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Offlinemeowjinx
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How To Tell If There's Sufficient GE in Unmodified Lids?
    #26936239 - 09/15/20 01:02 AM (3 years, 4 months ago)

I have several quart jars colonizing right now. They were all inoculated on the same day, all individually inoculated w/ small agar wedges of the same PE clone

The grain is horse oats that I prepped in 2 batches. I'm seeing inconsistency in speed of colonization and it's making me wonder if some of these could be stalling due to CO2 build-up. I don't want my jars to stall and die, so I'd like to know how I can tell if there is sufficient GE to ensure colonization?

Pastywhyte is my go-to for TEKs. I use his monotub teks, his no-pour agar tek, I'll be using his LC tek soon. But in his write-up for unmodded lids he says about 0.5 to 0.75 inch turn from "finger tight". I had a hard time assessing how loose they should be, I'm afraid I left them on too tight







Edited by meowjinx (09/15/20 01:03 AM)


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InvisibleEminence
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Re: How To Tell If There's Sufficient GE in Unmodified Lids? [Re: meowjinx]
    #26936332 - 09/15/20 03:55 AM (3 years, 4 months ago)

Just loosen the slow ones another quarter or half inch. I mean, I can't say from experience because I have always had all mine with micropore tape it syringe filters. That's what I'd do at least though. Idk what else you even could do. Why unmodded though? You can get a roll of micropore for like 2 bucks at a lot of drug stores and it'd last a while. Unless you use the jars for other things when you're not using them for this.


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OfflineA.k.aM
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Re: How To Tell If There's Sufficient GE in Unmodified Lids? [Re: Eminence]
    #26936469 - 09/15/20 07:00 AM (3 years, 4 months ago)

I recently got a bunch of these too. If a jar seems to be going slow then loosen it like the smallest amount possible and give it a day, then repeat it necessary.

My first run with them I kept them just a little bit loose and some stalled until I turned the lid a little more then they blew up. However this second run I kept them too loose and they all got mold. But I had the lids very loose and the jars were in a pretty dirty spot.

I’m leaning towards a small hole with tape myself. The main reason I got these was to only have to deal with one piece while knocking them up.


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Offlinemeowjinx
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Re: How To Tell If There's Sufficient GE in Unmodified Lids? [Re: A.k.a]
    #26936527 - 09/15/20 08:06 AM (3 years, 4 months ago)

Quote:

Eminence said:
Just loosen the slow ones another quarter or half inch. I mean, I can't say from experience because I have always had all mine with micropore tape it syringe filters. That's what I'd do at least though. Idk what else you even could do. Why unmodded though? You can get a roll of micropore for like 2 bucks at a lot of drug stores and it'd last a while. Unless you use the jars for other things when you're not using them for this.




Yeah, I'll try loosening a bit, and yeah that's basically all you can do lol. And unmodded because every time I figured I wouldn't have to worry about a filter failing and mold spores from my last grow finding their way into the jars

Quote:

A.k.a said:
I recently got a bunch of these too. If a jar seems to be going slow then loosen it like the smallest amount possible and give it a day, then repeat it necessary.

My first run with them I kept them just a little bit loose and some stalled until I turned the lid a little more then they blew up. However this second run I kept them too loose and they all got mold. But I had the lids very loose and the jars were in a pretty dirty spot.

I’m leaning towards a small hole with tape myself. The main reason I got these was to only have to deal with one piece while knocking them up.




Yes, that's what I've been doing and hopefully it works. Yes, that's exactly what I got them for, too. I definitely prefer plastic lids over metal ones because it makes G2G transfers so much easier. I think I'll also probably drill a small hole and cover it with an SFD or micropore in my next run of grain jars

I've been doing G2G transfers into grain bags w/ these unmodded lid jars and colonization goes WAY faster once it's in the bag, which has a filter patch, leading me to believe that there is not enough GE in the jars

Thank you for the advice


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