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OfflinePsychedelicjunkie
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Registered: 07/05/20
Posts: 66
Last seen: 1 year, 10 months
3 jars not colonizing
    #26937310 - 09/15/20 03:03 PM (3 years, 4 months ago)

Hey guys! I nocced up 20 jars and I have 3 that aren't colonizing at all, and I have 1 that is already fully colonized, and 4 others who are almost done, it seems weird. I let them dry for about 2 hours, in 6 strainers so they would dry faster. Did I make them too dry or what could it have been?


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Offlinepolyflakes
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Registered: 03/27/08
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Last seen: 6 months, 22 days
Re: 3 jars not colonizing [Re: Psychedelicjunkie]
    #26937327 - 09/15/20 03:10 PM (3 years, 4 months ago)

What did you use to knock them up with? Maybe just didnt get enough spores or genetic material in it? 

Either way you didn't give enough info to do anything more then guess.


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OfflinePsychedelicjunkie
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Registered: 07/05/20
Posts: 66
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Re: 3 jars not colonizing [Re: polyflakes]
    #26937339 - 09/15/20 03:15 PM (3 years, 4 months ago)

Arh yeah okay sorry. I nocced them up with a MS syringe. The first 10 was 23/8 the next 10 was 28/8. Rye grains, poly lids


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Offlinepolyflakes
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Registered: 03/27/08
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Re: 3 jars not colonizing [Re: Psychedelicjunkie]
    #26937373 - 09/15/20 03:28 PM (3 years, 4 months ago)

Yeah just may not have gotten enough spores with the right genetics together to enable growth. Look in to agar work its easier then you may think and your results will be much better in most aspects of your runs.


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OfflinePsychedelicjunkie
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Registered: 07/05/20
Posts: 66
Last seen: 1 year, 10 months
Re: 3 jars not colonizing [Re: polyflakes]
    #26937412 - 09/15/20 03:51 PM (3 years, 4 months ago)

Yeah im already doing it, I just started growing for the first time, and figured I'd use a spore syringe to begin with, and make agar aswell.  I did 1 transfer, and my t1 plates are growing now. Imma do a few more transfers till I'm certain I have a clean plate.


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