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Wrightii
Stranger Danger



Registered: 05/15/20
Posts: 216
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always blame the vendor! (agar issues)
#26936395 - 09/15/20 05:32 AM (3 years, 4 months ago) |
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Hey people!
This is my first post here on the shroomery. Have been a lurker for quite a while now but it's time to get real. Please go easy on me  Also; sorry for (possible) bad English.
So a while back I ordered a vial with P. cyanescens "Jamaican" spores from a reputable vendor with the intention to germinate them on LME agar (as I usually do with prints or syringes). Normally this poses no problems what so ever -that is; non that cannot be transfered away. I work in front of a HEPA flowhood and abundant contaminations are pretty much mostly exclusive (though still not regular) to the process of cloning (wild) fruitbodies or rampage "I don't care anymore" transfering. Contamination as bad as I got from this particular spore vial I might say just never happens:

This pic was taken after about (I guess) 8 days incubation at 25 degrees Celcius (77F). Note that the green agar is due to me adding some foodcoloring. I like my plates a bit festive Ah and one of the plates is me also testing a LC i started with the spores (risky habits i know, but's just a hobby). The LC only seemed to have amplified the rate of contamination.
Since this was so unusual and never happend before I decided to contact the vendor to see if we could figure something out. They were really chill and quick about it, and offered me either a voucher or a new free spore vial of the Paneolus. Great! So i decided to try the spore vial again (I really want to try Paneolus) and got a new one delivered about three days later. Thing is, the exact same thing is happening all over again:


Is there someone with any idea on what could be happening here? I inoculated 6 plates and all of them had (what i guess is) trichoderma growing, while non of them showed any sign of Paneolus mycelium formation (also not the whispey kind). Today I dropped spores on 4 more plates, just to be sure. I feel a bit silly about contacting the vendor again, since I might -as far as they know- just be a huge newbie. Maybe I even am . I've done quite some germinations, transfers, clones and what not by now though, and just don't see what i might be doing wrong.
Furthermore; why are these spores so damn expensive at vendors (20-30 euro for a vial). Once you get going making sporeprints costs next to nothing! Putting the spores in an airtight "sterile" vial just seems unnecesarry. And even if the prints are not the cleanest who cares, just agar that stuff. Next to maybe finding suggestions to what i might be doing wrong (might be hard to say not seeing me work) I'm also posting because i found that there is a trading forum for sporeprints on the shroomery. Noice! Less money more trading! I need 50 posts to join though... (1/50). Does anybody know if there are active participants and trades happening? Then i'll push on to reach those 50 posts! And (i hope this is allowed, if not sorry and disregard) if anybody feels like trading some paneolus spores for different cubensis spores or slants of edible mushrooms feel free to message me. We'll have to check what the shipping possibilities are.
Hope this post was clear and eazy to follow  In any case; thanks for having a look!
-Wii
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A.k.a
Stranger



Registered: 10/27/19
Posts: 16,782
Loc: Gaming the system
Last seen: 2 hours, 25 minutes
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Re: always blame the vendor! (agar issues) [Re: Wrightii]
#26936457 - 09/15/20 06:50 AM (3 years, 4 months ago) |
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That is a gross contam.
What do you mean a vial?? Like an actual glass tube?
I’ve only ever seen syringes or prints on foil.
How are you getting the spores on there? If you can clone and do transfers without contam then it should be fine.
I’d guess it’s the vendor who took a bunch of spores from a contaminated grow since you’re only getting one type of mold over and over.
There’s plenty of people here who will hook up good prints internationally, that’s prob the way I’d go.
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LAGM2020     
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Wrightii
Stranger Danger



Registered: 05/15/20
Posts: 216
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Re: always blame the vendor! (agar issues) [Re: A.k.a]
#26936576 - 09/15/20 08:38 AM (3 years, 4 months ago) |
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I mean these types of things:

They are marketed as the bees knees, but i find them horrible. its impossible to completely empty them due to the vacuum that forms. Sometimes they just suck in air next to the needle, which could possibly be contaminated.
I just use a 0.5ml syringe to drop some small droplets here and there.
Unfortunatly this was as far as i could find the only way to aquire spores for P. cyanescense `jamaica` in my area. I did decide to contact the vendor again, informing him that this one was also contaminated. He refunded everything and even added a small bonus for compensation. Great website. Can we just mention vendors by name btw in posts?
Edited by Wrightii (09/15/20 08:39 AM)
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