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Offlineyas champ
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Re: Long Term Culture Storage On Dehydrated Grain + Revival Tests (moved) [Re: SingularFusion]
    #27543763 - 11/15/21 01:24 AM (2 years, 2 months ago)

I am in Algeria if you know (North Africa), I have already tried to grow the mycelium of agaricus bisporus, but it is always weak (a radius of 2 cm of mycelium only and the color tends to brown) and I wonder if the quality of the mushroom does not play a role in all this, would growing mycelium from a mushroom that has been transplanted several times (several generations) give the same result as growing it directly from the first bag of wheat?
I don't know if I succeeded in making you understand because my English is not good


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InvisibleBaba Yaga
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Re: Long Term Culture Storage On Dehydrated Grain + Revival Tests (moved) [Re: yas champ]
    #27543780 - 11/15/21 02:52 AM (2 years, 2 months ago)

Ok, unfortunately I don't speak your language assuming that french is one of them but there are a few french members on here, I just can't
any names, sorry.

So you are trying to grow edible mushrooms for food? I have the feeling that this thread will not help you much as it is only about a few
experiments that didn't work that well.

If you are growing mushrooms for food then you might be better off asking this question in the gourmet & medicinal forum. Maybe make a
thread with a title like "Looking for someone who speaks french to help me with a few questions."

Here is the link to the gourmet forum:

https://www.shroomery.org/forums/postlist.php/Board/13

Good Luck


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InvisibleBaba Yaga
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Re: Long Term Culture Storage On Dehydrated Grain + Revival Tests (moved) [Re: SingularFusion]
    #27543783 - 11/15/21 03:05 AM (2 years, 2 months ago)

Quote:

Nef said:
I also had trouble reviving things

Was dehydrated tiny (3 x 3mm approx) chips of wood from some woodlover stuff I'm doing

Dehydrated in a filtered little mycobox like what the kits come in, I left it about five months at ambient conditions to dry out and then put to malt yeast agar plates for revival. I poured those a bit hot so there would be some added condensation to wet the chip once it was transferred in

Azure revived but cyan would not, even though you can see mycelial white on the chips, it just won't jump off. Been about six weeks and plates are otherwise spotless. I am keeping them around to see if anything happens but it looks like a fail on revival for me also... Well 50% success rate anyway

I think the method itself has some promise and potential, but there must be a knack to the dehydration that allows revival, something I'm not fully understanding, to make it work right :shrug:

I look forward to seeing what you can figure out in the future Baba






Hey Nef, thanks for popping by and sharing your experience, I always think of wood lover as the once that bounce back from dried up wood
substrates easily.

I am sure that this could work and drying it out to the right moisture content might be the key or freeze drying but all this would be too
much work for me. Maybe I will get back to this if I don't find an alternative.

Anyway, for long term storage I'm trying low nutrient LC out next and I got a few agar wedges in distilled water sitting in the cupboard for
almost a year now.

Will probably wait another year before testing anything.


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Offlineyas champ
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Re: Long Term Culture Storage On Dehydrated Grain + Revival Tests (moved) [Re: Baba Yaga]
    #27543798 - 11/15/21 04:01 AM (2 years, 2 months ago)

I will search in the forum that you suggested, I may find an answer, thank you very much for the answers :rire:.
good luck.


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OfflineSingularFusion
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Re: Long Term Culture Storage On Dehydrated Grain + Revival Tests (moved) [Re: yas champ]
    #27543856 - 11/15/21 06:15 AM (2 years, 2 months ago)

yeah for sure the wedge in distilled water also sounds like it might have some promise

good luck man :rockon:


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Offlineshuna
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Re: Long Term Culture Storage On Dehydrated Grain + Revival Tests (moved) [Re: SingularFusion]
    #27558548 - 11/26/21 03:22 PM (2 years, 2 months ago)

For your long term distilled water test, did you use a wedge of agar and what size, or did you scrape some mycelium off the agar surface?

I'm going to PC some 30ml tubes with say 75% tap water. Then drop say a 5x5mm wedge in and seal at room temp. Seems the easiest way to do it.

Scraping myc off the agar and getting it to stay in the water could be trickier, but then there will be less food for any potential contamination.

Edit: just tried dropping scraped myc to water and it drops easily.

Either way, looking forward to the results.

A grain jar in the fridge is meant to be a good method for long term storage, but bulky and less fridge the better.

:popcorn:


Edited by shuna (11/26/21 04:01 PM)


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InvisibleBaba Yaga
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Re: Long Term Culture Storage On Dehydrated Grain + Revival Tests (moved) [Re: shuna]
    #27558560 - 11/26/21 03:34 PM (2 years, 2 months ago)

Yes, a wedge of agar with a surface area of a stamp roughly.
Tap water should be fine I guess.

Grain in the fridge always turned after a few month in IME and as you
said it's bulky even if you use small jars and will be in the way if you
only have one fridge like me.

Another year and we will know.


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InvisibleJustAPerson
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Re: Long Term Culture Storage On Dehydrated Grain + Revival Tests (moved) [Re: Baba Yaga]
    #27568205 - 12/03/21 08:42 PM (2 years, 1 month ago)

Just mycelium in distilled water works great. I have a 3 year old culture that I fruited out just fine.


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InvisibleBaba Yaga
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Re: Long Term Culture Storage On Dehydrated Grain + Revival Tests (moved) [Re: JustAPerson]
    #27568309 - 12/03/21 10:18 PM (2 years, 1 month ago)

Sounds awesome!

How are you transferring the mycelium?


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InvisibleJustAPerson
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Re: Long Term Culture Storage On Dehydrated Grain + Revival Tests (moved) [Re: Baba Yaga]
    #27568685 - 12/04/21 07:14 AM (2 years, 1 month ago)

Cotton swab


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InvisibleBaba Yaga
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Re: Long Term Culture Storage On Dehydrated Grain + Revival Tests (moved) [Re: JustAPerson]
    #27568696 - 12/04/21 07:30 AM (2 years, 1 month ago)

OK, would you care to explain the process a bit more please.

Swabbing a plate and then swirl the swab in distilled water? You do that more than once?

How much water per container and how much mycelium ends up in each?


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InvisibleJustAPerson
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Re: Long Term Culture Storage On Dehydrated Grain + Revival Tests (moved) [Re: Baba Yaga] * 3
    #27568756 - 12/04/21 08:42 AM (2 years, 1 month ago)

It's a simple process.

1. Using one sterile cotton swab, swab one agar plate and pick up some mycelium
2. Put the swab in sterile water in one sterile culture tube
3. Shake the swab around until the mycelium comes off the swab and stays in the water.
4. Cap the one tube
5. Throw out your swab.
6. Repeat as desired with new tubes and swabs. Reuse the same plate if you want.

You need enough water to fill the culture tube. If you don't fill it all the way to the top you will have the chance that mycelium will float on top of the water and be exposed to air. Does that affect anything? I don't know! But if that does happen, you can try shaking the capped tube to get the mycelium into suspension.

I didn't measure the volume of mycelium per container, because that's hard. Much less than 1 mL, less than the volume of a single droplet of water. The amount of mycelium that gets stuck to a cotton swab. That's how much mycelium ends up per tube.

On the other side of the process

1. Put one sterile cotton swab in one centrifuge tube that has your water-suspended mycelium
2. Shake your swab around until you pick up some mycelium
3. Apply mycelium to agar
4. Toss your swab.
5. If there is mycelium left in your tube, save it, maybe? I've only done single uses.

There's also plenty of room to play with this. Double fist it and use multiple swabs on a single plate at once. Multiple swabs into multiple tubes simultaneously, if you have the dexterity.

Fundamentally, all you care about is getting mycelium into sterile water so that it is not exposed to air or food. However you get there.


Edited by JustAPerson (12/04/21 08:45 AM)


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InvisibleBaba Yaga
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Re: Long Term Culture Storage On Dehydrated Grain + Revival Tests (moved) [Re: JustAPerson]
    #27568984 - 12/04/21 11:46 AM (2 years, 1 month ago)

Thanks for writing this up in detail, using tubes that small is quite appealing. Will try this one day.


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InvisibleJustAPerson
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Re: Long Term Culture Storage On Dehydrated Grain + Revival Tests (moved) [Re: Baba Yaga] * 1
    #27569138 - 12/04/21 01:21 PM (2 years, 1 month ago)

I dug through my order history.

These 5 mL flat bottom tubes are what I used way back when. I selected them because they had a flat bottom. I don't recommend them, though, because they seal with a little o ring between the tube and the screw top, and that is finicky and not great.

Now I use these 15mL centrifuge tubeswith non-flat bottoms. Incrementally more annoying since they don't stand up on their own, but the screw top is way better with no o ring, and a stand is easily attainable or made.

You can also find 2 mL snap top centrifuge tubes. They may work for you, IDK.


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