re: My journal [Re: nogoodnamesleft]
    #26949193 - 09/22/20 11:41 AM (3 years, 4 months ago)

Can someone tell me if these are exudates or contams






On another note, the tray I spawned outside is looking healthy after a few days



Edited by nogoodnamesleft (09/22/20 11:47 AM)


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Re: My journal [Re: nogoodnamesleft]
    #26949197 - 09/22/20 11:43 AM (3 years, 4 months ago)

Those jars are definitely toast. Looks like some type of mold and also bacteria, better luck next time.


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Re: My journal [Re: Josex]
    #26949523 - 09/22/20 02:52 PM (3 years, 4 months ago)

Ok got it. 

I’ll still spawn this stuff to some coco and just put it outside. 

I have another set of jars that’s doing well so I will just have to work from those :smile:


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Re: My journal [Re: nogoodnamesleft]
    #26949530 - 09/22/20 02:53 PM (3 years, 4 months ago)

Quote:

nogoodnamesleft said:
Can you outline the pros vs con of agar slurry versus liquid culture




There was a whole discussion on this fairly recently, I don't have a link but it's not far back if you search a bit


--------------------
Repugnant is a creature who would squander the ability to lift an eye to heaven, conscious of his fleeting time here.
-------------------

Have some medicinal mushrooms and want to get the most out of them?  Try this double extraction method.


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Re: My journal [Re: Forrester]
    #26952420 - 09/24/20 09:35 AM (3 years, 4 months ago)

On a plus side the other jars are almost ready to be spawned.

Trying to decide if I should do trust dusty nursery flats or do a deeper Rubbermaid dish tub with all the jars together.

Surface area vs substrate depth and spawn density...always an interesting experiment



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Re: My journal [Re: nogoodnamesleft]
    #26953237 - 09/24/20 06:32 PM (3 years, 4 months ago)

The jar I threw down seems to be growing healthy



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Re: My journal [Re: nogoodnamesleft]
    #26953254 - 09/24/20 06:38 PM (3 years, 4 months ago)

It looks very dry that coir. You'd benefit from misting it down.


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Re: My journal [Re: Thomsen]
    #26953273 - 09/24/20 06:44 PM (3 years, 4 months ago)

Quote:

Thomsen said:
It looks very dry that coir. You'd benefit from misting it down.





Thanks for your input.

I think it’ll be fine. I work with this same coco on a daily basis for horticulture so I’m fairly decent at judging how hydrated it is.

There are some long fibers mixed in that are blond no matter how much you hydrate the coco. The pith itself is rich dark brown, because it is hydrated to field capacity.





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Re: My journal [Re: nogoodnamesleft]
    #26954019 - 09/25/20 08:01 AM (3 years, 4 months ago)

I don’t know jack shit about plants but I do feel that coir is looking dry af.


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Re: My journal [Re: Pastywhyte]
    #26954143 - 09/25/20 09:33 AM (3 years, 4 months ago)

Quote:

Pastywhyte said:
I don’t know jack shit about plants but I do feel that coir is looking dry af.







This picture better describes what I was explaining.

The pith itself is hydrated to field capacity. The fiber is blonde.


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Re: My journal [Re: nogoodnamesleft]
    #26954147 - 09/25/20 09:37 AM (3 years, 4 months ago)

It does look dry as hell to me too.


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Re: My journal [Re: Josex]
    #26954184 - 09/25/20 09:57 AM (3 years, 4 months ago)

If I wanted to add moisture should I add a broad spectrum bactericide like peroxyacetic acid to the water in the spray bottle.

I have 75 gallons of 15% concentrate on hand.

How much moisture should I add?

I have VWC meters I use to Monitor my substrate pots in my greenhouses , what % saturation VWC do you guys recommend for coco substrate for mushrooms?

There isn’t anything drawing down moisture from the substrate as I keep the tray covered with foil and when I hydrated the coco I used 7.5X the dry coir weight in water.

When I hydrate the discs for use in horticulture I use 5X and the VWC meter says 90% of water holding capacity had been reached


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Re: My journal [Re: nogoodnamesleft]
    #26954304 - 09/25/20 11:16 AM (3 years, 4 months ago)

Don't add anything to your tub other than water...

Spray it until  glistening.

The fanciest tools most of us use to read conditions is our eyes.

In mycology you want field capacity. Look it up.

There is a living organism in there... it consumes water just as we do


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Re: My journal [Re: nogoodnamesleft]
    #26954340 - 09/25/20 11:34 AM (3 years, 4 months ago)

Quote:

nogoodnamesleft said:
If I wanted to add moisture should I add a broad spectrum bactericide like peroxyacetic acid to the water in the spray bottle.

I have 75 gallons of 15% concentrate on hand.

How much moisture should I add?

I have VWC meters I use to Monitor my substrate pots in my greenhouses , what % saturation VWC do you guys recommend for coco substrate for mushrooms?

There isn’t anything drawing down moisture from the substrate as I keep the tray covered with foil and when I hydrated the coco I used 7.5X the dry coir weight in water.

When I hydrate the discs for use in horticulture I use 5X and the VWC meter says 90% of water holding capacity had been reached




I have no clue what you're talking about. It looks like you're making the mistake of mixing up horticulture and mushroom cultivation. The problem is that they're like apples and oranges. Very little (if anything) of what you know about plants will serve you for mushroom cultivation.

Start reading teks here.
Lots of people prep coir this way:
https://www.shroomery.org/forums/showflat.php/Number/11916595#11916595


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Re: My journal [Re: Josex]
    #26954452 - 09/25/20 12:50 PM (3 years, 4 months ago)

VWC stands for Volumetric Water Content...

Substrate water holding capacity can be measured by sensors called lysimeters. I use these in my day job. 

I read that bucket thread as well as another one that actually had some guideline water calculations that are right in line with what we do in horticulture in that they recommended 5X the dry bricks weight of water to use to hydrate the substrate.

The 1400g discs that I use fill a 20L container , in the greenhouse we use 2 gallons which is exactly 5X the dry weight. I did 10,500mL and there was water at the bottom of the cooler that the coco couldn’t absorb.


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Re: My journal [Re: tryptkaloids]
    #26954487 - 09/25/20 01:13 PM (3 years, 4 months ago)

Quote:

tryptkaloids said:
Don't add anything to your tub other than water...

Spray it until  glistening.

The fanciest tools most of us use to read conditions is our eyes.

In mycology you want field capacity. Look it up.

There is a living organism in there... it consumes water just as we do




Plants roots are a living organism, too. I understand that the mycelium consumes water. Mushrooms are 90% water by weight. I just mean relative to the substrate and it’s water content compared to the amount of mycelium growing on it I don’t think there’s been a significant dry down of the coco substrate because I have evaporation controlled.

I also know mycelium can move water through the substrate. Similar to how plants roots can.

Does mycelium colonize substrate faster in search of water?

The reason why I’m mentioning the VWC meters I use for horticulture is they quantify substrate moisture capacity. What you’re calling field capacity is when the moisture reading on the meter is above 80%

I understand watering the substrate after a flush just curious if there’s any benefit to watering during colonizing...does the substrate need to be held to field capacity the entire grow?

What approaches do commercial mushroom farms use to do this during their spawn run?

Thanks for the input.


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Re: My journal [Re: nogoodnamesleft]
    #26954526 - 09/25/20 01:46 PM (3 years, 4 months ago)

The closer it is to the right water content at spawning the easier it is to maintain surface conditions throughout  the first flush.

Misting isn't to water the mycelium, its to maintain proper Surface conditions


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Re: My journal [Re: tryptkaloids]
    #26954596 - 09/25/20 02:37 PM (3 years, 4 months ago)

Any time I lift the foil to check, it’s full of condensation.

The surface conditions thread you linked seems like it’s talking about during pinning.

I could understand wanting increased substrate moisture at that stage

Again I’ll ask the question -

Is there an ideal substrate volumetric water content for colonization prior to fruiting


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Re: My journal [Re: tryptkaloids]
    #26954647 - 09/25/20 03:07 PM (3 years, 4 months ago)

None of us can actually answer that question because  we use our senses, not technology which is more money than they are worth for this hobby.

Im sure there is, but most of us don't have any way of measuring such a thing

Would be interesting to know, but  I'm sure every culture is different.

But like I said before,
Quote:

tryptkaloids said:
The closer it is to the right water content at spawning the easier it is to maintain surface conditions throughout  the first flush.




Proper hydration  of your substrate has a major effect on speed, health, and conditions of your grow.


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Re: My journal [Re: tryptkaloids]
    #26954769 - 09/25/20 04:40 PM (3 years, 4 months ago)

Sounds like a nice opportunity for an experiment



In about a month my greenhouses will be in winter mode which means no growing all repairs and upgrades so my VWC meters will be free.

I could saturate coir to different VWC % and spawn with the same spawn and record the time to pinning for the different VWC %  and compare first flush yields.

The meters will also record by the minute how much moisture is being drawn down from the substrate.



If anyone else is interested in these meters they’re called TEROS-12 and they’re made by Meter Group ( very creative company name lol )


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Re: My journal [Re: nogoodnamesleft]
    #26954913 - 09/25/20 06:35 PM (3 years, 4 months ago)

Lmao, metergroup is local company for me.

Used to be called decagon devices which is arguably a much cooler name.

A friend of mine works there.

Ms cultures are too variant to say forsure what would be causing the better results


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Re: My journal [Re: tryptkaloids]
    #26955798 - 09/26/20 09:27 AM (3 years, 4 months ago)

If I used an isolate then would the experiment be valid?

I’m genuinely interested in this experiment as I have the equipment to do it.


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Re: My journal [Re: nogoodnamesleft]
    #26956069 - 09/26/20 12:45 PM (3 years, 4 months ago)

So I spawned the contaminated jars I asked about in a previous post to some coco just to see what would happen.  5.5 qts total spawn 2 just didn’t have enough healthy growth in them for me to use them.

The last jar I did To a nursery flat now just under a week later looks like by 2 weeks I will be able to put a nursery dome on it and mist it daily. It’s been growing on my deck with outside temps ranging from 45 at night to 85 during the day time.

I know coir has some anti bacterial and anti fungal properties. I’m curious if some contams can’t survive once spawned to coir allowing what healthy mycelium is alive to grow strong enough to out compete the booboo.

I used a cardboard diaper box and trash bag to do a big “beer box tek”...trash bag in the box coir and grain mixed in the trash bag then fold down the bag over the coco and wait


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Re: My journal [Re: nogoodnamesleft]
    #26956175 - 09/26/20 01:52 PM (3 years, 4 months ago)

Wow you're full of theories...

:Awemazing:


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Re: My journal [Re: Josex]
    #26956308 - 09/26/20 03:39 PM (3 years, 4 months ago)

@josex

I do have plenty. Here are some interesting reads on the inherent anti microbial / fungal / bacterial properties of coconut

https://www.sciencedirect.com/science/article/abs/pii/S0196978105002482

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3730797/

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4121915/

By trade I am an agronomist so when I approach this hobby I am doing it with the same lens, it makes it fun for me.


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Re: My journal [Re: nogoodnamesleft]
    #26956423 - 09/26/20 05:05 PM (3 years, 4 months ago)

Almost there with the good jars



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Re: My journal [Re: nogoodnamesleft]
    #26958138 - 09/27/20 11:10 PM (3 years, 3 months ago)

Spawned 3 quarts to 2 nursery flats of straight coco.

Everything I’ve spawned and tossed outside seems to be growing...

My goal of birthday boomers may happen. Sweet


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Re: My journal [Re: nogoodnamesleft]
    #26960742 - 09/29/20 05:25 PM (3 years, 3 months ago)





Looking pretty good here !

Hoping all my agar supplies and portable clean booth arrive soon.

I also ordered some rusty white and pf classic spores. That’s the first kind of cube I grew and it’s been about 17 years since I tried them. I ate a handful of aborts in a tea and had the strongest trip of my
Life that’s never been matched to date..and I’ve definitely tried. So I’m looking forward to taking those syringes to agar and playing around with that !


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Re: My journal [Re: nogoodnamesleft]
    #26964300 - 10/01/20 04:56 PM (3 years, 3 months ago)

I’ll let these incubate for another 5 days then put the dome on them and let them fruit





All kinds of goodies on the way with regards to cultivation supplies.

Pyrex media bottles
MEA blend 
Activated charcoal
20lbs of polyfil
Petri dishes
XLS-A and 3T bags (1000 of each)
Another 23 qt cooker

Decided on getting a Terra Universal laminar flood hood.

When I get plant tissue culture ramped up heavily at my day job I will order one of their clean rooms

Definitely jumping back in to doing this in some degree consistently


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Re: My journal [Re: nogoodnamesleft]
    #26964335 - 10/01/20 05:16 PM (3 years, 3 months ago)

Seedling flats eh? Ill never use poly again. Id also suggest looking up pastys ez agar, paatys ez dial tubs, holy grail plates. Ive also been using micropore again. Started in 2009 but got sick of it. Then started again 6-8 months ago. Sick of it again. Also lookup fruit at spawn by azure. Use a top layer of your sub as a pseudo casing. I go straight to fruiting conditions at spawn. And pastys ez dial tubs. Wont need anything else!


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Edited by eatyualive (10/04/20 11:58 AM)


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Re: My journal [Re: eatyualive]
    #26964608 - 10/01/20 07:20 PM (3 years, 3 months ago)

Thanks for all that solid advice eats.

My frame of reference for this is 2005 lol.


You’re the man.


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Re: My journal [Re: nogoodnamesleft]
    #26968850 - 10/04/20 10:15 AM (3 years, 3 months ago)

Here’s an experiment I know I’ll get ripped for but I’ll share anyway. 

The spawn jars previously shown that had gone bacterial I decided to spawn anyway because the ratio of healthy mycelium to funk was in favor of the fungi. 

At my day job I work with commercial bactericide/fungicide called peroxyacetic acid , it’s basically hydrogen peroxide and vinegar mixed together. I decided to see if this stuff has any effect on living mycelium at working concentrations. 

The 15% concentrate I use at work will burn a hole in your leg, I’m using 1% and diluting that 2mL in 1000mL so 1:500 ratio.

Anyway, I also have an abundance of coco so I just use that. The brand is HortGrow. It has an extremely high substrate water holding capacity. 1500 grams of dry compressed coco takes 9500mL water to reach field capacity. I verify this with a TEROS 12 substrate moisture sensor. 

This is pretty much 1 1500 Dry gram disc and I think about 6 quarts of spawn.

It seems that consistently misting the box with bactericidal water is letting the healthy mycelium colonize. 

GreenScissor Tote liner is used for wrapping the box and making it in to a fruiting chamber. 







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Re: My journal [Re: nogoodnamesleft]
    #26968991 - 10/04/20 11:55 AM (3 years, 3 months ago)

Oh nogo i remember man it has been some time and glad to see you back at it!

Nogo is an old buddy. That was the straw log days :lol:

Oh that is an interesting trial nogo.


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Re: My journal [Re: eatyualive]
    #26973325 - 10/07/20 06:57 AM (3 years, 3 months ago)



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Re: My journal [Re: nogoodnamesleft]
    #26973686 - 10/07/20 11:53 AM (3 years, 3 months ago)

Getting pins on the tray I spawned with the jar that had a little funk in it back on 9/19



My cardboard box is killing it too...



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Re: My journal [Re: nogoodnamesleft]
    #26974072 - 10/07/20 03:46 PM (3 years, 3 months ago)

Looking pretty wet in there. Mist less


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Re: My journal [Re: tryptkaloids]
    #26974128 - 10/07/20 04:28 PM (3 years, 3 months ago)

thanks for the advice.

I had literally just misted them, then whipped my phone out and took the photos.

I think it's working OK.

My flow hood arrives today from terra universal looking forward to making cultures and starting with agar to Grain.

I opened the lids on the humidity dome and stuffed with polyfil


Edited by nogoodnamesleft (10/07/20 05:00 PM)


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Re: My journal [Re: nogoodnamesleft] * 1
    #26975969 - 10/08/20 07:07 PM (3 years, 3 months ago)

My side experiment seems to be working ok. More and more healthy mycelium less yellow every day



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Re: My journal [Re: nogoodnamesleft] * 2
    #26980780 - 10/12/20 12:31 AM (3 years, 3 months ago)

Picked my first flush in 15 years second flush pinning right behind it



Also got my flow hood together and made a few different recipes of agar to experiment with



The recipe for 1 L was 30 G malt 15 G agar and 3g peptone


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Re: My journal [Re: nogoodnamesleft] * 1
    #26988854 - 10/16/20 04:53 PM (3 years, 3 months ago)



All the agar plates are growing mycelium - time to get serious !


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Re: My journal [Re: nogoodnamesleft]
    #26988857 - 10/16/20 04:55 PM (3 years, 3 months ago)

Well shit, mushrooms! :cool:


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Re: My journal [Re: Josex] * 1
    #26988948 - 10/16/20 06:04 PM (3 years, 3 months ago)

My goal of birthday boomers is realized and on top of that, I’ve gone fully in to the deep end


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Re: My journal [Re: nogoodnamesleft]
    #27019670 - 11/03/20 03:03 PM (3 years, 2 months ago)

:popcorn:

did that weirdo yellow contam box fruit?


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Re: My journal [Re: deadmandave] * 1
    #27019687 - 11/03/20 03:10 PM (3 years, 2 months ago)

Yes it did.

Repeated spray with PAA water turned the yellow white I went out of town and it got forgot about I came back and there were fruits in the box.



Edited by nogoodnamesleft (11/03/20 04:40 PM)


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Re: My journal [Re: nogoodnamesleft]
    #27020342 - 11/03/20 09:26 PM (3 years, 2 months ago)

:popcorn:


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Re: My journal [Re: filthyknees]
    #27025843 - 11/06/20 10:44 PM (3 years, 2 months ago)

More and more equipment landing every week.

This is like building a race car...when all the parts are finally assembled it’s going to be fucking awesome


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Re: My journal [Re: nogoodnamesleft]
    #27041395 - 11/15/20 11:28 PM (3 years, 2 months ago)

:threadmonitor:


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Re: My journal [Re: Mr. Mushie]
    #27041945 - 11/16/20 09:39 AM (3 years, 2 months ago)



Got a little bit too big of a splooge on the plate but damn they jumped off fast


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Re: My journal [Re: nogoodnamesleft]
    #27041988 - 11/16/20 10:09 AM (3 years, 2 months ago)

I wouldn't transfer from that at all


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Re: My journal [Re: tryptkaloids]
    #27042001 - 11/16/20 10:16 AM (3 years, 2 months ago)

Quote:

tryptkaloids said:
I wouldn't transfer from that at all




I’m going to, and it’ll probably grow some mushrooms just fine.


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Re: My journal [Re: nogoodnamesleft]
    #27042026 - 11/16/20 10:33 AM (3 years, 2 months ago)

I dont see any cube myc at all, but I'd love to see you prove me wrong


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Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 


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Re: My journal [Re: tryptkaloids]
    #27043068 - 11/16/20 10:05 PM (3 years, 2 months ago)

Yeah that’s not going to grow mushrooms


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Re: My journal [Re: Pastywhyte]
    #27065284 - 11/30/20 07:26 PM (3 years, 1 month ago)



More fucking around while we wait to unleash the kraken

May or may not grow some mushrooms


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Re: My journal [Re: nogoodnamesleft]
    #27073221 - 12/05/20 09:43 AM (3 years, 1 month ago)



PF Classic on straight coir with 3 quarts of oats.


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Re: My journal [Re: nogoodnamesleft]
    #27074767 - 12/06/20 08:04 AM (3 years, 1 month ago)

Started making LC broth. Didn’t like the flocculant from using LME so I made up some with Karo. 

Inoculating this with melmak from agar today



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Re: My journal [Re: nogoodnamesleft] * 1
    #27076735 - 12/07/20 08:31 AM (3 years, 1 month ago)

I inoculated 800mL of Karo broth this morning and threw my LME jars on the stir plate. 

I put .1g activated carbon in 500mL to see if the mycelium would grab it and start building its network around the carbon bits...well, it did.



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Re: My journal [Re: nogoodnamesleft] * 1
    #27082384 - 12/10/20 02:54 PM (3 years, 1 month ago)

I got cloning fruits to work



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Re: My journal [Re: nogoodnamesleft]
    #27086130 - 12/12/20 05:23 PM (3 years, 1 month ago)



PFC fruiting - this is a huge deal for me because back in 2005 I grew some PFC and the experience from those mushrooms was formative for my adult life and I really attribute the success I have today to that one mega trip.

I’m hoping these have a similar effect and power to what I had back then


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OfflineMr. Mushie

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Re: My journal [Re: nogoodnamesleft]
    #27086319 - 12/12/20 07:21 PM (3 years, 1 month ago)

Congrats on your soon to be fruits.

What shifted for you after experiencing that trip?


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Re: My journal [Re: Mr. Mushie] * 1
    #27086348 - 12/12/20 07:35 PM (3 years, 1 month ago)

Quote:

Mr. Mushie said:
Congrats on your soon to be fruits.

What shifted for you after experiencing that trip?





I realized life was a big video game and you can level up, skip levels, and even apply cheat codes to progress an objective to the finish...like quests in Zelda. So I applied that paradigm hard going forward and never set any limits on the quest


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Re: My journal [Re: nogoodnamesleft] * 1
    #27088600 - 12/14/20 10:59 AM (3 years, 1 month ago)

We’re getting there. 



I have agar plates ready to clone the better examples


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Re: My journal [Re: nogoodnamesleft]
    #27088795 - 12/14/20 12:38 PM (3 years, 1 month ago)

:cheers:


--------------------
But if you're in a hurry, and really got to go
If you're in a hurry, might have to find out slow
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You get there quicker just a step at a time
It's one thing to bark, another to bite
The show ain't over till you pack up at night


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Re: My journal [Re: filthyknees] * 1
    #27089442 - 12/14/20 07:44 PM (3 years, 1 month ago)

Quote:

filthyknees said:
:cheers:




Salute to you for your assistance with the process - all your posts are full of valuable information that is rooted in experience



Been practicing more commercial techniques in preparation for the arrival of The Dragon... my large Chinese autoclave spawn producing mega machine

I’m liking Milo and think that I will end up running with it for my grain spawn. The prep is too easy. 

I have made 10 2 kg bags , some 14A some 3T , some inoculated with LC some with agar wedge.

These will get LC.



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Re: My journal [Re: nogoodnamesleft]
    #27111286 - 12/27/20 08:01 PM (3 years, 1 month ago)



Melmak tray about to get cased and then fruited


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Re: My journal [Re: nogoodnamesleft]
    #27113061 - 12/28/20 08:35 PM (3 years, 30 days ago)

Rusty white

I think I I used too much spawn in this case



I think this bin is going to blob on me. We’ll see in the next week


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Re: My journal [Re: nogoodnamesleft]
    #27119879 - 01/01/21 08:23 AM (3 years, 27 days ago)

Making progress with agar and spores



Rusty White bin isn’t blobbing but growing like spaghetti instead...a tangled mess. LoL


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Re: My journal [Re: nogoodnamesleft] * 1
    #27125844 - 01/04/21 11:01 AM (3 years, 24 days ago)



Unmod tub


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Re: My journal [Re: nogoodnamesleft]
    #27142450 - 01/11/21 05:31 PM (3 years, 17 days ago)



I went ahead and fruit some melmak , culture is performing well.

I fixed my agar issues. Was drastically low on nutrition. Growth is much much improved


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Re: My journal [Re: nogoodnamesleft]
    #27142459 - 01/11/21 05:35 PM (3 years, 17 days ago)

Dude, nobody gave a hoot about this thread when you started it with the "a guy's..." thing.

You're making progress by leaps and bounds. :congrats:


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Re: My journal [Re: Josex]
    #27142544 - 01/11/21 06:49 PM (3 years, 17 days ago)

Quote:

Josex said:
Dude, nobody gave a hoot about this thread when you started it with the "a guy's..." thing.

You're making progress by leaps and bounds. :congrats:




Thanks.

It’s a fun hobby.


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Re: My journal [Re: nogoodnamesleft]
    #27158598 - 01/20/21 12:53 AM (3 years, 8 days ago)



I need 2 more of these while I wait for the dragon which now is delayed till March apparently


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Re: My journal [Re: nogoodnamesleft]
    #27159097 - 01/20/21 09:40 AM (3 years, 8 days ago)

I just ordered one yesterday.

Did it take about 3 months for them to show?

Mine should be in second week of april if the "60 business days" is accurate.


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Re: My journal [Re: Mr. Mushie]
    #27159664 - 01/20/21 02:04 PM (3 years, 8 days ago)

I got these off Craigslist if you can believe that


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Re: My journal [Re: nogoodnamesleft]
    #27160331 - 01/20/21 08:45 PM (3 years, 7 days ago)

Those things are in mint condition. Hell of a find sir.

I cannot find anything like that on craigslist in these parts.

You keep mentioning "the dragon" and I remember seeing you take delivery of some 55 gallon steamer (bubba?) either earlier in the thread or in another.

Is the dragon some sort of giant autoclave? If so, what made you go that route instead of just grabbing a few of the bubba's barrels (if that's even what you have)


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Re: My journal [Re: Mr. Mushie]
    #27161111 - 01/21/21 10:24 AM (3 years, 7 days ago)

The dragon is a 2500L capacity autoclave I am trying to import from China. Ocean freight is a bitch right now and I’m only occupying part of a container with my load. I either need to buy other stuff or another autoclave to simplify shipping logistics


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Re: My journal [Re: nogoodnamesleft]
    #27258687 - 03/18/21 10:41 AM (2 years, 10 months ago)

Your lab is bringing out some serious gear envy. Very nice!


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Re: My journal [Re: Hindsight]
    #27264189 - 03/22/21 08:07 AM (2 years, 10 months ago)



Release the kraken is the operative phrase here


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Re: My journal [Re: nogoodnamesleft]
    #27265718 - 03/23/21 08:54 AM (2 years, 10 months ago)





Malt Yeast Peptone been working excellent for me

I can’t get cultures to grow healthy on just LME and agar.

Hats off to those who can


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Re: My journal [Re: nogoodnamesleft]
    #27265924 - 03/23/21 11:41 AM (2 years, 10 months ago)

Was it you who posted the two 100L autoclaves in the daily cultivation or grow equipment thread maybe? I recognize the studs in your grow room :smile:


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Re: My journal [Re: FrugalFungi]
    #27267091 - 03/24/21 07:58 AM (2 years, 10 months ago)

Yes. 

One of the two came without baskets. Figuring out an alternative to use it ASAP


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Re: My journal [Re: FrugalFungi]
    #27280123 - 04/24/21 06:42 AM (2 years, 9 months ago)



Krakens in the water


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Re: My journal [Re: nogoodnamesleft]
    #27280837 - 04/24/21 09:04 PM (2 years, 9 months ago)

Looks lovely sir.

Spawning that many is a serious task if done alone.

All unmodded tubs?

You're doing LC to bags for all of these? Are you expanding the bags to G2 or G3?

I'm currently at a similar tub count but will be quadrupling in the coming months.

Curious about all the ways things can be done most efficiently at that scale.


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Re: My journal [Re: nogoodnamesleft]
    #27280900 - 04/24/21 10:31 PM (2 years, 8 months ago)

Quote:

nogoodnamesleft said:


Krakens in the water




Bro what in the fuck

6 months ago you were stumbling into it now this? You must be... very well connected lol. Respect


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[||||||||||||||||||||||||||||||||||||]
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Re: My journal [Re: Mr. Mushie]
    #27281264 - 04/25/21 08:40 AM (2 years, 8 months ago)

Quote:

Mr. Mushie said:
Looks lovely sir.

Spawning that many is a serious task if done alone.

All unmodded tubs?

You're doing LC to bags for all of these? Are you expanding the bags to G2 or G3?

I'm currently at a similar tub count but will be quadrupling in the coming months.

Curious about all the ways things can be done most efficiently at that scale.




Filthyknees coir trough tek is the only way to get this done in a timely fashion. I can do 20 tubs worth of spawn at once and just bucket it out. 

For now the tubs aren’t drilled

No LC to bag. 

Agar to grain master, then master jar to 12 bags.


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Re: My journal [Re: Benson]
    #27281272 - 04/25/21 08:48 AM (2 years, 8 months ago)

Quote:

Benson said:
Quote:

nogoodnamesleft said:


Krakens in the water




Bro what in the fuck

6 months ago you were stumbling into it now this? You must be... very well connected lol. Respect





The fuck is, I am determined and methodical so things like this spiral really fast in to going from training wheels to Aprilia RSV4


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Re: My journal [Re: nogoodnamesleft]
    #27281301 - 04/25/21 09:18 AM (2 years, 8 months ago)

Hope that didn't come across negative - I'm thoroughly impressed and inspired and would do the same if I had the space for it :grin:


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[||||||||||||||||||||||||||||||||||||]
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Re: My journal [Re: nogoodnamesleft]
    #27281498 - 04/25/21 12:23 PM (2 years, 8 months ago)

Quote:

nogoodnamesleft said:
Quote:

Mr. Mushie said:
Looks lovely sir.

Spawning that many is a serious task if done alone.

All unmodded tubs?

You're doing LC to bags for all of these? Are you expanding the bags to G2 or G3?

I'm currently at a similar tub count but will be quadrupling in the coming months.

Curious about all the ways things can be done most efficiently at that scale.




Filthyknees coir trough tek is the only way to get this done in a timely fashion. I can do 20 tubs worth of spawn at once and just bucket it out. 

For now the tubs aren’t drilled

No LC to bag. 

Agar to grain master, then master jar to 12 bags.




I'm doing the trough tek as well.

It still takes me about ~5 minutes to really get an even mix of the spawn and substrate, scoop out the spawn/sub that has fallen between the liner and wall of the tub, level, and apply the psuedo casing.

I'm very anal about the sub being level so I could probably shave some time if I was more lax about that.

I'm doing the same, A2G master -> 10-15 bags.


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Re: My journal [Re: Mr. Mushie]
    #27290152 - 05/02/21 09:01 AM (2 years, 8 months ago)

Yeah, I don't spend that much time on leveling it, seems to work out OK.

A Bunch more tubs about to get spawned.

I need to pull all the equipment out of my lab and put the linoleum down.


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Re: My journal [Re: nogoodnamesleft] * 1
    #27305600 - 05/12/21 10:59 AM (2 years, 8 months ago)



4 lb grain 11 lb coco verm in a 66 qt tub


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Re: My journal [Re: nogoodnamesleft]
    #27305984 - 05/12/21 04:12 PM (2 years, 8 months ago)



My experimental round is looking good

Grateful for the culture


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Re: My journal [Re: nogoodnamesleft]
    #27306106 - 05/12/21 05:53 PM (2 years, 8 months ago)

Nice man. :thumbup:

Melmak?

That yield makes me want to ditch all other kinds of cubes and just run that. The swabs someone sent me reverted so I gotta give it another go.

What's your tub capacity in that room? 175-200?

I ran some unmodded besides some EZ dial tubs with similar results.

Now i'm on the fence about modding all of my tubs going forward...


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Re: My journal [Re: Mr. Mushie]
    #27306429 - 05/12/21 11:08 PM (2 years, 8 months ago)

im trying a flipped lid. I drilled holes in some, some are just unlatched


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Re: My journal [Re: nogoodnamesleft]
    #27306446 - 05/12/21 11:42 PM (2 years, 8 months ago)

Coming to watch this unfold.
:thisisgonnabegood:


--------------------

Crack's Tek's and Shit



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Re: My journal [Re: Crackatoa] * 1
    #27316823 - 05/20/21 06:43 PM (2 years, 8 months ago)



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Re: My journal [Re: nogoodnamesleft]
    #27316848 - 05/20/21 07:15 PM (2 years, 8 months ago)

Cool thread NGNL! Thanks for sharing your journey with us! The Krakens is OUT! 

:vinsue:


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Re: My journal [Re: Atomsplit]
    #27317224 - 05/21/21 07:49 AM (2 years, 8 months ago)

I’m having a lot of fun with this -

Major tub situation going down right now.


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Re: My journal [Re: nogoodnamesleft]
    #27319278 - 05/22/21 08:57 PM (2 years, 8 months ago)



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Re: My journal [Re: nogoodnamesleft]
    #27319299 - 05/22/21 09:21 PM (2 years, 8 months ago)

Melmak iso?


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Re: My journal [Re: Mr. Mushie]
    #27319505 - 05/23/21 02:00 AM (2 years, 8 months ago)

:threadmonitor:


--------------------
(You Know What Time It Is)
Major Issues in the Psychedelic Movement: https://www.shroomery.org/forums/showflat.php/Number/27677086:elmo:
"You never have to prove the fool a fool, just let them speak."
Please, be an adult. Get vaccinated. Dont use psychedelics as an excuse. Dont come at me with some hippy dippy nonsense, GO GET VACCINATED.
Be Gay, Do Crime 161 1312


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Re: My journal [Re: nogoodnamesleft]
    #27319669 - 05/23/21 07:42 AM (2 years, 8 months ago)

Yes it’s either melmak 118 or melmak thrasher.

Idk which tubs are which because I didn’t mark the spawn but they seem pretty comparable


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Re: My journal [Re: nogoodnamesleft]
    #27321209 - 05/24/21 01:32 PM (2 years, 8 months ago)



Second flush


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Re: My journal [Re: nogoodnamesleft]
    #27325535 - 05/27/21 05:26 PM (2 years, 7 months ago)

Personal best



Looking forward to bettering it


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Re: My journal [Re: nogoodnamesleft]
    #27327628 - 05/29/21 07:28 AM (2 years, 7 months ago)



Tubbin’


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Re: My journal [Re: nogoodnamesleft]
    #27327637 - 05/29/21 07:37 AM (2 years, 7 months ago)

Quote:

nogoodnamesleft said:


Tubbin’




Killing it brother :rockon:


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Re: My journal [Re: Benson]
    #27327829 - 05/29/21 11:09 AM (2 years, 7 months ago)

:thatsjustswell:


--------------------

Crack's Tek's and Shit



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Re: My journal [Re: Crackatoa]
    #27333983 - 06/03/21 03:17 PM (2 years, 7 months ago)



Melmak dries up dense


Edited by nogoodnamesleft (06/03/21 03:17 PM)


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Re: My journal [Re: nogoodnamesleft]
    #27338967 - 06/07/21 01:49 PM (2 years, 7 months ago)

Penis Envy “original”



I prefer melmak - more yield and not so difficult to fruit.


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Re: My journal [Re: nogoodnamesleft]
    #27342243 - 06/09/21 09:49 PM (2 years, 7 months ago)






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Re: My journal [Re: nogoodnamesleft]
    #27342260 - 06/09/21 10:10 PM (2 years, 7 months ago)

:myman:


--------------------

Crack's Tek's and Shit



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Re: My journal [Re: nogoodnamesleft]
    #27342411 - 06/10/21 01:01 AM (2 years, 7 months ago)

Quote:

nogoodnamesleft said:


Melmak dries up dense




How's the potency on these suckers?


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Re: My journal [Re: Mr. Mushie]
    #27342710 - 06/10/21 08:53 AM (2 years, 7 months ago)

They’re active. LOL


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Re: My journal [Re: nogoodnamesleft]
    #27342741 - 06/10/21 09:12 AM (2 years, 7 months ago)

Nicely done :rockon:


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Re: My journal [Re: Pastywhyte]
    #27342838 - 06/10/21 10:03 AM (2 years, 7 months ago)

From Pasty saying this in the beginning of the thread:
Quote:

Pastywhyte said:
Gawd reading A guys posts is fucking painful.




To end up actually congratulating OP:

Quote:

Pastywhyte said:
Nicely done :rockon:





We've witnessed a quantum leap in this thread, nonames knows his crap now.
:cigkiss:


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Re: My journal [Re: Josex]
    #27344051 - 06/11/21 07:10 AM (2 years, 7 months ago)

Quote:

Josex said:
From Pasty saying this in the beginning of the thread:
Quote:

Pastywhyte said:
Gawd reading A guys posts is fucking painful.




To end up actually congratulating OP:

Quote:

Pastywhyte said:
Nicely done :rockon:





We've witnessed a quantum leap in this thread, nonames knows his crap now.
:cigkiss:





What’s good! Thanks for the positive encouragement. This is pretty fun. More improvements coming soon


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Re: My journal [Re: nogoodnamesleft]
    #27345270 - 06/12/21 08:49 AM (2 years, 7 months ago)



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Re: My journal [Re: Josex]
    #27345292 - 06/12/21 09:12 AM (2 years, 7 months ago)

Quote:

Josex said:
From Pasty saying this in the beginning of the thread:
Quote:

Pastywhyte said:
Gawd reading A guys posts is fucking painful.




To end up actually congratulating OP:

Quote:

Pastywhyte said:
Nicely done :rockon:





We've witnessed a quantum leap in this thread, nonames knows his crap now.
:cigkiss:




Indeed, I consider this to represent a resounding success.


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Re: My journal [Re: Pastywhyte]
    #27345837 - 06/12/21 05:32 PM (2 years, 7 months ago)

Thanks. Josex and Pastywhite


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Re: My journal [Re: nogoodnamesleft] * 1
    #28224024 - 03/10/23 03:55 PM (10 months, 14 days ago)




Life has its ups and downs and come back arounds so here we are again..

Got a few hundred pounds of milo sterilized and a bunch of quarts of grain ready to spread around on Monday.

Varieties this time are hillbilly pumpkin and penis envy.

Thanks to all.


Edited by nogoodnamesleft (03/10/23 03:57 PM)


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Re: My journal [Re: nogoodnamesleft]
    #28228311 - 03/13/23 06:45 PM (10 months, 11 days ago)



1:1 ratio of LME to dextrose , spun on stir plate once a day for about 15-30 seconds

Will be using a pipette to inoculate.


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Re: My journal [Re: nogoodnamesleft] * 1
    #28229363 - 03/14/23 01:02 PM (10 months, 10 days ago)

Completed inoculating all the sterilized grain I prepared. Did 1 quart to 6-7 bags which is twice as heavy as I normally expand my grain masters as I’m looking for a little more speed here.

I have 30 bags that I’m going to fruit for fun just to see what they do. 



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Re: My journal [Re: nogoodnamesleft]
    #28230694 - 03/15/23 01:16 PM (10 months, 9 days ago)

48 hours later….



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Re: My journal [Re: nogoodnamesleft]
    #28232215 - 03/16/23 03:13 PM (10 months, 8 days ago)



Liquid culture crushing it.

My next set of bags will be inoculated with this stuff


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Re: My journal [Re: nogoodnamesleft]
    #28232224 - 03/16/23 03:20 PM (10 months, 8 days ago)

Nice!  Have you tried sslc?


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Re: My journal [Re: Mycolorado]
    #28232466 - 03/16/23 06:38 PM (10 months, 8 days ago)

Yes but it’s less than ideal for my dispersion method so regular LC is fine



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Re: My journal [Re: nogoodnamesleft]
    #28232578 - 03/16/23 06:56 PM (10 months, 8 days ago)

Ah, gotcha.  Been looking for a good micropipette that’s not ridiculously pricey. How’s the microlit holding up?


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Re: My journal [Re: Mycolorado]
    #28233900 - 03/17/23 03:22 PM (10 months, 7 days ago)

Quote:

Mycolorado said:
Ah, gotcha.  Been looking for a good micropipette that’s not ridiculously pricey. How’s the microlit holding up?




Haven’t used it yet but it comes highly recommended


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Re: My journal [Re: nogoodnamesleft]
    #28234069 - 03/17/23 05:50 PM (10 months, 7 days ago)

Right on, I’m looking at a ward.  Let me know how you like it.


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Re: My journal [Re: Mycolorado]
    #28237014 - 03/19/23 05:48 PM (10 months, 5 days ago)







The black plate is a second transfer on LME with gentamicin, the clear plate is a transfer back to regular LME. 

I add AC to the plates I pour with gentamicin just so I know it’s treated. I’ve been considering dying my PDA bright blue but I probably won’t because it’ll be another step and thing to have to keep stocked in the lab. It’s white anyway so it’s obvious what recipe it is.

Bag is 4lbs of milo that I used about a pound of WBS to inoculate. I’ll shake it tomorrow for the last time.

I soaked about 200lbs of grain today, big bag cook tomorrow

I’ll be using LC for this batch


Edited by nogoodnamesleft (03/19/23 05:50 PM)


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Re: My journal [Re: nogoodnamesleft] * 2
    #28238193 - 03/20/23 03:12 PM (10 months, 4 days ago)

Almost 100% colonized on the last set of bags so it’s time to prep more so we have a nice workflow

Here’s a little pictorial. 

I use 5 gallon paint strainer bags with 20lbs in each bag.

The grain is submerged and agitated twice in clean water before the soak.



I use the hottest setting on my on demand heater and fill a cooler with water and submerge the bags for 18+ hours



I pull the bags and let them drain on plastic pallets for a while. Like 15-30 min



Disperse the grain on sheets from the Salvation Army ($4 king size sheets, can’t beat it)



Sort of obvious last step, but fill bags

I forgot to add, the grain usually hits a 45–50% increase in weight from water absorption this way, so one can plan how much to prep off that metric


Edited by nogoodnamesleft (03/20/23 06:04 PM)


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Re: My journal [Re: nogoodnamesleft]
    #28241429 - 03/22/23 01:41 PM (10 months, 2 days ago)



Trusty ol’ PE…love this one.


Edited by nogoodnamesleft (03/22/23 01:41 PM)


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Re: My journal [Re: nogoodnamesleft] * 2
    #28247559 - 03/26/23 12:39 PM (9 months, 29 days ago)

Getting after it now..

Time to cut liners and clean totes



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Re: My journal [Re: nogoodnamesleft]
    #28259824 - 04/02/23 09:30 PM (9 months, 21 days ago)

Salute to the legend for trough tek



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Re: My journal [Re: nogoodnamesleft]
    #28305755 - 05/03/23 11:40 PM (8 months, 21 days ago)





Time to start again.

More Hillbilly Pumpkin en masse

In testing I have APE221 , PE from a plate pin , PESA Boner rhizo, a plate pin from the main HBP culture


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Re: My journal [Re: nogoodnamesleft]
    #28364584 - 06/18/23 05:33 PM (7 months, 7 days ago)



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Re: My journal [Re: nogoodnamesleft] * 1
    #28364595 - 06/18/23 05:45 PM (7 months, 7 days ago)

Playing with LC



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Re: My journal [Re: nogoodnamesleft]
    #28364758 - 06/18/23 08:48 PM (7 months, 7 days ago)

Those look good


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Re: My journal [Re: Pastywhyte]
    #28371392 - 06/23/23 02:15 PM (7 months, 2 days ago)

Pins


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Re: My journal [Re: nogoodnamesleft]
    #28372469 - 06/24/23 09:38 AM (7 months, 1 day ago)

Hillbilly pumpkin. Might be phasing out this culture but then it’ll do this



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Re: My journal [Re: nogoodnamesleft]
    #28375250 - 06/26/23 12:56 PM (6 months, 30 days ago)

Flushes and stuff



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Re: My journal [Re: Pastywhyte]
    #28375456 - 06/26/23 03:27 PM (6 months, 30 days ago)

Quote:

Pastywhyte said:
Those look good




They all contam because of the lids


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Re: My journal [Re: nogoodnamesleft]
    #28376785 - 06/27/23 04:59 PM (6 months, 29 days ago)

More flushes…



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Re: My journal [Re: nogoodnamesleft]
    #28381666 - 07/01/23 05:46 PM (6 months, 25 days ago)










APE221 performing excellent

Bluey Vuitton needs more testing but probably not a long term candidate


Edited by nogoodnamesleft (07/01/23 05:47 PM)


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Re: My journal [Re: nogoodnamesleft]
    #28395516 - 07/14/23 03:24 PM (6 months, 12 days ago)

Progress and stuff



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Re: My journal [Re: nogoodnamesleft]
    #28401088 - 07/19/23 06:39 PM (6 months, 7 days ago)

Clusters and toadstools





Swabs made from the 100g individual as well as clone plates on PDYA

Cluster was dissected and the center mushroom and largest mushroom were cloned. The largest fruit in the cluster was 74g also done on PDYA

Clones from the 402g fruit are growing out clean and have been transferred. LC will be made in another transfer or two


Edited by nogoodnamesleft (07/19/23 07:44 PM)


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Re: My journal [Re: nogoodnamesleft] * 2
    #28402885 - 07/21/23 01:09 PM (6 months, 5 days ago)

Some specimens







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Re: My journal [Re: nogoodnamesleft] * 2
    #28409617 - 07/26/23 09:05 PM (6 months, 5 minutes ago)

Really liking the APE culture.

Took a clone from a 250g clustered fruit. Once that tests out, if the results are favorable, I’ll make some swabs and go to spore and select clones again for size and cluster formation







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Re: My journal [Re: nogoodnamesleft]
    #28505274 - 10/15/23 09:18 AM (3 months, 12 days ago)







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Re: My journal [Re: nogoodnamesleft]
    #28508192 - 10/17/23 01:50 PM (3 months, 10 days ago)

Dayumn. Some monsters. What strain is that?


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Re: My journal [Re: deadmandave] * 1
    #28529241 - 11/04/23 03:37 PM (2 months, 23 days ago)





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Re: My journal [Re: nogoodnamesleft]
    #28538694 - 11/11/23 12:50 PM (2 months, 16 days ago)

Hooooly shit lol :eek:


--------------------


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Re: My journal [Re: MeiSucks]
    #28608429 - 01/03/24 12:41 PM (25 days, 7 hours ago)



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