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Offlinekoma23
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Registered: 09/15/20
Posts: 1
Last seen: 1 day, 15 hours
Re: Recognizing and dealing with contamination [Re: Tolldom]
    #26936352 - 09/15/20 06:30 AM (3 days, 19 hours ago)

Hi all! So I ordered a premade box. I was lazy at growing and ran out of shrooms and needed some asap. I figured that before I created my jars, these would already be ready for the first harvest.

After like 24h, the box started showing this. Is this cobweb, did I got shipped a contaminated box, is the box a goner? Tnx < 3



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OfflineFarmerEric
Friend
Registered: 04/06/20
Posts: 24
Last seen: 2 days, 11 hours
Re: Recognizing and dealing with contamination [Re: koma23]
    #26936978 - 09/15/20 02:33 PM (3 days, 11 hours ago)



Total noob still learning. Is this mold or myc? Is there a guide so I can learn to ID the difference?


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OfflineAlsetAlokinS
Student
Registered: 07/30/20
Posts: 100
Last seen: 3 hours, 30 minutes
Re: Recognizing and dealing with contamination [Re: FarmerEric]
    #26937122 - 09/15/20 03:48 PM (3 days, 10 hours ago)

Quote:

FarmerEric said:


Total noob still learning. Is this mold or myc? Is there a guide so I can learn to ID the difference?




Not a veteran, but looks like a lot of myc genetics which is common from multispore.

At this point it is difficult to even see contamination if it is in there. Things like bacteria like to hide.

Transfer a couple of the leading edges to new plates and observe the growth from there. You could think of it as zoomimg in. Right now you're at 10x magnification, next plate will be 100x etc...

Tldr: looks good to me. Wouldn't put it to grain yet, but you're on the right track.


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OfflineFarmerEric
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Registered: 04/06/20
Posts: 24
Last seen: 2 days, 11 hours
Re: Recognizing and dealing with contamination [Re: AlsetAlokin]
    #26937849 - 09/15/20 09:01 PM (3 days, 5 hours ago)

Quote:

AlsetAlokin said:
Quote:

FarmerEric said:


Total noob still learning. Is this mold or myc? Is there a guide so I can learn to ID the difference?




Not a veteran, but looks like a lot of myc genetics which is common from multispore.

At this point it is difficult to even see contamination if it is in there. Things like bacteria like to hide.

Transfer a couple of the leading edges to new plates and observe the growth from there. You could think of it as zoomimg in. Right now you're at 10x magnification, next plate will be 100x etc...

Tldr: looks good to me. Wouldn't put it to grain yet, but you're on the right track.





Thank you, what do you think of this wild isolation?



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Offlineeucario
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Registered: 07/25/20
Posts: 6
Last seen: 2 days, 8 hours
Re: Recognizing and dealing with contamination [Re: FarmerEric]
    #26938658 - 09/16/20 11:29 AM (2 days, 14 hours ago)

Hi guys, started my 2nd culture and this time I didn't have syringes ready to inoculate so I made my own from spore prints. After 1 week I started seeing these black spots in almost every jar, is it black mold? Am I fucked and will have to throw everything away? In case it is black mold, what could have caused the contamination? I didn't flame the syringe after every inoculation (yes, absolutely stupid) so I'm afraid it could have been that (used glove box, btw).







Edited by eucario (09/16/20 11:37 AM)


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OfflineFarmerEric
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Registered: 04/06/20
Posts: 24
Last seen: 2 days, 11 hours
Re: Recognizing and dealing with contamination [Re: eucario]
    #26938995 - 09/16/20 02:58 PM (2 days, 11 hours ago)

Quote:

eucario said:
Hi guys, started my 2nd culture and this time I didn't have syringes ready to inoculate so I made my own from spore prints. After 1 week I started seeing these black spots in almost every jar, is it black mold? Am I fucked and will have to throw everything away? In case it is black mold, what could have caused the contamination? I didn't flame the syringe after every inoculation (yes, absolutely stupid) so I'm afraid it could have been that (used glove box, btw).










Did you consider your spore print was contaminated?


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InvisibleWyoMX
Stranger
Male
Registered: 07/06/15
Posts: 864
Loc: PNW
Re: Recognizing and dealing with contamination [Re: eucario]
    #26939062 - 09/16/20 03:29 PM (2 days, 10 hours ago)

My first guess would be dirty syringe first then after that the glovebox. If those were my jars i would throw them out.


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Offlineeucario
Stranger
Registered: 07/25/20
Posts: 6
Last seen: 2 days, 8 hours
Re: Recognizing and dealing with contamination [Re: WyoMX]
    #26939271 - 09/16/20 05:16 PM (2 days, 8 hours ago)

Quote:

WyoMX said:
My first guess would be dirty syringe first then after that the glovebox. If those were my jars i would throw them out.




Yeah. Probably because I forgot to flame it after each injection, or maybe the air inside the glove box was contaminated as well.

If those spots don't grow would you throw them as well?


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Offlineeucario
Stranger
Registered: 07/25/20
Posts: 6
Last seen: 2 days, 8 hours
Re: Recognizing and dealing with contamination [Re: FarmerEric]
    #26939276 - 09/16/20 05:17 PM (2 days, 8 hours ago)

Quote:

FarmerEric said:
Quote:

eucario said:
Hi guys, started my 2nd culture and this time I didn't have syringes ready to inoculate so I made my own from spore prints. After 1 week I started seeing these black spots in almost every jar, is it black mold? Am I fucked and will have to throw everything away? In case it is black mold, what could have caused the contamination? I didn't flame the syringe after every inoculation (yes, absolutely stupid) so I'm afraid it could have been that (used glove box, btw).










Did you consider your spore print was contaminated?




Maybe. It would be really bad bc I have some spores left I was planning to use in case I throw these out. What is the best way to store them to avoid contam?


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OfflineNibs
Peasant
 User Gallery

Registered: 02/17/20
Posts: 22
Loc: 🇨🇦
Last seen: 1 day, 20 hours
Re: Recognizing and dealing with contamination [Re: eucario]
    #26940063 - 09/17/20 05:28 AM (1 day, 20 hours ago)

Hey homie`s, I`m having some issues identifying if this is bacteria I`m dealing with. Every one of my culture plates I got from an APE swab look like this. there is clear sectoring so im thinking its just very different mycelium than what I`m used to.


When it goes to jars though it ends up looking like this, which really convinced me its bacteria because of the uncolonized grains pressed against the glass, how thick it looks, and some of the jars i left for a while continued to fuzz up nearly to the lid and almost turn into solid bricks.



I ended up putting some in a mini mono and everything seems to be looking fine with the pins coming in, so now i have no idea if this is clean myc or not...


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InvisibleShroomluvr69
luvr of shrooms


Registered: 09/25/19
Posts: 30
Loc: The Netherlands Flag
Re: Recognizing and dealing with contamination [Re: Nibs]
    #26940100 - 09/17/20 06:48 AM (1 day, 19 hours ago)

I have some odd fungus growing I think. At least, it doesn't look like the P. cubensis I know... It has weird black dots in the mycellenium/hairs.


--------------------
Interested in Psilocybe azurescens, Panaeolus cyanescens and Psilocybe mexicana spores!


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OfflineWoolyMarmot
Stranger
Registered: 06/01/20
Posts: 28
Last seen: 2 hours, 57 minutes
Re: Recognizing and dealing with contamination [Re: Shroomluvr69]
    #26940145 - 09/17/20 07:56 AM (1 day, 18 hours ago)

cobweb


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Offlinexgfnxf
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 User Gallery
Registered: 04/28/20
Posts: 6
Last seen: 1 hour, 50 minutes
Re: Recognizing and dealing with contamination [Re: WoolyMarmot]
    #26943241 - 09/19/20 12:12 AM (1 hour, 50 minutes ago)

I accidentally unloaded some contaminated spawn into my monotub substrate. it smelt and looked green.  I tried to remove it but it spread throughout the substrate.  Stemmed to slow down was pinning started.  Kind like thin licen. Thoughts?
https://files.shroomery.org/files/20-38/048862518-IMG_20200916_165643_916.jpg


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Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

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