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InvisibleMycolorado
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Re: My journal [Re: nogoodnamesleft]
    #28234069 - 03/17/23 05:50 PM (10 months, 7 days ago)

Right on, I’m looking at a ward.  Let me know how you like it.


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Offlinenogoodnamesleft
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Re: My journal [Re: Mycolorado]
    #28237014 - 03/19/23 05:48 PM (10 months, 5 days ago)







The black plate is a second transfer on LME with gentamicin, the clear plate is a transfer back to regular LME. 

I add AC to the plates I pour with gentamicin just so I know it’s treated. I’ve been considering dying my PDA bright blue but I probably won’t because it’ll be another step and thing to have to keep stocked in the lab. It’s white anyway so it’s obvious what recipe it is.

Bag is 4lbs of milo that I used about a pound of WBS to inoculate. I’ll shake it tomorrow for the last time.

I soaked about 200lbs of grain today, big bag cook tomorrow

I’ll be using LC for this batch


Edited by nogoodnamesleft (03/19/23 05:50 PM)


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Offlinenogoodnamesleft
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Re: My journal [Re: nogoodnamesleft] * 2
    #28238193 - 03/20/23 03:12 PM (10 months, 4 days ago)

Almost 100% colonized on the last set of bags so it’s time to prep more so we have a nice workflow

Here’s a little pictorial. 

I use 5 gallon paint strainer bags with 20lbs in each bag.

The grain is submerged and agitated twice in clean water before the soak.



I use the hottest setting on my on demand heater and fill a cooler with water and submerge the bags for 18+ hours



I pull the bags and let them drain on plastic pallets for a while. Like 15-30 min



Disperse the grain on sheets from the Salvation Army ($4 king size sheets, can’t beat it)



Sort of obvious last step, but fill bags

I forgot to add, the grain usually hits a 45–50% increase in weight from water absorption this way, so one can plan how much to prep off that metric


Edited by nogoodnamesleft (03/20/23 06:04 PM)


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Offlinenogoodnamesleft
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Re: My journal [Re: nogoodnamesleft]
    #28241429 - 03/22/23 01:41 PM (10 months, 2 days ago)



Trusty ol’ PE…love this one.


Edited by nogoodnamesleft (03/22/23 01:41 PM)


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Re: My journal [Re: nogoodnamesleft] * 2
    #28247559 - 03/26/23 12:39 PM (9 months, 29 days ago)

Getting after it now..

Time to cut liners and clean totes



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Re: My journal [Re: nogoodnamesleft]
    #28259824 - 04/02/23 09:30 PM (9 months, 21 days ago)

Salute to the legend for trough tek



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Re: My journal [Re: nogoodnamesleft]
    #28305755 - 05/03/23 11:40 PM (8 months, 21 days ago)





Time to start again.

More Hillbilly Pumpkin en masse

In testing I have APE221 , PE from a plate pin , PESA Boner rhizo, a plate pin from the main HBP culture


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Offlinenogoodnamesleft
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Re: My journal [Re: nogoodnamesleft]
    #28364584 - 06/18/23 05:33 PM (7 months, 7 days ago)



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Offlinenogoodnamesleft
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Re: My journal [Re: nogoodnamesleft] * 1
    #28364595 - 06/18/23 05:45 PM (7 months, 7 days ago)

Playing with LC



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InvisiblePastywhyteMDiscord
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Re: My journal [Re: nogoodnamesleft]
    #28364758 - 06/18/23 08:48 PM (7 months, 6 days ago)

Those look good


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Offlinenogoodnamesleft
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Re: My journal [Re: Pastywhyte]
    #28371392 - 06/23/23 02:15 PM (7 months, 2 days ago)

Pins


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Offlinenogoodnamesleft
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Re: My journal [Re: nogoodnamesleft]
    #28372469 - 06/24/23 09:38 AM (7 months, 1 day ago)

Hillbilly pumpkin. Might be phasing out this culture but then it’ll do this



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Re: My journal [Re: nogoodnamesleft]
    #28375250 - 06/26/23 12:56 PM (6 months, 30 days ago)

Flushes and stuff



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Offlinenogoodnamesleft
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Re: My journal [Re: Pastywhyte]
    #28375456 - 06/26/23 03:27 PM (6 months, 30 days ago)

Quote:

Pastywhyte said:
Those look good




They all contam because of the lids


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Offlinenogoodnamesleft
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Re: My journal [Re: nogoodnamesleft]
    #28376785 - 06/27/23 04:59 PM (6 months, 29 days ago)

More flushes…



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Re: My journal [Re: nogoodnamesleft]
    #28381666 - 07/01/23 05:46 PM (6 months, 25 days ago)










APE221 performing excellent

Bluey Vuitton needs more testing but probably not a long term candidate


Edited by nogoodnamesleft (07/01/23 05:47 PM)


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Offlinenogoodnamesleft
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Re: My journal [Re: nogoodnamesleft]
    #28395516 - 07/14/23 03:24 PM (6 months, 12 days ago)

Progress and stuff



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Offlinenogoodnamesleft
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Re: My journal [Re: nogoodnamesleft]
    #28401088 - 07/19/23 06:39 PM (6 months, 7 days ago)

Clusters and toadstools





Swabs made from the 100g individual as well as clone plates on PDYA

Cluster was dissected and the center mushroom and largest mushroom were cloned. The largest fruit in the cluster was 74g also done on PDYA

Clones from the 402g fruit are growing out clean and have been transferred. LC will be made in another transfer or two


Edited by nogoodnamesleft (07/19/23 07:44 PM)


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Re: My journal [Re: nogoodnamesleft] * 2
    #28402885 - 07/21/23 01:09 PM (6 months, 5 days ago)

Some specimens







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Re: My journal [Re: nogoodnamesleft] * 2
    #28409617 - 07/26/23 09:05 PM (5 months, 30 days ago)

Really liking the APE culture.

Took a clone from a 250g clustered fruit. Once that tests out, if the results are favorable, I’ll make some swabs and go to spore and select clones again for size and cluster formation







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