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OfflineRuaridh McD
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Enokitake Liquid Culture
    #26938807 - 09/16/20 10:55 AM (3 years, 4 months ago)

Hello all iv just started cultivating different gourmet and im trying to understand every step in the process. I have managed to grow some liquid culture from a few different species that I can buy locally, oysters for instance and I was wondering if anyone has managed to grow liquid culture from some Enokitake mushrooms and if you had any advice on doing it. I have just used the technique of scooping a piece from inside the mushroom with a syringe but I think that the individual Enokitake mushrooms are too small to do this with. Any advice much apreciated.

Cheers Ru


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OfflineForresterM
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Re: Enokitake Liquid Culture [Re: Ruaridh McD]
    #26939390 - 09/16/20 04:26 PM (3 years, 4 months ago)

You will want to go from mushroom tissue ===> agar ===> liquid culture.  Don't go straight to liquid culture it will be contaminated.  As far as how to get it out of the mushroom, that may be tough.  Another reason you will want agar, to separate out the mycelium from the contamination that will come along with it.

Are you familiar with agar work at all?  If not, you might want to do some research!


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Repugnant is a creature who would squander the ability to lift an eye to heaven, conscious of his fleeting time here.
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Have some medicinal mushrooms and want to get the most out of them?  Try this double extraction method.


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OfflineRuaridh McD
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Re: Enokitake Liquid Culture [Re: Forrester]
    #26941851 - 09/18/20 07:12 AM (3 years, 4 months ago)

I have not tried using agar yet because I don`t have a flow hood and it seemed like there was a higher chance of contamination than just using a syringe to get a sample and injecting it through a self healing port. I have managed to get results with this technique and the latest batch of grain spawn looks promising.

but if you are saying go down the agar route I will do more research for sure.


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OfflineForresterM
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Re: Enokitake Liquid Culture [Re: Ruaridh McD]
    #26942920 - 09/18/20 06:06 PM (3 years, 4 months ago)

Yeah the problem with doing it that way is no matter what you're going to get a lot of bacteria along with it, which thrives in liquid culture.  It might work sometimes, with luck, but it's asking for problems.


--------------------
Repugnant is a creature who would squander the ability to lift an eye to heaven, conscious of his fleeting time here.
-------------------

Have some medicinal mushrooms and want to get the most out of them?  Try this double extraction method.


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