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Offlineraoulduke69
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Registered: 07/12/20
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Agar - Should Heated Loop Penetrate Agar When Cooling?
    #26939777 - 09/16/20 08:53 PM (3 years, 4 months ago)

I recently did some pasty plates. I know you have to dip the heated loop into the agar to cool. But it seemed I was having difficulty getting the loop inside the agar. It seemed like I was just barely scraping the surface. I might attribute this to the agar recipe I used producing something too stiff, or to the fact that maybe I needed a thicker layer of agar in the plate (I was just barely covering the bottom with a thin layer).

Any thoughts?

Follow up question: when sterilizing pasty plates, should the foil cover the bottom of the plate as well? This was hard to tell in the photos in the tek. Coming from doing PF tek, I'm used to just wrapping the lid in foil.

Thanks.


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