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Mycobro420
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Registered: 06/01/20
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Shelf life of dormant agar plates
#26937298 - 09/15/20 02:59 PM (3 years, 4 months ago) |
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My method of storage is in a fridge with fully colonized dishes with their own individual sandwich bag inside a insolated lunch box to avoid temp swings when fridge is opened I think oldest plate mabey 4-5 months no condensation or pooling water in the plates are they still safe for transfers
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polyflakes
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Re: Shelf life of dormant agar plates [Re: Mycobro420]
#26937336 - 09/15/20 03:14 PM (3 years, 4 months ago) |
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look up slants if your goal is long turn storage of cultures. Its the best and longest lasting method for storing.
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Mycobro420
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Re: Shelf life of dormant agar plates [Re: polyflakes]
#26937374 - 09/15/20 03:29 PM (3 years, 4 months ago) |
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I understand slants for for long term storage but I wanted to know if the ones I got are still usable
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Land Trout
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Re: Shelf life of dormant agar plates [Re: Mycobro420]
#26937384 - 09/15/20 03:33 PM (3 years, 4 months ago) |
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I’ve stored plates in the fridge for well over six months with way less control than what you’ve described and it’s always worked well for me. I think the longest I’ve had one in the fridge was 8 months, and it worked great when I knocked up grain with it.
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polyflakes
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Re: Shelf life of dormant agar plates [Re: Land Trout]
#26937408 - 09/15/20 03:48 PM (3 years, 4 months ago) |
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You can always just take it out and put a wedge on a fresh plate make sure its still clean and viable should be fine though.
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xspak
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Registered: 06/08/20
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Re: Shelf life of dormant agar plates [Re: polyflakes]
#26937479 - 09/15/20 04:21 PM (3 years, 4 months ago) |
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I had a similar situation lately. Two types Pan Cyan Multispore on two plates. In the fridge since about 16 months. Plenty condensation, pools of water, mycelium looked f§$"d on both of them.
Took them out a week ago and made transfers to new plates.
One of them grows nicely so far. The other one was dead. But I do not yet know if the one still alive will still fruit. But on the agar it looks normal.
I would take them out, let them get used to the temperature. Ideally you will already see that they start to grow again. Transfer to new plates.
I think if you have cubes or stone producers you should be fine. They seem to be able to withstand rough handling. Pans mycelium seems more fragile.
But I'm not a TC, so my experience is limited.
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Ep1429
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Re: Shelf life of dormant agar plates [Re: polyflakes]
#26937483 - 09/15/20 04:22 PM (3 years, 4 months ago) |
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I’ve stored plates in the fridge for two years, and they wouldn’t grow after I tried to transfer from them, even though the mycelium was still white.
You need to take the plates out and make a transfer to a new plate once or twice a year to keep the culture fresh.
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Mycobro420
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Re: Shelf life of dormant agar plates [Re: Ep1429]
#26937576 - 09/15/20 05:06 PM (3 years, 4 months ago) |
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Ok sounds good guys thanks I transfer them to new plates an I'll see what happens
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