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OfflineChumbleton
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BRF inoculation Question
    #26938902 - 09/16/20 11:56 AM (3 years, 4 months ago)

This is my first grow, it's been a lot of fun so far. But I have a quick question about how I inoculated my BRF cakes that I haven't been able to find the answer to using search tek. So, please forgive my noobish-ness.

When I inoculated my 6 1/2 pint jars, I neglected the step that said you need to make sure the needle is against the glass and I inoculated into the substrate itself. (Honestly, I thought it was done for effect to show the inoculate going into the cakes, oops haha) Mind you, I didn't just plunge it right in the middle. I had 4 holes made in my mason jar lids towards the side as I've seen in many PF Tek write ups and videos. So it was still close to the sides, but from what I understand the purpose of inoculating on the side of the glass is to let it drip to the bottom to cover more area. 

It's only day 7 since inoculation and one of my jars is definitely doing well, but the other 5 aren't showing many signs of growth. From everything I've read on this forum, that's totally normal. I just need to ease my mind that I didn't botch a few of them. So with all of that, here are my questions:

1. Did injecting the inoculate into the substrate itself cause the mycelium to fail, or is it just colonizing from the inside-out.

2. Will this inoculation hiccup cause the colonization to fail?

Thanks for taking the time to read these. I hope I've been clear enough.


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OfflineSocrateshroom
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Re: BRF inoculation Question [Re: Chumbleton]
    #26938921 - 09/16/20 12:14 PM (3 years, 4 months ago)

Quote:

Chumbleton said:
1. Did injecting the inoculate into the substrate itself cause the mycelium to fail, or is it just colonizing from the inside-out.

2. Will this inoculation hiccup cause the colonization to fail?

Thanks for taking the time to read these. I hope I've been clear enough.




1) It is most likely colonizing from whatever point you injected (so could be inside out). For experiments sake I once inoculated straight down the middle and, although it seemed like forever before it fully colonized, the time frame was the same it just colonized inside first.

Injecting the solution down near the glass is also a matter of being able to see it as soon as it colonizes (so that you can monitor it). Makes it easier than if you do it down the middle and can't track growth.

2) Either way you are trying to colonize the substrate so you want to inoculate into the substrate itself. What you don't want to do is squeeze half or the entire syringe into your cake as 1) that's just a waste of spore solution as a drop or two is enough & 2) that much solution would probably throw the water content off quite a bit (thus leading to a potential stall).

Be patient. I can see it by day 3 if I inoculate against the glass but it's taken as long as 2 weeks to see something (when I shot straight down the middle).


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OfflineChumbleton
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Re: BRF inoculation Question [Re: Socrateshroom]
    #26938936 - 09/16/20 12:31 PM (3 years, 4 months ago)

Quote:

Socrateshroom said:

2) Either way you are trying to colonize the substrate so you want to inoculate into the substrate itself. What you don't want to do is squeeze half or the entire syringe into your cake as 1) that's just a waste of spore solution as a drop or two is enough & 2) that much solution would probably throw the water content off quite a bit (thus leading to a potential stall).





Thank you so much for the reply!

Regarding 2) That's what I've been reading. Just for anyone else who comes by, I stuck to about .25cc per hole as I've seen described in other write ups and videos. I think a few of the jars have more than 1cc in them however (I've never used a syringe before, it'll get away from you haha), hopefully that won't throw the balance off too much, and if it does, there's always next time! It's all a learning experience.

Thank you again for responding, I see how the main virtue of this hobby is patience.


Edited by Chumbleton (09/16/20 12:33 PM)


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OfflineChumbleton
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Re: BRF inoculation Question [Re: Chumbleton]
    #26939012 - 09/16/20 01:06 PM (3 years, 4 months ago)

To add on another quick question:

3) Will the described situation result in a different consolidation time since it is colonizing from the point of inoculation, which is below the surface? I know in write ups and videos I've watched they say to let the cakes consolidate for a week after the surface is colonized.


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OfflineSocrateshroom
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Registered: 09/05/18
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Re: BRF inoculation Question [Re: Chumbleton]
    #26939031 - 09/16/20 01:15 PM (3 years, 4 months ago)

Quote:

Chumbleton said:
Quote:

Socrateshroom said:

2) Either way you are trying to colonize the substrate so you want to inoculate into the substrate itself. What you don't want to do is squeeze half or the entire syringe into your cake as 1) that's just a waste of spore solution as a drop or two is enough & 2) that much solution would probably throw the water content off quite a bit (thus leading to a potential stall).





Thank you so much for the reply!

Regarding 2) That's what I've been reading. Just for anyone else who comes by, I stuck to about .25cc per hole as I've seen described in other write ups and videos. I think a few of the jars have more than 1cc in them however (I've never used a syringe before, it'll get away from you haha), hopefully that won't throw the balance off too much, and if it does, there's always next time! It's all a learning experience.

Thank you again for responding, I see how the main virtue of this hobby is patience.




I've used .5cc per hole (with 2 holes in the jar) and so, 1cc per jar. And I've never had a problem. But I would recommend using less because you don't need to use so much solution per jar. (And like you said, sometimes it gets away from you and you inoculate with too much by accident. It usually isn't an issue).

Quote:

Chumbleton said:
To add on another quick question:

3) Will the described situation result in a different consolidation time since it is colonizing from the point of inoculation, which is below the surface? I know in write ups and videos I've watched they say to let the cakes consolidate for a week after the surface is colonized.




Everything is up to genetics but, in my experience, it always ends up being about the same. I'd consolidate for 1-2 weeks. There are some arguments about longer consolidation leading to more potent fruits. From what I read, it's a theoretical suggestion, but it at least seems that the extra consolidation doesn't hurt.

Once you see the surface fully colonized, let it consolidate for 1-2 weeks. You may run into a situation where it is ready to go 2 days into consolidation and it starts pinning in the jar. Then you can take it out and fruit it or shred to bulk.


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