Home | Community | Message Board


Psilocybin Summit - Sept 17-20
Please support our sponsors.

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: PhytoExtractum Kratom Powder for Sale, Maeng Da Thai Kratom Leaf Powder   Bridgetown Botanicals CBD Edibles   North Spore Bulk Substrate, Injection Grain Bag, North Spore Mushroom Grow Kits & Cultivation Supplies   Original Sensible Seeds Bulk Cannabis Seeds   Kraken Kratom Red Vein Kratom   Amazon Agar, Coir, Petri Dish, Ultrasonic Humidifier, Vermiculite

Jump to first unread post. Pages: < First | < Back | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 17 | 18 | 19 | 20 | 21 | 22 | 23 | 24 | 25 | 26 | 27 | 28 | 29 | Next >
OnlineDoctor Mario
Still Learning
Male


Registered: 08/07/20
Posts: 184
Loc: USA Flag
Last seen: 2 seconds
Re: Pastywhyte’s Ez dialed monos (moved) [Re: ohfunkya]
    #26937959 - 09/15/20 10:24 PM (3 days, 3 hours ago)

Quote:

LotKid said:
Thats part of figuring out what works best for you. :thumbup:




If I can ever advance from agar to grain I'll be able to figure that out. Had a few minor set backs with agar contams and transfers not doing anything at all assumable because the food coloring I used has red#3 in it.

Quote:

ohfunkya said:
I tried the unmodded box I wasn’t the biggest fan. So far this seems to be a lot more reliable if you’re trying to push out big flushes




What didn't you like about it?


Post Extras: Print Post  Remind Me! Notify Moderator
InvisiblePastywhyteM
Say hello to my little friend
Male User Gallery


Registered: 09/16/12
Posts: 35,393
Loc: Canada Flag
Trusted Cultivator
Re: Pastywhyte’s Ez dialed monos (moved) [Re: Doctor Mario]
    #26937966 - 09/15/20 10:28 PM (3 days, 3 hours ago)

I seriously doubt the Red #3 is the issue.


Post Extras: Print Post  Remind Me! Notify Moderator
OnlineDoctor Mario
Still Learning
Male


Registered: 08/07/20
Posts: 184
Loc: USA Flag
Last seen: 2 seconds
Re: Pastywhyte’s Ez dialed monos (moved) [Re: Pastywhyte]
    #26938095 - 09/15/20 11:48 PM (3 days, 1 hour ago)

Quote:

Pastywhyte said:
I seriously doubt the Red #3 is the issue.




I used the same line of food coloring that D3 is using for his transfers (Wilton Neon Food Coloring). I poured and swabbed 10 magenta colored plates and did a bunch of transfers to the same colored plates. The night before that, I swabbed 8 teal colored plates. The teal colored plates germinated in 3 days, the magenta plates still havent germinated and they were swabbed 14 days ago. The transfers that I took to the magenta plates started getting really weird. The mycelium started growing down inside of the agar and are expanding outward extremely slow. All the transfer plates did this. I finally asked D3monic if he has used the magenta color and he said he stopped using it because the mycelium was acting weird on it and he wasn't sure why.

I've also used the orange color and had great results. Looking at the the ingredients the only difference is the coloring. Everything else is the same. So I did a quick search on what red#3 really is and its Erythrosine. I found this link on erythrosine. Im not smart enough to interpret most or maybe any of it but I think erythrosine has anti fungal/bacterial properties?

I have no evidence to back any of this up but I cant even get mold or bacteria to grow on the pink plates.



Quote:

D3monic said:
Some of my magenta plates would do weird stuff like this



But others looked perfectly fine.




Looks like the myc on his plate is also trying to grown into the agar too.


Edited by Doctor Mario (09/16/20 12:25 AM)


Post Extras: Print Post  Remind Me! Notify Moderator
InvisibleLotKid
Never.Trust.A.Prankster
 User Gallery

Registered: 01/07/17
Posts: 5,294
Loc: Shakedown St.
Trusted Cultivator
Re: Pastywhyte’s Ez dialed monos (moved) [Re: Doctor Mario]
    #26938166 - 09/16/20 12:55 AM (3 days, 47 minutes ago)

And now, for a moment of science...



--------------------

:mushroom2: LotKid's PE Tips        :shpongle:    LotKid's Chocolates :mushroom2:
Grain Strainer


 


Post Extras: Print Post  Remind Me! Notify Moderator
Offlinemeowjinx
Stranger
Registered: 05/20/19
Posts: 376
Last seen: 2 hours, 43 minutes
Re: Pastywhyte’s Ez dialed monos (moved) [Re: Doctor Mario]
    #26938250 - 09/16/20 02:21 AM (2 days, 23 hours ago)

Quote:

Doctor Mario said:
Quote:

Pastywhyte said:
I seriously doubt the Red #3 is the issue.




I used the same line of food coloring that D3 is using for his transfers (Wilton Neon Food Coloring). I poured and swabbed 10 magenta colored plates and did a bunch of transfers to the same colored plates. The night before that, I swabbed 8 teal colored plates. The teal colored plates germinated in 3 days, the magenta plates still havent germinated and they were swabbed 14 days ago. The transfers that I took to the magenta plates started getting really weird. The mycelium started growing down inside of the agar and are expanding outward extremely slow. All the transfer plates did this. I finally asked D3monic if he has used the magenta color and he said he stopped using it because the mycelium was acting weird on it and he wasn't sure why.

I've also used the orange color and had great results. Looking at the the ingredients the only difference is the coloring. Everything else is the same. So I did a quick search on what red#3 really is and its Erythrosine. I found this link on erythrosine. Im not smart enough to interpret most or maybe any of it but I think erythrosine has anti fungal/bacterial properties?

I have no evidence to back any of this up but I cant even get mold or bacteria to grow on the pink plates.



Quote:

D3monic said:
Some of my magenta plates would do weird stuff like this



But others looked perfectly fine.




Looks like the myc on his plate is also trying to grown into the agar too.




Yikes. I just poured some no-pour pasty plates using D3's agar recipe and that magenta food coloring

Good thing I just used those plates as practice for my regular petris, I used that same brand of food coloring, but for the 20 plates I poured I used the green one

Hopefully I don't have issues w/ strange growth, but if I do I'll post it here to add to the data on this brand of magenta food coloring. I'll be transferring to this plate:



Edited by meowjinx (09/16/20 02:24 AM)


Post Extras: Print Post  Remind Me! Notify Moderator
Offlinelovepotion
Unconditional
 User Gallery

Registered: 06/04/06
Posts: 442
Loc: In transit
Last seen: 6 hours, 9 minutes
Re: Pastywhyte’s Ez dialed monos (moved) [Re: LotKid]
    #26938263 - 09/16/20 02:47 AM (2 days, 22 hours ago)

Great, now I’m gonna look for this bug juice on everything that’s red in the grocery store


--------------------
Staying Out to Lunch


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineNightWqtchman
Interdimensional.
 User Gallery


Registered: 08/15/20
Posts: 31
Loc: Omnipresent.
Last seen: 42 minutes, 36 seconds
Re: Pastywhyte’s Ez dialed monos (moved) [Re: meowjinx]
    #26938266 - 09/16/20 02:54 AM (2 days, 22 hours ago)

Pasty, question.

Say I was only doing one tub (I'm only doing one tub).
Adding/removing tape for one tub isn't a big issue for me.

Is there harm in going ahead and taping the holes for colonization?
For purpose of keeping the substrate in optimal condition.
And with the lack of FAE, would that benefit the mycelium, however slightly?
Trying to maximize my chances on my first grow.

Also, I read that your climate is around 15% RH.
Mine is around 40%.

Thanks for the TEK, and everything else you do around here!:flowerchild::heart:


--------------------
There are two kinds of people in this world: Mushroom people, and mold people.
Their mycelium looks almost identical. However, one bears fruit :sporedrop:, the other does not.

(Both kinds of people are contained within you, it's your choice which one you become.)


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineA.k.a
Stranger
 User Gallery

Registered: 10/27/19
Posts: 8,363
Loc: Gaming the system
Last seen: 48 minutes, 56 seconds
Re: Pastywhyte’s Ez dialed monos (moved) [Re: NightWqtchman]
    #26938313 - 09/16/20 04:03 AM (2 days, 21 hours ago)

It won’t hurt but it won’t help either.

Up until recently it was standard to restrict air for 10 days.


--------------------
LAGM2020


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineEdmunterS
Mr
Male User Gallery


Registered: 05/01/13
Posts: 5,585
Last seen: 9 hours, 8 minutes
Re: Pastywhyte’s Ez dialed monos (moved) [Re: A.k.a]
    #26938370 - 09/16/20 06:26 AM (2 days, 19 hours ago)

Second try with these boxes..... Im sold



Unmodified works but the pin sets on these are the winner by far.  The sub just glistens


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineA.k.a
Stranger
 User Gallery

Registered: 10/27/19
Posts: 8,363
Loc: Gaming the system
Last seen: 48 minutes, 56 seconds
Re: Pastywhyte’s Ez dialed monos (moved) [Re: Edmunter]
    #26938403 - 09/16/20 07:19 AM (2 days, 18 hours ago)

Yeah there’s a lot of thick pinsets in here.

I’m finally gonna run one of these for my first mono.

I was thinking I’d need to block the holes up more cuz of my low humidity but I just saw somebody mention Pasty’s got similar levels.


--------------------
LAGM2020


Post Extras: Print Post  Remind Me! Notify Moderator
InvisibleLotKid
Never.Trust.A.Prankster
 User Gallery

Registered: 01/07/17
Posts: 5,294
Loc: Shakedown St.
Trusted Cultivator
Re: Pastywhyte’s Ez dialed monos (moved) [Re: lovepotion]
    #26938437 - 09/16/20 08:01 AM (2 days, 17 hours ago)

Quote:

lovepotion said:
Great, now I’m gonna look for this bug juice on everything that’s red in the grocery store



Before you get to the grocery store, check this out...



--------------------

:mushroom2: LotKid's PE Tips        :shpongle:    LotKid's Chocolates :mushroom2:
Grain Strainer


 


Post Extras: Print Post  Remind Me! Notify Moderator
InvisiblePastywhyteM
Say hello to my little friend
Male User Gallery


Registered: 09/16/12
Posts: 35,393
Loc: Canada Flag
Trusted Cultivator
Re: Pastywhyte’s Ez dialed monos (moved) [Re: LotKid]
    #26938569 - 09/16/20 10:26 AM (2 days, 15 hours ago)

I had a quick look on erythrosine and I see nothing to suggest antifungal properties. I’m not saying the food colouring isn’t the issue however, I’m not convinced that the erythrosine is the reason.

On humidity, it’s pretty dry where I live. If I drill the optional holes I need to mist occasionally to keep surface conditions up. If you live in similar RH and don’t want to have to mist, don’t drill or tape up the optional hole as needed.


Post Extras: Print Post  Remind Me! Notify Moderator
OnlineDoctor Mario
Still Learning
Male


Registered: 08/07/20
Posts: 184
Loc: USA Flag
Last seen: 2 seconds
Re: Pastywhyte’s Ez dialed monos (moved) [Re: Pastywhyte]
    #26938770 - 09/16/20 12:31 PM (2 days, 13 hours ago)

Quote:

Pastywhyte said:
I had a quick look on erythrosine and I see nothing to suggest antifungal properties. I’m not saying the food colouring isn’t the issue however, I’m not convinced that the erythrosine is the reason.

On humidity, it’s pretty dry where I live. If I drill the optional holes I need to mist occasionally to keep surface conditions up. If you live in similar RH and don’t want to have to mist, don’t drill or tape up the optional hole as needed.




What tests can we run to find out whats causing it? It would probably be a good idea to make another batch of agar with the same food coloring and take some more transfers to it. If it does weird shit again then it would be safe to assume its definitely something in that food coloring but then what? What other tests can we run?

Everything that is in that food coloring is in the teal color except for the erythrosine and the teal works great.


Post Extras: Print Post  Remind Me! Notify Moderator
InvisiblePastywhyteM
Say hello to my little friend
Male User Gallery


Registered: 09/16/12
Posts: 35,393
Loc: Canada Flag
Trusted Cultivator
Re: Pastywhyte’s Ez dialed monos (moved) [Re: Doctor Mario]
    #26938803 - 09/16/20 12:50 PM (2 days, 12 hours ago)

I would start over with a set of clones or isolates. Then test different nutes and concentrations, different agars, etc but all with the same food colouring. Then observe. It’s possible you both just lost the genetics dice roll. Maybe the genetics you found just prefer different nutes. If they all suffer the same fate then I would think you were maybe on to something with the food color.


Post Extras: Print Post  Remind Me! Notify Moderator
InvisibleMuthaFudpuckerS
Just Strange

Registered: 03/29/19
Posts: 175
Re: Pastywhyte’s Ez dialed monos (moved) [Re: Pastywhyte]
    #26938808 - 09/16/20 12:55 PM (2 days, 12 hours ago)

I am in the desert and it has been wicked dry this year. I did PE with a coir pseudo casing and I think the optional holes would have helped. I am testing that in a side by side on the next run. I feel like my fae was too low for the casing. I may just have to mist more. I do run a wicking humidifier in the grow room, but I don't think it has much overall effect. I will be adding verm this time as well. Hope to have a comparison to show soon. A2G 3 days ago so still have some time to wait.


--------------------
My first grow journal - Know nothing noob's notes
Blue juice cold extraction trial
How to succeed in mycology (and life) - know nothing, read everything, try something, and accept advice.


Post Extras: Print Post  Remind Me! Notify Moderator
OnlineDoctor Mario
Still Learning
Male


Registered: 08/07/20
Posts: 184
Loc: USA Flag
Last seen: 2 seconds
Re: Pastywhyte’s Ez dialed monos (moved) [Re: Pastywhyte]
    #26938810 - 09/16/20 12:56 PM (2 days, 12 hours ago)

I kind of want to try this but at the same time for what? Just to say, "Don't use this color in your agar plates"?


Post Extras: Print Post  Remind Me! Notify Moderator
InvisiblePastywhyteM
Say hello to my little friend
Male User Gallery


Registered: 09/16/12
Posts: 35,393
Loc: Canada Flag
Trusted Cultivator
Re: Pastywhyte’s Ez dialed monos (moved) [Re: Doctor Mario]
    #26938863 - 09/16/20 01:37 PM (2 days, 12 hours ago)

Do it because you’re curious. Or don’t, i was just outlining how I would do it if I wanted results that had control.


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineNightWqtchman
Interdimensional.
 User Gallery


Registered: 08/15/20
Posts: 31
Loc: Omnipresent.
Last seen: 42 minutes, 36 seconds
Re: Pastywhyte’s Ez dialed monos (moved) [Re: Pastywhyte]
    #26938983 - 09/16/20 02:54 PM (2 days, 10 hours ago)

Quote:

LotKid said:
Quote:

lovepotion said:
Great, now I’m gonna look for this bug juice on everything that’s red in the grocery store



Before you get to the grocery store, check this out...






Bruh... :puke:
Thanks for sharing the info.
I know what I'll never be touching again.

Quote:

Pastywhyte said:
On humidity, it’s pretty dry where I live. If I drill the optional holes I need to mist occasionally to keep surface conditions up. If you live in similar RH and don’t want to have to mist, don’t drill or tape up the optional hole as needed.




Thanks bro, much appreciated. :takingnotes:  :bow2:


--------------------
There are two kinds of people in this world: Mushroom people, and mold people.
Their mycelium looks almost identical. However, one bears fruit :sporedrop:, the other does not.

(Both kinds of people are contained within you, it's your choice which one you become.)


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineA.k.a
Stranger
 User Gallery

Registered: 10/27/19
Posts: 8,363
Loc: Gaming the system
Last seen: 48 minutes, 56 seconds
Re: Pastywhyte’s Ez dialed monos (moved) [Re: NightWqtchman]
    #26939044 - 09/16/20 03:22 PM (2 days, 10 hours ago)

I’ve also noticed slight differences in myc based on food color, but mine was with neon blue.


I’ve got an average of like 12% rh most of the time, so just skip the extra one and it should be good??


--------------------
LAGM2020


Post Extras: Print Post  Remind Me! Notify Moderator
InvisiblePastywhyteM
Say hello to my little friend
Male User Gallery


Registered: 09/16/12
Posts: 35,393
Loc: Canada Flag
Trusted Cultivator
Re: Pastywhyte’s Ez dialed monos (moved) [Re: A.k.a]
    #26939147 - 09/16/20 04:07 PM (2 days, 9 hours ago)

Yeah if you want set and forget in a dry climate I would skip the optional hole at first.


Post Extras: Print Post  Remind Me! Notify Moderator
Jump to top. Pages: < First | < Back | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 17 | 18 | 19 | 20 | 21 | 22 | 23 | 24 | 25 | 26 | 27 | 28 | 29 | Next >

Shop: PhytoExtractum Kratom Powder for Sale, Maeng Da Thai Kratom Leaf Powder   Bridgetown Botanicals CBD Edibles   North Spore Bulk Substrate, Injection Grain Bag, North Spore Mushroom Grow Kits & Cultivation Supplies   Original Sensible Seeds Bulk Cannabis Seeds   Kraken Kratom Red Vein Kratom   Amazon Agar, Coir, Petri Dish, Ultrasonic Humidifier, Vermiculite

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

Similar ThreadsPosterViewsRepliesLast post
* Pastywhyte's Easy Lid Tek
( 1 2 3 4 ... 13 14 )
PastywhyteM 39,870 269 09/18/20 02:47 PM
by madyogi
* How much FAE does agar need? parafilm vs micropore vs ketchup cup redpillcottage 90 4 09/18/20 09:13 AM
by Roger Clemency
* Mono tubs/Double tubs with tyvex? jkklim 970 3 04/04/06 09:07 AM
by Cubenisseur
* monotub - polyfill or micropore tape holes? tokey666 10,042 8 02/28/06 05:25 PM
by tiny_rabid_birds
* Mono-Tub vs Double Tub
( 1 2 all )
unknown28 10,888 31 05/05/18 08:15 AM
by Freedom1
* First time grower using EZ Kit, have quick Q re: harvesting, w/ pics vitabrevis 785 4 05/29/07 01:15 AM
by vitabrevis
* mono tub rye poo TheStudent 2,983 14 05/05/18 08:17 AM
by bam
* Growing at the EZ storage the spiral 913 8 04/15/03 11:22 PM
by JssMthrFcknChrst

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, FooMan, mushboy, fahtster, LogicaL Chaos, 13shrooms, stonesun, wildernessjunkie, cronicr, Pastywhyte, bodhisatta
20,147 topic views. 17 members, 103 guests and 26 web crawlers are browsing this forum.
[ Print Topic ]
Search this thread:
Cannabis Seeds - Original Sensible Seeds
Please support our sponsors.

Copyright 1997-2020 Mind Media. Some rights reserved.

Generated in 0.062 seconds spending 0.006 seconds on 15 queries.