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OfflineAnamoo
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Registered: 07/29/20
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Last seen: 7 months, 16 days
1st attempt with agar, does this look right?
    #26943121 - 09/18/20 08:49 PM (3 years, 4 months ago)

sup y'all, extremely new to growing mushrooms. I attempted to make agar plates using Patsys no pour tek. In a SAB i squirted a GT spore syringe onto innoc loop and spread across two plates. about 6 days later i start seeing some white growth. being new to this, i don't know if i'm looking at myc or some sort of mold or bacteria. i know the pics are incredibly blurry and ya cant really see much, but i cant get a better shot without taking the cover off.




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Offlinepolyflakes
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Registered: 03/27/08
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Re: 1st attempt with agar, does this look right? [Re: Anamoo]
    #26943133 - 09/18/20 08:58 PM (3 years, 4 months ago)

A little blurry but nothing looks off a lot of condensation but fucking no pours.
You should have the lids wrapped with plastic warp or parafilm. You can get draft between the lid and the container and get contams.


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Offlinepolyflakes
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Re: 1st attempt with agar, does this look right? [Re: polyflakes]
    #26943141 - 09/18/20 09:01 PM (3 years, 4 months ago)

I would take a couple transfers to other plates due to so many growth spots to help differentiate before thay start growing over each other


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Edited by polyflakes (09/18/20 09:02 PM)


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OfflineAnamoo
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Registered: 07/29/20
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Re: 1st attempt with agar, does this look right? [Re: polyflakes]
    #26943150 - 09/18/20 09:06 PM (3 years, 4 months ago)

yes very blurry, i would guess its bacteria given my lack of exp, but we shall see. i just transferred to new plates and wrapped them with seran wrap and put them in a shoebox. fingers crossed...


Edited by Anamoo (09/18/20 09:29 PM)


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OfflineWunFunGai
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Registered: 05/03/16
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Re: 1st attempt with agar, does this look right? [Re: Anamoo]
    #26943259 - 09/18/20 10:21 PM (3 years, 4 months ago)

Hey,

Hard to tell from the pics, but looks like you got some good clumps to xfer from.

I started agar with PastyPlates and never wrapped them and was luck to never had a contam issue. I started my plates from clones I took of some of my most interesting fruits from a PF-TEK BRF MS Spawn I did back in 2016 to take a trip down memory lane of my first grows using the mmgg.html guide when the web was young...lol

If you can get comfortable with PastyPlates, I encourage you to consider the jump to agar and plates, the ability to view things clearly as they are happening is quite enlightening.

I just made the jump to plates and this will probably be my primary method of exploring mycology from here on out. I'll still be using Pasty's for established strains to make pucks to drop into grain jars though because the tek is good and works well for that purpose for me.

Plates will let you transfer stuff out so you can clean up spores to something a bit more sexy:











A semi trace of going from a clone's spores to agar to cleaning up and still working on isolating. Being able to see what's going on helps a lot.

Spawned to grain and waiting for results now...

Enjoy.

WFG :smile:


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OfflinePrincessRosalina
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Registered: 07/22/20
Posts: 83
Last seen: 2 years, 11 months
Re: 1st attempt with agar, does this look right? [Re: Anamoo]
    #26949188 - 09/22/20 11:35 AM (3 years, 4 months ago)

Quote:

Anamoo said:
yes very blurry, i would guess its bacteria given my lack of exp, but we shall see. i just transferred to new plates and wrapped them with seran wrap and put them in a shoebox. fingers crossed...




How is that going?


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