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Offline6Curi0us.M0nkey9
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Noob: Is this contamination or metabolites?
    #26935437 - 09/14/20 03:41 PM (3 years, 4 months ago)

I have eight PF jars colonizing at the moment and I am actually fairly proud of the results. They are creeping up on being fully colonized and have primarily rhizomorphic growth (inoculated with MS). Though, while checking on them today, I noticed some abnormalities with only one of my jars. It is yellowish/brownish and the growth has stalled in the area of interest. Would anyone please help me identify the issue?

First pic is of the jar in question, the second jar is one of the healthy ones (for reference).

Thank you in advance! May your day be filled with sparkly magic and sunshine.



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“And those who were seen dancing were thought to be insane by those who could not hear the music”
                -Friedrich Nietzsche


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Offlinetryptkaloids
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Re: Noob: Is this contamination or metabolites? [Re: 6Curi0us.M0nkey9]
    #26935462 - 09/14/20 03:52 PM (3 years, 4 months ago)

Contam. Second pic looks fine


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
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Offline6Curi0us.M0nkey9
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Re: Noob: Is this contamination or metabolites? [Re: tryptkaloids]
    #26935466 - 09/14/20 03:53 PM (3 years, 4 months ago)

Interesting, you can tell because of the color, the stalled growth, or the location?


--------------------
“And those who were seen dancing were thought to be insane by those who could not hear the music”
                -Friedrich Nietzsche


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Offlinetryptkaloids
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Re: Noob: Is this contamination or metabolites? [Re: 6Curi0us.M0nkey9]
    #26935473 - 09/14/20 03:56 PM (3 years, 4 months ago)

Color and texture. Could also be burnt. Did that spot get direct sunlight or a refraction off another jar heat that spot?


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 


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Offline6Curi0us.M0nkey9
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Registered: 05/03/20
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Re: Noob: Is this contamination or metabolites? [Re: tryptkaloids]
    #26935715 - 09/14/20 06:21 PM (3 years, 4 months ago)

Definitely not. I keep them in a dark, cool closet. I’m just confused as to how only one of them were contaminated, and why it would’ve gotten contaminated at the bottom. If it were closer to the verm layer it would make sense that contams got in during incubation. Though due to the location it almost makes me question the effectiveness of the sterilization process, but I have 18 jars total (10 WBS) and it’s the only one looking like this.


--------------------
“And those who were seen dancing were thought to be insane by those who could not hear the music”
                -Friedrich Nietzsche


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Offline6Curi0us.M0nkey9
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Re: Noob: Is this contamination or metabolites? [Re: 6Curi0us.M0nkey9]
    #26935716 - 09/14/20 06:21 PM (3 years, 4 months ago)

Thank you for your help btw🙏


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“And those who were seen dancing were thought to be insane by those who could not hear the music”
                -Friedrich Nietzsche


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Invisiblehobbyguy
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Re: Noob: Is this contamination or metabolites? [Re: 6Curi0us.M0nkey9]
    #26935749 - 09/14/20 06:47 PM (3 years, 4 months ago)

Could be anything. Contam in syringe that shot out in that one jar, jar didn't sterilize correctly, ect. It happens.

BTW no need to keep in a cool dark closet. I put jars on my dresser


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Offline6Curi0us.M0nkey9
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I'm a teapot

Registered: 05/03/20
Posts: 51
Loc: Nirvana
Last seen: 2 years, 6 months
Re: Noob: Is this contamination or metabolites? [Re: hobbyguy]
    #26937465 - 09/15/20 04:14 PM (3 years, 4 months ago)

Yeah I know. The location is arbitrary other than the fact it it is the most convenient place to store them and is a suitable place to do so. Thank you for your help, I dumped the jar in my compost and there was trichoderma😬. Thanks for the advice.


--------------------
“And those who were seen dancing were thought to be insane by those who could not hear the music”
                -Friedrich Nietzsche


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