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OnlineI AM SWIM
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Black spots
    #26939441 - 09/16/20 05:02 PM (3 years, 4 months ago)

One of my jars has some black spots. I'm going to isolate it and spawn it separate from the rest of my grain
jars. I'm wondering what they are? I highly doubt it's a contam, but I'm wondering what you all think.







The rest of my jars are fine, however, the black spots appeared after full colonization.


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Offlinejunk_f00d
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Re: Black spots [Re: I AM SWIM]
    #26939445 - 09/16/20 05:04 PM (3 years, 4 months ago)

Are these cubensis? If so, it is certainly a contam, and I would not recommend trying to 'isolate it and spawn it'.


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Re: Black spots [Re: I AM SWIM]
    #26939450 - 09/16/20 05:06 PM (3 years, 4 months ago)

why do you highly doubt this being contam? just curious


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Re: Black spots [Re: evlyshrooms]
    #26939478 - 09/16/20 05:18 PM (3 years, 4 months ago)

That whole jar is a mess. Get rid of it.


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Re: Black spots [Re: evlyshrooms]
    #26939490 - 09/16/20 05:22 PM (3 years, 4 months ago)

Quote:

evlyshrooms said:
why do you highly doubt this being contam? just curious




Just because I feel like I've ran into them in the past.

Now I'm certain it's a contam, the jar smells like ammonia or something.


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Re: Black spots [Re: junk_f00d]
    #26939494 - 09/16/20 05:23 PM (3 years, 4 months ago)

Quote:

junk_f00d said:
Are these cubensis? If so, it is certainly a contam, and I would not recommend trying to 'isolate it and spawn it'.




Yes, cubes.
Ecuador.


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Re: Black spots [Re: Mycolorado]
    #26939498 - 09/16/20 05:25 PM (3 years, 4 months ago)

Quote:

Mycolorado said:
That whole jar is a mess. Get rid of it.




What causes these issues? Sterile technique?


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Re: Black spots [Re: I AM SWIM]
    #26939507 - 09/16/20 05:28 PM (3 years, 4 months ago)

Failure at any given step for any given jar. Improper grain prep, sterile technique, or equipment.


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Re: Black spots [Re: I AM SWIM]
    #26939511 - 09/16/20 05:30 PM (3 years, 4 months ago)

Or lack there of, more precisely.  On top of whatever the black is, it has the “wet ice cream” look indicative of bacterial contamination and the fact that it’s not colonizing all the way is another indicator of bacteria.  How’d you noc it?  Either way, shit happens, even when using seemingly clean cultures on agar.  Good technique come with practice.

And what junk food said.


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Edited by Mycolorado (09/16/20 05:31 PM)


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InvisibleRoger Clemency
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Re: Black spots [Re: Mycolorado]
    #26939515 - 09/16/20 05:33 PM (3 years, 4 months ago)

Can you post pictures with regular light? I’m curious to see what’s going on in there.


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Re: Black spots [Re: Mycolorado]
    #26939543 - 09/16/20 05:50 PM (3 years, 4 months ago)

Quote:

Mycolorado said:
Or lack there of, more precisely.  On top of whatever the black is, it has the “wet ice cream” look indicative of bacterial contamination and the fact that it’s not colonizing all the way is another indicator of bacteria.  How’d you noc it?  Either way, shit happens, even when using seemingly clean cultures on agar.  Good technique come with practice.

And what junk food said.




I inoculated it with an Agar wedge.

Quote:

Roger Clemency said:
Can you post pictures with regular light? I’m curious to see what’s going on in there.




I just tossed it! D:
If I run into the same issue I'll post a picture with regular lighting and send you a PM.

Quote:

junk_f00d said:
Failure at any given step for any given jar. Improper grain prep, sterile technique, or equipment.



QFT

I never shook that jar and I'm wondering if not shaking it could be the cause or somehow play a role for bacterial contamination.


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Re: Black spots [Re: I AM SWIM]
    #26939569 - 09/16/20 05:59 PM (3 years, 4 months ago)

Not shaking has nothing to do with contamination.  It got in there through poor technique, contaminated culture, faulty filter or incomplete sterilization of the grain.


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Re: Black spots [Re: I AM SWIM]
    #26939630 - 09/16/20 06:35 PM (3 years, 4 months ago)

Quote:

I AM SWIM said:
I never shook that jar and I'm wondering if not shaking it could be the cause or somehow play a role for bacterial contamination.




Not shaking it may have slowed colonization, which can give bacteria a longer time to get a strong foothold in the untouched areas. Especially if you did something like not shaking after a G2G and had an 'frosting layer' on the top and that's it. But the bacteria was already present, and that's the real problem of course.


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