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Invisiblemaxmush
Always learning...

Registered: 06/13/20
Posts: 440
Random plates - LC test, spores and second tranfers
    #26935027 - 09/14/20 11:15 AM (3 years, 4 months ago)

Hey everyone. Been messing around with some agar work and could use some advice.

I have three plates: 1. Mexican Dutch King second transfer from print 2. Space Coast print->syringe->agar 3. Golden Teacher Isolate LC test

Very random. But would love your input. I'll just post them for now and hear your thoughts. Thanks!!

Mex/Dutch



Space Coast



Golden Teacher Isolate LC test



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Disclaimer: all information presented is intended for educational purposes only. All photos are only representations and not directly from the user.


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Invisiblemaxmush
Always learning...

Registered: 06/13/20
Posts: 440
Re: Random plates - LC test, spores and second tranfers [Re: maxmush]
    #26935782 - 09/14/20 07:10 PM (3 years, 4 months ago)

I guess i better pose a question to get responses lol.

Where would you sector these out and how do they look?

I have been having a hard time getting good plates from prints.

The Space Coast one looks crazy and i dont even know where to start?

The Mex/Dutch King T2 still looks weak? Is that normal?

The GT Isolate LC test has some interesting growth so far. Where would you take a transfer from?


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Disclaimer: all information presented is intended for educational purposes only. All photos are only representations and not directly from the user.


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Invisiblemaxmush
Always learning...

Registered: 06/13/20
Posts: 440
Re: Random plates - LC test, spores and second tranfers [Re: maxmush]
    #26938779 - 09/16/20 10:36 AM (3 years, 4 months ago)

last bump...anyone have some thoughts on areas to transfer from?


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Disclaimer: all information presented is intended for educational purposes only. All photos are only representations and not directly from the user.


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Offlinetryptkaloids
Learner
I'm a teapot


Registered: 02/08/15
Posts: 12,641
Loc: Exact Center
Last seen: 2 days, 7 hours
Trusted Cultivator
Re: Random plates - LC test, spores and second tranfers [Re: maxmush]
    #26938820 - 09/16/20 11:09 AM (3 years, 4 months ago)

First two are mold.

Lc test looks like mostly mold


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"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
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Invisiblemaxmush
Always learning...

Registered: 06/13/20
Posts: 440
Re: Random plates - LC test, spores and second tranfers [Re: tryptkaloids]
    #26938932 - 09/16/20 12:23 PM (3 years, 4 months ago)

Ouch really. I thought the LC looked fairly clean?


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Disclaimer: all information presented is intended for educational purposes only. All photos are only representations and not directly from the user.


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Offlinetryptkaloids
Learner
I'm a teapot


Registered: 02/08/15
Posts: 12,641
Loc: Exact Center
Last seen: 2 days, 7 hours
Trusted Cultivator
Re: Random plates - LC test, spores and second tranfers [Re: maxmush]
    #26938939 - 09/16/20 12:35 PM (3 years, 4 months ago)

these spots look like cube myc. The rest is too... ...patchy? Idk if thats the right word.  Should be more uniform


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 


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Offlinejunk_f00d
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Registered: 12/04/15
Posts: 933
Last seen: 1 year, 2 months
Re: Random plates - LC test, spores and second tranfers [Re: tryptkaloids]
    #26939036 - 09/16/20 01:19 PM (3 years, 4 months ago)

LC is so weird looking until it gets a running start, try squirting just a drop in the center next time so you can watch it as it matures. I agree they don't look very good, I would not trust those syringes unless further testing yields better results.


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OfflineA.k.aM
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Registered: 10/27/19
Posts: 16,782
Loc: Gaming the system
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Re: Random plates - LC test, spores and second tranfers [Re: junk_f00d]
    #26939106 - 09/16/20 01:50 PM (3 years, 4 months ago)

Yeah the last one is the only one that looks like it’s got any myc at all but probably not salvageable.


Either your technique or your spores are not great. Was this syringe or streaking a print?


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LAGM2020


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OfflineJatzan
Mycophile
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Registered: 07/21/20
Posts: 148
Last seen: 2 years, 4 months
Re: Random plates - LC test, spores and second tranfers [Re: A.k.a]
    #26939187 - 09/16/20 02:28 PM (3 years, 4 months ago)

Your dishes are so transparent, what agar recipe do you use?


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Invisiblemaxmush
Always learning...

Registered: 06/13/20
Posts: 440
Re: Random plates - LC test, spores and second tranfers [Re: Jatzan]
    #26939723 - 09/16/20 07:58 PM (3 years, 4 months ago)

The mex/dutch is a t2 from a print and the Space Coast was a print->syringe->agar. This was done as straight streaking from print did not germinate after several attempts.

The LC was from a clean plate isolate of GT. I clearly messed up the agar transfer or didnt sterilze the 4% karo enough. This sport takes a lot of patience to get it right :smile: I am going to keep on moving with the help from all you guys . Thanks

BTW Jatzan, these simple PDYA carbon plates.


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Disclaimer: all information presented is intended for educational purposes only. All photos are only representations and not directly from the user.


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