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c10h12n2o
serial dilutor



Registered: 01/21/15
Posts: 3,200
Loc: the abyss
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Re: Golden Halo Grow (first active grow) [Re: krock]
#26956366 - 09/26/20 04:13 PM (3 years, 4 months ago) |
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much better pic
good course of action, redundancy ftw
see how it looks on the next plate
it might be ok, i still dont like the looks of it tho, not where id want to start. looks like more than 1 organism (different types of myc and also the the cloudy growth mingled with the whispy stuff), but might clean up after a few transfers. germ plate growth can be all kinds of ugly
this is why im such a serial streak evangelist. instead of a big pile of whatever spores and contams are on the print, you get a progressively thinner trail of whatever is on the plate, which makes it a lot easier to pick out clean colonies from a dirty print
it gives you tons of options to choose from and often feels like you are starting 10 transfers in on your 1st transfer plate (because colonies from later zones are cleaner and fewer numbers of spore pairings / strains, so less initial sectoring
here is an example of the GH culture i just fruited

this is after 2 transfers, and pinning because it sat unused for too long
GH doesnt always look this crazy rhizo, but it is very common
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  C10's Agar Guide + Tips and Tricks | c10's Flow Hood Build Guide "Partial knowledge is more triumphant than complete knowledge; it takes things to be simpler than they are, and so makes its theory more popular and convincing." "Convictions are more dangerous enemies of truth than lies" ― Friedrich Nietzsche
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Josex
#cheat_code


Registered: 11/13/15
Posts: 8,995
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Re: Golden Halo Grow (first active grow) [Re: c10h12n2o]
#26956383 - 09/26/20 04:28 PM (3 years, 4 months ago) |
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Quote:
c10h12n2o said:

This plate is crazy. Reminds me of a mandala drawing.
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c10h12n2o
serial dilutor



Registered: 01/21/15
Posts: 3,200
Loc: the abyss
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Re: Golden Halo Grow (first active grow) [Re: Josex]
#26956390 - 09/26/20 04:37 PM (3 years, 4 months ago) |
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i know right! gh can get really wild looking. did you see the substrate it colonized? shit got insane. overlayed the air above the surface haha
that culture is so weird tho because i specifically selected for weirdness.
like i did streak plates and then picked the craziest lookin shit, and thats what i had 2 transfers in
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  C10's Agar Guide + Tips and Tricks | c10's Flow Hood Build Guide "Partial knowledge is more triumphant than complete knowledge; it takes things to be simpler than they are, and so makes its theory more popular and convincing." "Convictions are more dangerous enemies of truth than lies" ― Friedrich Nietzsche
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Josex
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Re: Golden Halo Grow (first active grow) [Re: c10h12n2o]
#26957121 - 09/27/20 07:50 AM (3 years, 3 months ago) |
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Yea I saw it. It was pretty wild.
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krock
Krockodile

Registered: 12/18/19
Posts: 154
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Re: Golden Halo Grow (first active grow) [Re: Josex]
#26957490 - 09/27/20 01:48 PM (3 years, 3 months ago) |
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Wow that plate looks insanely cool! I hope to get something similar!
The cloudy stuff around the colonies are tiny transparent hyphae I think. It looks like that at least. I think maybe the myc is behaving a bit weird because I use full strength agar. I will transfer some on the new half strength plates I made soon. Maybe we can see some interesting differences.
Serial streaking is definitely effective in selecting colonies. Here are some plates where I serial streaked yeast. In the last zones I have single colonies of yeast which is pretty cool:

Day 8:
So since I always do full strength agar (10g LME to 500ml water) I decided it was time to cook up some half strength agar (7.5g LME to 500ml water).
And I did it for two softness degrees: 10g agar agar to 500 ml water and 5g agar agar to 500ml water. The soft agar for germination and the other for transfers.
But I fucked up because I let the agar bottles cool a bit too long so it cooled so much that I could only pour about half of the bottles. And the soft agar I made was in a smaller bottle so the stuff I poured out was really thick and clumpy. Got some pretty ugly plates this time 
Anyways I did a transfer from the growth in zone 2 from the (B) plate to one of my full strength plates. But I didn't do any streaking today, but I will do it soon on those soft (but ugly) plates I made.
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krock
Krockodile

Registered: 12/18/19
Posts: 154
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Re: Golden Halo Grow (first active grow) [Re: krock]
#26960175 - 09/29/20 11:35 AM (3 years, 3 months ago) |
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Day 10:
This is where I transferred from. #1 is the grow from zone 2. #2 is from the place with the most growth that I just transferred to see what would happen to it. Don't plan on using it.
These T1 plates have been growing for 2 days. The mycelium looks much better here than on the germ plate in my opinion.

I haven't streaked more plates yet. Will probably do it tomorrow.
Edited by krock (09/29/20 11:38 AM)
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krock
Krockodile

Registered: 12/18/19
Posts: 154
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Re: Golden Halo Grow (first active grow) [Re: krock]
#26961910 - 09/30/20 12:11 PM (3 years, 3 months ago) |
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Day 11:
Heres an update on the transfers I did:

Also, I streaked my 4 ugly plates today. (E) plate was so small and ugly that I didn't serial streak it tho. The other plates I serial streaked as properly as I could. The wavy agar made it harder to streak 
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krock
Krockodile

Registered: 12/18/19
Posts: 154
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Re: Golden Halo Grow (first active grow) [Re: krock]
#26963753 - 10/01/20 12:16 PM (3 years, 3 months ago) |
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Here is the orginal (B) germ plate at day 12:

And here are the two transfers i did at day 4:

The new streak plates haven't done anything yet. But they'll probably do something in a few days.
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c10h12n2o
serial dilutor



Registered: 01/21/15
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Re: Golden Halo Grow (first active grow) [Re: krock]
#26968220 - 10/03/20 08:53 PM (3 years, 3 months ago) |
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Nice you are gettin some great growth now
Try to take tiny transfers from the leading edge, as far from that cloudiness as possible
Should have some clean cultures in no time
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  C10's Agar Guide + Tips and Tricks | c10's Flow Hood Build Guide "Partial knowledge is more triumphant than complete knowledge; it takes things to be simpler than they are, and so makes its theory more popular and convincing." "Convictions are more dangerous enemies of truth than lies" ― Friedrich Nietzsche
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krock
Krockodile

Registered: 12/18/19
Posts: 154
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Re: Golden Halo Grow (first active grow) [Re: c10h12n2o]
#26973768 - 10/07/20 12:43 PM (3 years, 3 months ago) |
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Wow, it's almost been a week since last update. So this will be a bigger one 
So first we have the original (B) germ plate, this is day 19 for it:

And here are the transfers I took from it. They are now 10 days old: (transfer point 1) (transfer point 2) 
I also took transfers from these yesterday. Two transfers from each at approximately the same place. To two plates of agar each, where one is at full strength and the other is at half strength. I'm interested in seeing what makes them different in terms of mycelium growth.
And here we have the new streak plates which are now 7 days old: (E) (F) (G) (H) 
Many of these have very fuzzy growth. Might be because I stored these plates upside down? (to prevent condensation) Or maybe it's because of using half strength and soft agar for the germ plates.
I'm also curious why my serial streaking doesn't seem to work. I can't see any growth in the other zones. I did serial streak all of these except (E) because it didn't fill the plate.
Edited by krock (10/07/20 12:50 PM)
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krock
Krockodile

Registered: 12/18/19
Posts: 154
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Re: Golden Halo Grow (first active grow) [Re: krock]
#26977439 - 10/09/20 06:27 PM (3 years, 3 months ago) |
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Time for a new update!
First here are the transfers I took from the (B) plate. 13 days old.
And here are the transfers I took from the plates above. Sample points are very close to eachother. The growth on the right side is half strength agar. The growth looks very different and appears almost bacterial. But on the normal strength plates the growth looks pretty much like what they did on the first transfer. The plates are 4 days old.
On the streak plates, a lot of the growths have begun growing upwards. Spiky/fluffy mycelium. It's really hard to capture on photo. But it looks crazy, which I assume is good for Golden Halo. See center of the pic for what I mean. Plates are 10 days old.

Any suggestions for what would be good to transfer from these plates? And I am still wondering what I might've done wrong with my streaking technique. Because it only seems like zone 1 is growing on the plates. The other zones are empty.
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krock
Krockodile

Registered: 12/18/19
Posts: 154
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Re: Golden Halo Grow (first active grow) [Re: krock]
#27007789 - 10/28/20 07:49 AM (3 years, 2 months ago) |
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So it's been a while...
12 days ago I noticed several of my germ plates started showing rhizo growth at certain spots. So I tried to transfer them by taking small wedges along the rhizo. However after transferring, they haven't shown much rhizo growth, but they look cleaner than the transfers I have done earlier.
5 days ago I noticed another rhizo cluster on a germ plate. This time I decided to just cut out the whole growth and place it on a plate. Unfortunately the wedge split a bit so the growth is scattered a bit around on the plate. But it seems one of the rhizo's have grown down into the plate. I'll try to sample that point. Because I am pretty sure this is a composite of rhizo growth and also other strains.

Two days ago I noticed this germ plate (which is 28 days old) also showing some rhizos. I haven't transferred this yet because I am not really sure what's the best way to transfer this kind of growth when there are other strains under the rhizo.
Edited by krock (10/28/20 07:49 AM)
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krock
Krockodile

Registered: 12/18/19
Posts: 154
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Re: Golden Halo Grow (first active grow) [Re: krock]
#27064115 - 11/30/20 08:02 AM (3 years, 1 month ago) |
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OK! 1 month later and here is an update 
My main goal with this grow is to grow some fruits so that I can go on my first trip. I have plenty of time so I decided to wait another month and see if any of the germ plates would pin so that I could clone them. So this month I haven't done much besides waiting for the pins and I have been growing some King Oysters meanwhile. 
At day 52 or so I got a pin on the side of the F plate:
 But it hasn't grown much, so I decided to not clone it, as it might be aborted.
Same F plate at day 62. There are several knots that are starting to elongate. One of them almost looks like a pin now.

G plate hasn't pinned yet, but it seems to have an interesting rhizo growth going on there with lots of knots. I hope one of the knots grows into a pin, because I think this might be good culture.

Finally on the E plate at day 62. I have 3 pins and all of them are pretty recent. So I decided to transfer all of them to one plate each.
I tried to find out how to transfer pins on agar. However no one seems to talk about the proper technique. I tried transferring the first pin with tweezers, but that didn't work well (maybe because I have indentations on the tip of my tweezers). However using a scalpel works quite well. Cut the pin at the base and it will stick to the scalpel because you cooled the scalpel in agar before cutting the pin.
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One of Us
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Re: Golden Halo Grow (first active grow) [Re: krock]
#27064117 - 11/30/20 08:05 AM (3 years, 1 month ago) |
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Wow that G plate myc looks insane!
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Haywire
Wetspot Wizard



Registered: 12/29/13
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Re: Golden Halo Grow (first active grow) [Re: krock]
#27064461 - 11/30/20 11:49 AM (3 years, 1 month ago) |
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Quote:
krock said:

sick edit!
-------------------- Ciao mamma, guarda come mi diverto My grows Outdoor patches
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krock
Krockodile

Registered: 12/18/19
Posts: 154
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Re: Golden Halo Grow (first active grow) [Re: Haywire]
#27064754 - 11/30/20 02:19 PM (3 years, 1 month ago) |
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Quote:
One of Us said: Wow that G plate myc looks insane!
Yeah, I hope it pins so that I am able to capture it! If it doesn't pin I might just try to transfer one of the knots and see if it grows like this on a different plate. Would be interesting to try out anyhow!
Quote:
Haywire said: sick edit!
Thanks! My camera has pretty high resolution, but I always need to resize my pics for shroomery to keep within 512 kb limit. So it's nice to show details properly once in a while. The zoom here is what the pictures look like in actual size
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Haywire
Wetspot Wizard



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Re: Golden Halo Grow (first active grow) [Re: krock]
#27065692 - 12/01/20 01:41 AM (3 years, 1 month ago) |
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Quote:
krock said: The zoom here is what the pictures look like in actual size 
-------------------- Ciao mamma, guarda come mi diverto My grows Outdoor patches
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figjam
Random Hero


Registered: 06/23/20
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Re: Golden Halo Grow (first active grow) [Re: Haywire]
#27065696 - 12/01/20 01:50 AM (3 years, 1 month ago) |
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great progress man. keep up the good work
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krock
Krockodile

Registered: 12/18/19
Posts: 154
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Re: Golden Halo Grow (first active grow) [Re: figjam]
#27076768 - 12/07/20 09:01 AM (3 years, 1 month ago) |
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Another week has passed and I have more pins!     
First off here is one of the pins I transferred 1 week ago. Growth looks pretty organized and balanced. The small weird looking pieces are just coir in the agar. Because I decided to try out having some coir in the agar recipe to better prepare the shrooms for making enzymes related to breaking down coir. My room temp is typically 18 C, which probably why it hasn't grown super big in 1 week.
The first transfer I did (72 days ago) from the first germination plate I made is pinning now as well, so I decided to transfer them.
And the F germination plate (68 days old) has more pins now. On the left there are two pins very close to each other. I hope that means they have cluster genetics. Could potentially make for a good culture.

So in total I now have 8 pins on separate plates. I am looking forward to seeing how they turn out.
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krock
Krockodile

Registered: 12/18/19
Posts: 154
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Re: Golden Halo Grow (first active grow) [Re: krock]
#27271827 - 03/27/21 12:54 PM (2 years, 9 months ago) |
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Yo! It's been quite a while... I had a severe case of winter lethargy since last time. So I haven't really done anything since last time.
Until now ----- I am back and have started taking real steps towards a complete grow!!!
So these are the pins I cloned from the germination plates about 3.5 months later.
 
I have prepared 4 jars of grain spawn and have inoculated each one with a different plate. The plates I choose to inoculate with are the following:
 B1T2P7: This plate has lots of knots and a lot of crazy growth.
 FP5: Some knots and seems to have much activity.
EP3: Less activity and knots than the other plates, but seems promising.
EP2: This plate doesn't act all crazy as the other plates do, which I find interesting.
I'm excited to see where these grain jars are going! I have 4 mini-shoeboxes that I want to put each grain jar into with 50/50 coir so that I can test grow them separately. Maybe I'll even get a golden print!
Edited by krock (04/24/21 05:45 AM)
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