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Offlinemeowjinx
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What Kind of Broken Glass Should I Use to Agitate LC?
    #26939901 - 09/16/20 10:54 PM (3 years, 4 months ago)

Apologies if there's already a TEK for this or if it's a dumb question, I did try using the search function but found no clear answer

I'm gearing up to try to make my first ever LC. I have the lid components: vacutainers for SHIPs, Whatman filters on the way, large syringes, RTV silicone, etc.

But one thing I've seen in most LC teks is that it helps to agitate the myc. I considered going w/ a magnetic stirrer, but it seems that high-quality ones either require electrical know-how (I'm not a very handy person) or big bucks. So the cheap, easy solutions are marbles or broken glass. I figured marbles would be more likely to damage the mason jars if I swirl too vigorously, so I'm thinking of going w/ broken glass


What type of glass works best? How big or small should I make the glass fragments for optimal agitation? How many pieces should I put in each half-pint jar?


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Offlineblindsighted
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Re: What Kind of Broken Glass Should I Use to Agitate LC? [Re: meowjinx]
    #26939941 - 09/17/20 12:05 AM (3 years, 4 months ago)

Marble is fine


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Offlinemeowjinx
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Re: What Kind of Broken Glass Should I Use to Agitate LC? [Re: blindsighted]
    #26939976 - 09/17/20 12:54 AM (3 years, 4 months ago)

Quote:

blindsighted said:
Marble is fine




Thank you for the input. I suppose I can go ahead and try it with marbles, see how that goes


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OfflineYeetusdeetus
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Re: What Kind of Broken Glass Should I Use to Agitate LC? [Re: meowjinx] * 1
    #26940748 - 09/17/20 01:46 PM (3 years, 4 months ago)

I use glass from broken bottles, just make sure to clean them before you break them lol

You can find decent deals on used stir plates if you look around. I’d look for something from Corning or Ika


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Offlinemeowjinx
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Re: What Kind of Broken Glass Should I Use to Agitate LC? [Re: Yeetusdeetus]
    #26940781 - 09/17/20 02:06 PM (3 years, 4 months ago)

Quote:

Yeetusdeetus said:
I use glass from broken bottles, just make sure to clean them before you break them lol

You can find decent deals on used stir plates if you look around. I’d look for something from Corning or Ika




Thanks for the tip! Yeah, clean and then PC them was my plan lol. I do have an empty bottle of Everclear that I'd been using as an SAB weight


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OfflineYeetusdeetus
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Re: What Kind of Broken Glass Should I Use to Agitate LC? [Re: meowjinx] * 1
    #26940801 - 09/17/20 02:20 PM (3 years, 4 months ago)

That label glue can be tricky haha just don’t cut yourself :gooby:

I keep my old scalpel blades in an empty peanut container and I’ll toss 5 or 6 in there if I don’t have any bottles around


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Offlinemeowjinx
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Re: What Kind of Broken Glass Should I Use to Agitate LC? [Re: Yeetusdeetus]
    #26940809 - 09/17/20 02:25 PM (3 years, 4 months ago)

Quote:

Yeetusdeetus said:
That label glue can be tricky haha just don’t cut yourself :gooby:

I keep my old scalpel blades in an empty peanut container and I’ll toss 5 or 6 in there if I don’t have any bottles around




Huh, never thought to use scalpel blades. That's also a good idea. Plus they couldn't get sucked into the syringe like glass can. I have hundreds of replacement X-Acto blades so I could def do this. Thanks!


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InvisibleLotKid
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Re: What Kind of Broken Glass Should I Use to Agitate LC? [Re: meowjinx]
    #26941740 - 09/18/20 05:37 AM (3 years, 4 months ago)

Sclapel blades arent heavy tho. I've thrown a nickel in to help stir. Worked fine.


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InvisiblebodhisattaMDiscordReddit
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Re: What Kind of Broken Glass Should I Use to Agitate LC? [Re: LotKid]
    #26941759 - 09/18/20 05:55 AM (3 years, 4 months ago)

Scalpel blades also will just rust.


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InvisibleJosex
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Re: What Kind of Broken Glass Should I Use to Agitate LC? [Re: bodhisatta] * 1
    #26941772 - 09/18/20 06:06 AM (3 years, 4 months ago)



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InvisibleJosex
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Re: What Kind of Broken Glass Should I Use to Agitate LC? [Re: Josex]
    #26941777 - 09/18/20 06:13 AM (3 years, 4 months ago)



Some old pics. Great times.


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InvisibleLotKid
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Re: What Kind of Broken Glass Should I Use to Agitate LC? [Re: Josex]
    #26941804 - 09/18/20 06:31 AM (3 years, 4 months ago)

Thats the best option for hand shaking i've seen yet.


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InvisibleJosex
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Re: What Kind of Broken Glass Should I Use to Agitate LC? [Re: LotKid]
    #26941824 - 09/18/20 06:50 AM (3 years, 4 months ago)

Those came with some blender bottles I bought at the time. I didn't know they also sell these things separately for cheap until just now that I did a quick search on Aliexpress to help OP out.


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OfflineYeetusdeetus
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Re: What Kind of Broken Glass Should I Use to Agitate LC? [Re: Josex]
    #26941869 - 09/18/20 07:29 AM (3 years, 4 months ago)

Here I am thinking I’m hot shit recycling cult materials and here come some TCs to shit on my brain baby :lol:. Rust hasn’t really been a big issue with my lcs but that could just be dumb luck.

I think I remember aka saying that sometimes he doesn’t even use something in the lc to stir. He swirls until it’s spinning good and just gives it a quick slosh in the other direction to help break it up


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InvisiblebodhisattaMDiscordReddit
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Re: What Kind of Broken Glass Should I Use to Agitate LC? [Re: Yeetusdeetus]
    #26941895 - 09/18/20 07:46 AM (3 years, 4 months ago)

Pretty much everyone over thinks it. Anything works well to agitate LC


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InvisibleLotKid
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Re: What Kind of Broken Glass Should I Use to Agitate LC? [Re: Yeetusdeetus]
    #26941898 - 09/18/20 07:47 AM (3 years, 4 months ago)

I used to use lc all the time. I rarely make it anymore. Whatya yall think a dem apples?

https://www.shroomery.org/forums/showflat.php/Number/26941820#26941820


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Offlineverum subsequentis
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Re: What Kind of Broken Glass Should I Use to Agitate LC? [Re: LotKid]
    #26941945 - 09/18/20 08:14 AM (3 years, 4 months ago)

Me rikey dem apples, except for that one.

I've used all kinds of things in LCs. My favorite is just keeping them on a stir plate but if i don't have plate space available i just throw in a marble. So long as you don't let them grow to long without a swirl the marble will do just fine.

I shy away from broken glass because it doesn't work as well as a marble and i don't like the idea of having little shards of broken glass possibly making their way into my spawn bags.

Josex, those things seem perfect.


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InvisibleJosex
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Re: What Kind of Broken Glass Should I Use to Agitate LC? [Re: LotKid]
    #26941946 - 09/18/20 08:15 AM (3 years, 4 months ago)

Quote:

LotKid said:
I used to use lc all the time. I rarely make it anymore. Whatya yall think a dem apples?

https://www.shroomery.org/forums/showflat.php/Number/26941820#26941820




I totally get where you're coming from, G2G is the way to go for you and your freaking shroom factory :lol:. To me LC is not an extra step at all though, LC is the step and agar is totally redundant (let me explain).

I used to be obsessed with agar and would spend countless hours transferring and culturing just for the fuck of it, but I got fed up with it eventually, realized I couldn't keep up with the agar workload I needlessly imposed upon myself (no matter how many times I tried to keep the workload down, it always ended up getting crazy af) and became more and more lazy over time to the point I realized I wanted to spend as little time doing culture work as possible. So I made the switch to a totally unorthodox way of doing things.
I only work with these pucks now (pf tek sub).

They take a long ass time to get 100% colonized (a month or so if I knock them via biopsy), so when it's time to transfer I just needle-poke it and transfer to a blank puck. I confess I make my LC's out of these pucks.
:shamecube:

It really doesn't feel like work at all and even though I can understand this can be viewed as dicey by many, I can assure you is a pretty solid way of doing things. Plus them pucks give me some killer clones from time to time. :grin:


Edited by Josex (09/18/20 11:49 AM)


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Offlinemeowjinx
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Re: What Kind of Broken Glass Should I Use to Agitate LC? [Re: LotKid]
    #26941947 - 09/18/20 08:15 AM (3 years, 4 months ago)

Quote:

LotKid said:
Sclapel blades arent heavy tho. I've thrown a nickel in to help stir. Worked fine.





Quote:

bodhisatta said:
Pretty much everyone over thinks it. Anything works well to agitate LC




Gotcha. Thanks fellas.

Quote:

Josex said:
Here, here!

:cookiemonster:  :elmo:




Oh, right. I'd read about this idea, too. They look awesome in your pics. I thought they might be too big to roll around properly in my half pints, but I'm pretty sure I can acquire one of these from a cheap shaker cup and test it out. Thanks!

Quote:

Yeetusdeetus said:
Here I am thinking I’m hot shit recycling cult materials and here come some TCs to shit on my brain baby :lol:. Rust hasn’t really been a big issue with my lcs but that could just be dumb luck.

I think I remember aka saying that sometimes he doesn’t even use something in the lc to stir. He swirls until it’s spinning good and just gives it a quick slosh in the other direction to help break it up




Honestly, it sounded like a good idea to me lol. I'm gonna be using Pasty's EzLC tek, which I'm thinking might be less viscous than honey or syrup recipes, so maybe it WOULD work despite their weight. Didn't think of them rusting, tho


Quote:

LotKid said:
I used to use lc all the time. I rarely make it anymore. Whatya yall think a dem apples?

https://www.shroomery.org/forums/showflat.php/Number/26941820#26941820




Got DAMN. That's almost x20 per jar. I had heard about your ability to stretch spawn. Impressive. Honestly, I do prefer G2G as I've done it before and feel more comfortable doing it. It's just that doing G2G w/ 40+ jars can be hella cumbersome w/ only my SAB. My plan is to do LC > 10-20 jars > G2G one quart jar per bag. Definitely risky, w/ some contam vectors, but w/ the space available to me at the moment it seems like the most viable way to do this kind of bulk. Honestly, I just do not have the Godly patience required to load 40+ jars in and out of my SAB for straight up G2G


Edited by meowjinx (09/18/20 08:25 AM)


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OfflineYeetusdeetus
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Re: What Kind of Broken Glass Should I Use to Agitate LC? [Re: meowjinx]
    #26941991 - 09/18/20 08:35 AM (3 years, 4 months ago)

I don’t think I’d be able to manage having a jar open long enough to knock up 39 more without contaminating it. Even if the lid was put back on every couple jars I bet my hands would get all sweaty and I’d end up breaking something haha.

Don’t you need some special certs or something to sell spawn? I’ve thought about setting up a small oyster grow to sell to my brother in laws farm/produce stand. I’d much rather just make the spawn but I think the laws are a little more lax for mushrooms since they’re treated like veggies.


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