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Invisiblekrock
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Registered: 12/18/19
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Golden Halo, first active grow (currently colonizing oat spawn)
    #26942693 - 09/18/20 03:46 PM (3 years, 4 months ago)

Today I received a Golden Halo print from a generous shroomer!

This will be my first proper active grow.

I have tried earlier with much effort to salvage a badly stored spore syringe solution without any success. You can read about it here. Knowing that I have a legit print makes me very confident that I will be able to grow some cubes this time instead of mold.

I am also excited for the fact that I might grow some mushrooms that have golden spores.

Tomorrow I will streak some Golden Halo spores on a couple of agar plates and get this grow started. Can't wait to tomorrow :laugh:


Edited by krock (04/27/21 12:03 PM)


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Re: Golden Halo Grow (first active grow) [Re: krock]
    #26942724 - 09/18/20 03:58 PM (3 years, 4 months ago)

Excellent!

Golden Halo is one of my favorites. Very aggressive mycelium, plus gold spores. I wish you the best of luck for your first successful active grow  :growshrooms:


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InvisibleJosex
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Re: Golden Halo Grow (first active grow) [Re: One of Us]
    #26942737 - 09/18/20 04:07 PM (3 years, 4 months ago)

:popcorn:


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Re: Golden Halo Grow (first active grow) [Re: Josex]
    #26942801 - 09/18/20 04:42 PM (3 years, 4 months ago)

The gold spores are still fairly rare right?

Is the potency generally normal with these? I know the myc is pretty wild.

I’m also getting ready for a first run, I’ve had it on plates for months now.


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Invisiblekrock
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Re: Golden Halo Grow (first active grow) [Re: A.k.a]
    #26943685 - 09/19/20 08:01 AM (3 years, 4 months ago)

A day has passed and I have now streaked three agar plates!

Here is the print I received:

It's not super yellow/orange gold, but definitely golden brown.


First off, my agar plates are all normal MEA recipe. 10g Malt extract 10g agar agar and 500 ml water. I could have whipped up some softer agar, but I still have 10 plates that were ready to go, so I thought I could try germinating the spores on these plates.

I label my plates as Shimeji to avoid trouble with LE.



So for the first agar plate (A) I tried to scrape the print with my inoculation loop. I couldn't visibly discern whether I got spores on the loop or not. However I still streaked the plate properly like the tek from c10 agar guide, torching the loop between every plate rotation.





For the second plate (B) I scraped the print with a scalpel and put it on the plate. Then I streaked it like the first one. This time I am sure I got spores on it because I could see the agar went a bit darker where I put the spores with my scalpel.




On the third plate my butane torch unfortunately was almost out of juice. So instead of streaking it properly I took two scalpel samples and streaked a simple curved streak from each point and downward. Namely (C) and (D) inoculation points. The last streak I had to flame sterilize my loop with a lighter lol.




And now for some answers:



    Quote:

    Golden Halo is one of my favorites. Very aggressive mycelium, plus gold spores. I wish you the best of luck for your first successful active grow



    Thank you! I look forward to see the mycelium in action :laugh:


    Quote:

    The gold spores are still fairly rare right?



    Yep! The golden spores are not stabilized in this variety so it's like winning the lottery if you actually get golden spores.

    Quote:

    Is the potency generally normal with these? I know the myc is pretty wild.



    As far as I have read, they are the same potency as normal cubes.

    Quote:

    I’m also getting ready for a first run, I’ve had it on plates for months now.



    Cool! Feel free to share pics of your progress :laugh:



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Invisiblekrock
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Re: Golden Halo Grow (first active grow) [Re: krock]
    #26949095 - 09/22/20 10:33 AM (3 years, 4 months ago)

Day 3: Looks like spores on (B) plate are starting to germinate. I can see some tiny translucent mycelium clusters on the plate where I streaked. The brown part of the pic is where I put the initial spores with my scalpel.



There is also a tiny germination on the (CD) plate at the <C> inoculation point. However it was too hard to take a picture of.


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Invisiblekrock
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Re: Golden Halo Grow (first active grow) [Re: krock]
    #26950998 - 09/23/20 12:28 PM (3 years, 4 months ago)

Day 4: More germination and more growth :laugh:

(B) plate


(CD) plate


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Invisiblec10h12n2o
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Re: Golden Halo Grow (first active grow) [Re: krock]
    #26951030 - 09/23/20 12:56 PM (3 years, 4 months ago)

nice! I have some gh projects goin as well



What made you pick GH for your first active? It is notoriously weird and low yielding.  Still a cube tho so hard to fuck up

Any germination on the streak plate yet? Zone 1 should be showing something

The growth on those others doesnt look very clean. Later zones of the streak plate should be better tho if you grab a good lookin colony fast, even if spores are dirty af


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Edited by c10h12n2o (09/23/20 12:58 PM)


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Invisiblekrock
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Re: Golden Halo Grow (first active grow) [Re: c10h12n2o]
    #26951239 - 09/23/20 02:49 PM (3 years, 4 months ago)

Cool! That's some really nice looking prints!

I don't have a good reason for picking Golden Halo as my firstie, I just saw a giveaway and wanted to try it out after I failed cleaning up a extremely contaminated spore solution I had acquired. Maybe I am a bit masochistic, lol. I didn't know GH are weird/low yielding. I never seem to take the easiest route it seems :laugh:


Plate (A) where I tried to scrape spores with my loop has no germination at all. Still empty.

The streak plate where i started with scalpel scrape (B) has what looks to be many early stage germination in zone 1 along the streak line. And also one tiny germination in zone 2, however that one is kind of weird because it is located between two streak lines.

When do you usually see growth in the later zones?

Quote:

The growth on those others doesnt look very clean.



Where do you mean?


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Invisiblec10h12n2o
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Re: Golden Halo Grow (first active grow) [Re: krock]
    #26951286 - 09/23/20 03:12 PM (3 years, 4 months ago)

It might just be the pictures, hard to tell

But based on what im seeing i wouldn't transfer any of that. Still super early tho

When you use a loop, get it red hot then cool it in agar, then scrape spores with it. The agar on the loop will make sure it hrabs plenty of spores

If you want i can send you a print of the most forgiving and best yielding variety ive ever worked with, from a very domesticated line. Its AA+, but after about 5 years of selecting for yields and ffruit size

Even my worst tubs of it yield better than a good tub of anything else

Ive pulled 12.5 dry oz off 1 flush with a particularly good clone

Ofc the print would be ms so who knows. But its basically the opposite of GH: great yields, extremely stable, very domesticated,  gigantic fruits


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Re: Golden Halo Grow (first active grow) [Re: c10h12n2o]
    #26951627 - 09/23/20 06:25 PM (3 years, 4 months ago)

Yeah aa+ was great the couple runs I did. Plus the prints were so heavy I didn’t need a loop. There were big flakes of spores cracking off the print I just pushed one onto a plate with a blade.


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Invisiblekrock
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Re: Golden Halo Grow (first active grow) [Re: c10h12n2o]
    #26952913 - 09/24/20 02:47 PM (3 years, 4 months ago)

With (A) plate I did torch my loop red hot, cooled it in the agar, then tried to scrape. I assume that I didn't scrape hard enough or something. Or maybe my DIY loop is not the best for this task.

I might take you up on your AA+ offer on a later time. Right now I am set on trying to grow these Golden Halos :smile:


Day 5 update:


More growth and what I assume to be mycelium is starting to become more visible.


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Invisiblec10h12n2o
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Re: Golden Halo Grow (first active grow) [Re: krock]
    #26953029 - 09/24/20 04:16 PM (3 years, 4 months ago)

Yeah, both myc and bacteria are more visible  haha

Shoot me a dm if you ever want one of those prints, ill send it over

Looks like the print you have is pretty dirty, youll need to do some serial streakimg and/or well timed transfers to clean it up

Still no germination on plate A?

Remember it doesnt take a ton of spores,  it only takes 2 viable spores to get a dikaryotic culture

Usually when i steak spores zone 1 has visible spore streaks on the agar. If you van see any at all, its plenty (assuming they are viable and havent been microwaved or something)


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Invisiblekrock
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Re: Golden Halo Grow (first active grow) [Re: c10h12n2o]
    #26954560 - 09/25/20 02:12 PM (3 years, 4 months ago)

Quote:

Shoot me a dm if you ever want one of those prints, ill send it over



Thank you! I appreciate it :smile:


Day 6:

Here is the (A) plate, still nothing at all. But the streak pattern is pretty visible. I cooled my loop in the middle.

I am pretty puzzled about that this plate didn't take at all as I thought you needed very little spores to get growth.


Here is (B) plate.


And a close up of zone 1 on (B) plate



I am still not quite sure what to expect from doing spores to agar. As all the actual proper mycelium I have seen are from king oyster clones I have done. And as far as I know, King Oyster mycelium looks much more like mold than cubensis mycelium does.

However I don't think there is bacteria on the plate. To me it looks more like translucent hyphae around the colonies and kind of fluffy mycelium in their center.

Of course this could be wishful thinking, but I read a while back that newly germinated spores often are translucent. I have some post quotes from my journal that I saved:

Quote:

Monokaryotic mycelium from spores will often be transparent. After a week or so you'll see it better as the strands thicken up after it becomes dikaryotic.
RR
source




Quote:

Before you see a dime's worth of growth, filamentous mycelium will already be well on its way as far out as it possibly can. You can transfer from these filaments for faster colonizers. I'm sure in the photograph provided that strands of mycelium not visible to the eye or camera are already there. Check it out with a pocket microscope if you have one they're like 10 bucks.

source





The growth on (CD) plate is pretty similar to the ones I have got on the (B) plate.


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Invisiblec10h12n2o
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Re: Golden Halo Grow (first active grow) [Re: krock]
    #26954700 - 09/25/20 03:43 PM (3 years, 4 months ago)

Nah bro, trust me. Those cloudy discolored patches are 100% bacteria. Theres lots in those pics

You wont accidentally get monokaryotic myc. Spores clump together,  so you pretty much have to use serial dilution and/or surfactants to get that

That zone 2 patch on B might be ok, but i wouldn't transfer any of that, just because id rather have a sure thing than a "its bacterial but i still got some fruits"

Bacterial doesnt mean it wont work, it just isnt ideal

Everything is soooo much easier when you make sure you have a 100% clean culture first


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Invisiblekrock
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Re: Golden Halo Grow (first active grow) [Re: c10h12n2o]
    #26954724 - 09/25/20 04:05 PM (3 years, 4 months ago)

Ah, you mean those brown blobs?

Those are actually just some pieces of vermiculite that stuck on the back of the plate, haha. I'll post a pic without it tomorrow :laugh:


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Re: Golden Halo Grow (first active grow) [Re: krock]
    #26954754 - 09/25/20 04:26 PM (3 years, 4 months ago)

Hahahaha yeah do that, its really hard to tell anything from the pics

Hopefully the discoloration is verm. I dont usually look for verm on agar haha


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Invisiblekrock
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Re: Golden Halo Grow (first active grow) [Re: c10h12n2o]
    #26956092 - 09/26/20 12:56 PM (3 years, 4 months ago)

I removed the verm from the back of the plate. So here is the (B) plate. No bacteria as far as I can see :laugh:

However I still haven't figured out how to properly photograph agar. It's hard to represent how the plates actually look in real life.


Day 7:



What would you recommend transferring? The one growth in zone 2 has grown pretty big now. I don't know if it is because it has more space to grow or it is a better colonizer.


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Invisiblec10h12n2o
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Re: Golden Halo Grow (first active grow) [Re: krock]
    #26956162 - 09/26/20 01:44 PM (3 years, 4 months ago)

Ok cool, yeah that looks better

Yeah its tricky to photograph it and have it look right. Your pics are particularly tricky because it isnt focused on the colony, so even though its well organized its hard to tell if its cloudy vs whispy. Not totally convinced its axenic (only 1 organism)

You could transfer that, itll probably work, but i would rather find a better looking starting point

Maybe itll look better on a new plate

I like redundancy. If it was me i would probably transfer it and streak a few more plates. If it looks good run with it, if not, youll have lots more options to choose from by the time you find out


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Invisiblekrock
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Re: Golden Halo Grow (first active grow) [Re: c10h12n2o]
    #26956274 - 09/26/20 03:13 PM (3 years, 4 months ago)

Thanks for the input on my pictures. I always thought it was good to focus on micro surface details, to be able to see filaments or bacteria on the surface. But I agree it makes more sense to show off the shape of the colonies.
Here is a picture where I tried to focus on the colonies instead:



It does sound like a good idea to do more streak plates. I want to retry scraping spores with my loop anyway. So that I can maybe spread out the growth more than I did this time, so that it also reaches zone 3, 4 and 5. I will aim to get discoloration from the spores in zone 1 with the loop to ensure good spread. 

I am going to transfer that zone 2 growth from (B) plate and let it grow out while the new plates germinate.

When I use up the rest of my current plates (7 left) I want to try switching to some less nutritious agar as it seems to result in better looking mycelium. To this day I have always done 10g malt extract to 500 ml water. But I've seen many people on here using 7g malt extract instead.


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Invisiblec10h12n2o
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Re: Golden Halo Grow (first active grow) [Re: krock]
    #26956366 - 09/26/20 04:13 PM (3 years, 4 months ago)

much better pic

good course of action, redundancy ftw

see how it looks on the next plate

it might be ok, i still dont like the looks of it tho, not where id want to start. looks like more than 1 organism (different types of myc and also the the cloudy growth mingled with the whispy stuff), but might clean up after a few transfers. germ plate growth can be all kinds of ugly

this is why im such a serial streak evangelist. instead of a big pile of whatever spores and contams are on the print, you get a progressively thinner trail of whatever is on the plate, which makes it a lot easier to pick out clean colonies from a dirty print

it gives you tons of options to choose from and often feels like you are starting 10 transfers in on your 1st transfer plate (because colonies from later zones are cleaner and fewer numbers of spore pairings / strains, so less initial sectoring

here is an example of the GH culture i just fruited


this is after 2 transfers, and pinning because it sat unused for too long

GH doesnt always look this crazy rhizo, but it is very common


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Re: Golden Halo Grow (first active grow) [Re: c10h12n2o]
    #26956383 - 09/26/20 04:28 PM (3 years, 4 months ago)

Quote:

c10h12n2o said:





This plate is crazy. Reminds me of a mandala drawing.


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Invisiblec10h12n2o
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Re: Golden Halo Grow (first active grow) [Re: Josex]
    #26956390 - 09/26/20 04:37 PM (3 years, 4 months ago)

i know right! gh can get really wild looking. did you see the substrate it colonized? shit got insane. overlayed the air above the surface haha

that culture is so weird tho because i specifically selected for weirdness.

like i did streak plates and then picked the craziest lookin shit, and thats what i had 2 transfers in


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Re: Golden Halo Grow (first active grow) [Re: c10h12n2o]
    #26957121 - 09/27/20 07:50 AM (3 years, 3 months ago)

Yea I saw it. It was pretty wild. :eek:


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Invisiblekrock
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Re: Golden Halo Grow (first active grow) [Re: Josex]
    #26957490 - 09/27/20 01:48 PM (3 years, 3 months ago)

Wow that plate looks insanely cool! I hope to get something similar!

The cloudy stuff around the colonies are tiny transparent hyphae I think. It looks like that at least. I think maybe the myc is behaving a bit weird because I use full strength agar. I will transfer some on the new half strength plates I made soon. Maybe we can see some interesting differences.

Serial streaking is definitely effective in selecting colonies. Here are some plates where I serial streaked yeast. In the last zones I have single colonies of yeast which is pretty cool:




Day 8:

So since I always do full strength agar (10g LME to 500ml water) I decided it was time to cook up some half strength agar (7.5g LME to 500ml water).

And I did it for two softness degrees: 10g agar agar to 500 ml water and 5g agar agar to 500ml water. The soft agar for germination and the other for transfers.

But I fucked up because I let the agar bottles cool a bit too long so it cooled so much that I could only pour about half of the bottles. And the soft agar I made was in a smaller bottle so the stuff I poured out was really thick and clumpy. Got some pretty ugly plates this time :frown:

Anyways I did a transfer from the growth in zone 2 from the (B) plate to one of my full strength plates. But I didn't do any streaking today, but I will do it soon on those soft (but ugly) plates I made.


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Invisiblekrock
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Re: Golden Halo Grow (first active grow) [Re: krock]
    #26960175 - 09/29/20 11:35 AM (3 years, 3 months ago)

Day 10:

This is where I transferred from. #1 is the grow from zone 2. #2 is from the place with the most growth that I just transferred to see what would happen to it. Don't plan on using it.



These T1 plates have been growing for 2 days. The mycelium looks much better here than on the germ plate in my opinion.


I haven't streaked more plates yet. Will probably do it tomorrow.


Edited by krock (09/29/20 11:38 AM)


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Invisiblekrock
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Re: Golden Halo Grow (first active grow) [Re: krock]
    #26961910 - 09/30/20 12:11 PM (3 years, 3 months ago)

Day 11:

Heres an update on the transfers I did:


Also, I streaked my 4 ugly plates today. (E) plate was so small and ugly that I didn't serial streak it tho.
The other plates I serial streaked as properly as I could. The wavy agar made it harder to streak :laugh:


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Invisiblekrock
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Re: Golden Halo Grow (first active grow) [Re: krock]
    #26963753 - 10/01/20 12:16 PM (3 years, 3 months ago)

Here is the orginal (B) germ plate at day 12:


And here are the two transfers i did at day 4:


The new streak plates haven't done anything yet. But they'll probably do something in a few days.


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Re: Golden Halo Grow (first active grow) [Re: krock]
    #26968220 - 10/03/20 08:53 PM (3 years, 3 months ago)

Nice you are gettin some great growth now

Try to take tiny transfers from the leading edge, as far from that cloudiness as possible

Should have some clean cultures in no time


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Invisiblekrock
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Re: Golden Halo Grow (first active grow) [Re: c10h12n2o]
    #26973768 - 10/07/20 12:43 PM (3 years, 3 months ago)

Wow, it's almost been a week since last update. So this will be a bigger one :laugh:

So first we have the original (B) germ plate, this is day 19 for it:


And here are the transfers I took from it. They are now 10 days old:
(transfer point 1)   (transfer point 2)

I also took transfers from these yesterday. Two transfers from each at approximately the same place. To two plates of agar each, where one is at full strength and the other is at half strength. I'm interested in seeing what makes them different in terms of mycelium growth.


And here we have the new streak plates which are now 7 days old:
(E)
(F)
(G)
(H)

Many of these have very fuzzy growth. Might be because I stored these plates upside down? (to prevent condensation) Or maybe it's because of using half strength and soft agar for the germ plates.

I'm also curious why my serial streaking doesn't seem to work. I can't see any growth in the other zones. I did serial streak all of these except (E) because it didn't fill the plate.


Edited by krock (10/07/20 12:50 PM)


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Invisiblekrock
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Re: Golden Halo Grow (first active grow) [Re: krock]
    #26977439 - 10/09/20 06:27 PM (3 years, 3 months ago)

Time for a new update!

First here are the transfers I took from the (B) plate. 13 days old.


And here are the transfers I took from the plates above. Sample points
are very close to eachother. The growth on the right side is half
strength agar. The growth looks very different and appears almost
bacterial. But on the normal strength plates the growth looks pretty
much like what they did on the first transfer. The plates are 4 days old.


On the streak plates, a lot of the growths have begun growing upwards.
Spiky/fluffy mycelium. It's really hard to capture on photo. But it
looks crazy, which I assume is good for Golden Halo. See center of the pic for what I mean.
Plates are 10 days old.


Any suggestions for what would be good to transfer from these plates?
And I am still wondering what I might've done wrong with my streaking
technique. Because it only seems like zone 1 is growing on the plates.
The other zones are empty.


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Invisiblekrock
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Re: Golden Halo Grow (first active grow) [Re: krock]
    #27007789 - 10/28/20 07:49 AM (3 years, 2 months ago)

So it's been a while...

12 days ago I noticed several of my germ plates started showing rhizo growth at certain spots. So I tried to transfer them by taking small wedges along the rhizo. However after transferring, they haven't shown much rhizo growth, but they look cleaner than the transfers I have done earlier.





5 days ago I noticed another rhizo cluster on a germ plate. This time I decided to just cut out the whole growth and place it on a plate. Unfortunately the wedge split a bit so the growth is scattered a bit around on the plate. But it seems one of the rhizo's have grown down into the plate. I'll try to sample that point. Because I am pretty sure this is a composite of rhizo growth and also other strains.




Two days ago I noticed this germ plate (which is 28 days old) also showing some rhizos. I haven't transferred this yet because I am not really sure what's the best way to transfer this kind of growth when there are other strains under the rhizo.



Edited by krock (10/28/20 07:49 AM)


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Invisiblekrock
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Re: Golden Halo Grow (first active grow) [Re: krock]
    #27064115 - 11/30/20 08:02 AM (3 years, 1 month ago)

OK! 1 month later and here is an update :laugh:

My main goal with this grow is to grow some fruits so that I can go on my first trip.
I have plenty of time so I decided to wait another month and see if any of the germ plates would pin so that I could clone them. So this month I haven't done much besides waiting for the pins and I have been growing some King Oysters meanwhile.

At day 52 or so I got a pin on the side of the F plate:

But it hasn't grown much, so I decided to not clone it, as it might be aborted.

Same F plate at day 62. There are several knots that are starting to elongate. One of them almost looks like a pin now.


G plate hasn't pinned yet, but it seems to have an interesting rhizo growth going on there with lots of knots. I hope one of the knots grows into a pin, because I think this might be good culture.


Finally on the E plate at day 62. I have 3 pins and all of them are pretty recent. So I decided to transfer all of them to one plate each.


I tried to find out how to transfer pins on agar. However no one seems to talk about the proper technique.
I tried transferring the first pin with tweezers, but that didn't work well (maybe because I have indentations on the tip of my tweezers).
However using a scalpel works quite well. Cut the pin at the base and it will stick to the scalpel because you cooled the scalpel in agar before cutting the pin.


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OfflineOne of Us
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Re: Golden Halo Grow (first active grow) [Re: krock]
    #27064117 - 11/30/20 08:05 AM (3 years, 1 month ago)

Wow that G plate myc looks insane!


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OfflineHaywire
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Re: Golden Halo Grow (first active grow) [Re: krock]
    #27064461 - 11/30/20 11:49 AM (3 years, 1 month ago)

Quote:

krock said:






sick edit!


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Invisiblekrock
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Re: Golden Halo Grow (first active grow) [Re: Haywire]
    #27064754 - 11/30/20 02:19 PM (3 years, 1 month ago)

Quote:


One of Us said:
Wow that G plate myc looks insane!




Yeah, I hope it pins so that I am able to capture it!
If it doesn't pin I might just try to transfer one of the knots and see if it grows like this on a different plate. Would be interesting to try out anyhow!

Quote:


Haywire said:
sick edit!




Thanks! My camera has pretty high resolution, but I always need to resize my pics for shroomery to keep within 512 kb limit. So it's nice to show details properly once in a while. The zoom here is what the pictures look like in actual size :smile:


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OfflineHaywire
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Re: Golden Halo Grow (first active grow) [Re: krock]
    #27065692 - 12/01/20 01:41 AM (3 years, 1 month ago)

Quote:

krock said:
The zoom here is what the pictures look like in actual size :smile:






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Ciao mamma, guarda come mi diverto


My grows :mushroom2: Outdoor patches


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Offlinefigjam
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Re: Golden Halo Grow (first active grow) [Re: Haywire]
    #27065696 - 12/01/20 01:50 AM (3 years, 1 month ago)

great progress man. keep up the good work


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Invisiblekrock
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Re: Golden Halo Grow (first active grow) [Re: figjam]
    #27076768 - 12/07/20 09:01 AM (3 years, 1 month ago)

Another week has passed and I have more pins! :mushroom::mushroom::mushroom::mushroom::mushroom:

First off here is one of the pins I transferred 1 week ago. Growth looks pretty organized and balanced.
The small weird looking pieces are just coir in the agar. Because I decided to try out having some coir in the agar recipe to better prepare the shrooms for making enzymes related to breaking down coir.
My room temp is typically 18 C, which probably why it hasn't grown super big in 1 week.


The first transfer I did (72 days ago) from the first germination plate I made is pinning now as well, so I decided to transfer them. 


And the F germination plate (68 days old) has more pins now. On the left there are two pins very close to each other. I hope that means they have cluster genetics. Could potentially make for a good culture.


So in total I now have 8 pins on separate plates. I am looking forward to seeing how they turn out.


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Invisiblekrock
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Re: Golden Halo Grow (first active grow) [Re: krock]
    #27271827 - 03/27/21 12:54 PM (2 years, 9 months ago)

Yo! It's been quite a while... I had a severe case of winter lethargy since last time. So I haven't really done anything since last time.

Until now ----- I am back and have started taking real steps towards a complete grow!!!


So these are the pins I cloned from the germination plates about 3.5 months later.





I have prepared 4 jars of grain spawn and have inoculated each one with a different plate.
The plates I choose to inoculate with are the following:


B1T2P7: This plate has lots of knots and a lot of crazy growth.


FP5: Some knots and seems to have much activity.


EP3: Less activity and knots than the other plates, but seems promising.


EP2: This plate doesn't act all crazy as the other plates do, which I find interesting.

I'm excited to see where these grain jars are going! I have 4 mini-shoeboxes that I want to put each grain jar into with 50/50 coir so that I can test grow them separately. Maybe I'll even get a golden print!


Edited by krock (04/24/21 05:45 AM)


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Invisiblekrock
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Re: Golden Halo Grow (first active grow) [Re: krock]
    #27280380 - 04/24/21 12:11 PM (2 years, 9 months ago)

Hey all, I am so happy that shroomery is back! There really isn't any other place like it.


28 days ago, I put some of my agar plates to grain spawn. I will now show how far my spawn jars have come.

First of all, two of my jars unfortunately contaminated pretty badly. Though they are kind of fighting back. I might try to bury these jars:

They were from these plates:


The other two jars from EP2 and EP3 seem to be clean and haven't stalled.




 


I am unsure if my jars are 100% colonized, but at least it seems to not be that much more left to colonize and on the top side of the grains, mycelium has started going airborne like we often see from golden halo mycelium. I'll let them sit for two more weeks and see if the growth gets any thicker.


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Invisiblekrock
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Posts: 154
Re: Golden Halo Grow (first active grow) [Re: krock]
    #27281058 - 04/25/21 03:42 AM (2 years, 8 months ago)

I've been researching a bit and it turns out my jars do not look like 100% colonized spawn. Even though they have covered all the grains with some mycelium, the spawn isn't completely covered in thick white mycelium like the images of spawn I've seen on here.

As part of my research I read the Clean spawn checklist for the new grower by p9hu7 and it suggest that shaking the spawn can help identify potential contamination in the spawn.

So I decided to shake the EP3 jar and see if that helps. I'll keep the other jar unshaken as a comparison.

Fortunately shaking the jar was easy (much easier than King Oyster spawn), so now I'll wait 3-4 days and see if the jar recovers nicely.


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Invisiblekrock
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Posts: 154
Re: Golden Halo Grow (first active grow) [Re: krock]
    #27283935 - 04/27/21 01:47 PM (2 years, 8 months ago)

The jar I shook is recovering nicely 2 days later:


The other jar seems to have grown a bit more, so it seems to not have stalled, which I am glad for. Here is the jar 2 days ago and the jar today side by side. - I managed to take the picture from almost the same angle, so it's kinda fun to switch between them. If you look closely, the aerial myc has grown taller and there are some more knots.


Please tell me if you see anything that seems off on these jars.
I might be fooling myself thinking they are going better than they actually are :smile:


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