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Offlinepolyflakes
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Experiment with old lc. <updated OP>
    #26936135 - 09/14/20 11:19 PM (3 years, 4 months ago)

So I am getting started some pe and gt on agar so while I am wanting on that I had a year or so old lc that I had kept just out on a shelf. It's b+ off like a 3rd transfer away from MS.

Aways I knocked up 9 jars with it. Now stupid me forgot to mix up the LC before putting it in th sab with the 9 jars. Did a very poor slosh and knocked them up.

6 looked good though only growing from a couple small areas but it looked healthy so. The other 3 weren't showing any growth so I reinoculated with the same LC after mixing the fuck out of the LC. They grew fast everywhere the LC hit and looked good then I saw a little odd growth.
It was white but just didn't look right and after the shake the 3 still look off definitely not going to spawn them.

Below are current pics of the jars and the LC. The healthier looking jars may be a touch bacterial but i think they will be good to spawn.




Update:
So took five of the good jars and made four shoeboxes 3 with 1qt spawn and 1 with 2qt. Tried to keep them at about half way up though had to make them a little short due to not having as much spawn as I thought woth the lower spawn ratio. not sure exactly the ratio used.

Thats 4 days since spawning them.




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Edited by polyflakes (09/21/20 09:00 PM)


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OfflineForresterM
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Re: Experiment with old lc. [Re: polyflakes]
    #26936306 - 09/15/20 02:51 AM (3 years, 4 months ago)

I've used LC well over a year old and not had any problems.

Man I'd be worried about opening up that jar you got with all the tyvek around the lid and not knocking a spore or two in the jar though!  I always make my LC lids super easy to get in and out of to avoid issues.  That's just me though.


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Offlinepolyflakes
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Re: Experiment with old lc. [Re: Forrester]
    #26936390 - 09/15/20 05:22 AM (3 years, 4 months ago)

There is an injection port on the lid the lid is never removed.


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OfflineA.k.aM
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Re: Experiment with old lc. [Re: polyflakes]
    #26936459 - 09/15/20 06:54 AM (3 years, 4 months ago)

For sure a lot of mold in that pic of three jars, especially the one on the right.


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Offlinepolyflakes
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Re: Experiment with old lc. [Re: A.k.a]
    #26936465 - 09/15/20 06:58 AM (3 years, 4 months ago)

I am watching it a letting grow a bit so I can see what develops. I haven't had a bunch of failures so anything I out of the norm could help me learn.


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OfflineA.k.aM
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Re: Experiment with old lc. [Re: polyflakes]
    #26936470 - 09/15/20 07:00 AM (3 years, 4 months ago)

I do the same thing.

I’m betting you’ll see a lot of it turn green in the next couple days.


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Offlinepolyflakes
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Re: Experiment with old lc. [Re: A.k.a]
    #26936474 - 09/15/20 07:04 AM (3 years, 4 months ago)

Yeah the growth other then mycelium beofe the shake was just covering the surface of the seeds. Almost looked like it had been poorly spray painted with white felt if that makes any sense.


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Offlinepolyflakes
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Re: Experiment with old lc. [Re: polyflakes]
    #26948273 - 09/21/20 06:18 PM (3 years, 4 months ago)

updated op if anyone's interested


Edited by polyflakes (09/21/20 06:19 PM)


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