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Offlinemrpharmacist
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Inverting my PF tek jars to complete colonisation?
    #26934831 - 09/14/20 09:49 AM (3 years, 4 months ago)

Second grow (PF Tek).  I inoculated 4 weeks ago into the 400 mL Ball jars.  Two jars are fully colonized, two are almost there (first pic), but the other 7 are going a bit slow and the bottoms of the jars aren't covered yet.  It feels like colonisation has slowed down in the last week.

I've read about inverting the jars and tapping to shift the cake to encourage some air and space into the bottom the jar.  I've done this as shown in the pics.

Do you think this will help?  Any other suggestions?

Btw, the lighting in the pics makes some of the cakes look a bit yellow.  I was using a flashlight.  With the naked eye they're all a nice white colour so fairly sure I'm contamination free. :smile:

Thanks,
Mr P :smile:



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Offlinetryptkaloids
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Re: Inverting my PF tek jars to complete colonisation? [Re: mrpharmacist]
    #26934834 - 09/14/20 09:50 AM (3 years, 4 months ago)

Only one way to know if itll work.


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Re: Inverting my PF tek jars to complete colonisation? [Re: tryptkaloids]
    #26934853 - 09/14/20 09:57 AM (3 years, 4 months ago)

Typically when colonization slows significantly, I point my finger at bacteria. But you are using pf which is notorious for having uncolonized bottoms. That could be because it's too dry of a sub. If it's a little piece left that doesn't seem to want to colonize, I would just take it out and rinse that part off in tap water. But if they slow down halfway through, I would not expect a happy ending.


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Offlinetryptkaloids
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Re: Inverting my PF tek jars to complete colonisation? [Re: Nobler Hino]
    #26934876 - 09/14/20 10:04 AM (3 years, 4 months ago)

Nobler. They are in tall jars. My bet is the bottoms got too dense.


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
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Offlinemrpharmacist
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Re: Inverting my PF tek jars to complete colonisation? [Re: tryptkaloids]
    #26934904 - 09/14/20 10:14 AM (3 years, 4 months ago)

Yeah, I heard the larger mason jars can struggle to fully colonise to the bottom.  Perhaps too dense.  Interestingly, the ones which have fully colonised had a bigger layer of verm at the top.  A good inch or more, so there was less substrate to colonise overall.

Thanks for the replies. If it comes to it I'll birth them in about a week and will rinse off any uncolonised substrate.

Hopefully the invert and tap will encourage the bottoms to finish.  It's all a learning experience...

Will post an update...

cheers,
Mr P :smile:


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Re: Inverting my PF tek jars to complete colonisation? [Re: mrpharmacist]
    #26934910 - 09/14/20 10:16 AM (3 years, 4 months ago)

Your jars were way too wet. They have no oxygen so flipping them over is the only way to start the growth again by introducing some O2 in there. If you wait too long after they stall then it may not work but its worth a try and be patient.


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Offlinemrpharmacist
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Re: Inverting my PF tek jars to complete colonisation? [Re: shroomrookie]
    #26940141 - 09/17/20 05:47 AM (3 years, 4 months ago)

Thanks for the tip and for explaining the logic behind it.  That's kind of how I imagined it.  Since the flip and tap (about 4 days ago) I've seen a few mm of additional myc growth in the direction of the uncolonised area.  So they seem to be responding to the O2 and space.

One or two jars look like no advancement.  Possibly stalled for good.

I'll give them some more time.  Hopefully the creepers will achieve full coverage in another week or two.

Worst case, i'll birth the incomplete jars and rinse off any substrate that didn't colonise before I dunk and roll. 

:o)


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Offlineshroomrookie
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Re: Inverting my PF tek jars to complete colonisation? [Re: mrpharmacist]
    #26941290 - 09/17/20 08:28 PM (3 years, 4 months ago)

Quote:

mrpharmacist said:
Worst case, i'll birth the incomplete jars and rinse off any substrate that didn't colonise before I dunk and roll. 

:o)




I think your right. I would do the same thing if I didn't see the growth pick up.

I know you have considered this but remember that the area you wash off may be more susceptible to contamination but it is the right choice.


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InvisibleZiran
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Re: Inverting my PF tek jars to complete colonisation? [Re: shroomrookie]
    #26941323 - 09/17/20 08:44 PM (3 years, 4 months ago)

Doing that disturbs the dry verm layer. which is your barrier against contaminates.


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Offlineshroomrookie
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Re: Inverting my PF tek jars to complete colonisation? [Re: Ziran]
    #26941357 - 09/17/20 09:01 PM (3 years, 4 months ago)

Inverting them?  Usually if you have to invert them its because the bottom of the jar has not colonized. The top is usually completely colonized by that time and less susceptible to contamination. And it is usually the only chance to get some growth going. It works well if you don't have much left to go and you catch it early.

But using half pint jars for PF tek is better to avoid this stalling.


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Offlinemrpharmacist
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Re: Inverting my PF tek jars to complete colonisation? [Re: shroomrookie]
    #26950612 - 09/23/20 07:45 AM (3 years, 4 months ago)

Thanks for the tip.  Yep, patience has been key.  A good centimetre of extra growth has now occurred since flipping, so most of the jars should be fully colonised within a week.  It seems I just brought them back from the 'stall'.  ;o)

3 are already fully done and are soaking before I roll and put them in the FC tonight. 

Shame they didn't complete colonisation all at the same time, but I'm cool with a staggered harvest. It'll keep me occupied and I have plenty of time. 

It's all learning.

Cheers

Mr P :smile:


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InvisibleMateja
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Re: Inverting my PF tek jars to complete colonisation? [Re: mrpharmacist]
    #26950836 - 09/23/20 10:23 AM (3 years, 4 months ago)

You can also just scrape off what isn't colonized, no point in waiting all the extra time for the last small bit to finish is what I'm thinking :shrug: the substrates will still perform pretty much the same :thumbup:


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Offlinemrpharmacist
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Re: Inverting my PF tek jars to complete colonisation? [Re: Mateja]
    #26961721 - 09/30/20 10:04 AM (3 years, 3 months ago)

Thanks Mateah. You make a good point here.  The waiting game has been lengthy.  Fortunately I wasn't in a hurry.  The jars have fully colonised in batches. The time to fully colonise looks like this:

Inoculated: 10th August

1st jars (3) - 42 days
2nd jar (1) - 46 days
3rd batch (3) - 50 days (Today!)
4th batch (4) - 57 days (estimate 1 more week)

I'm new to this, but it's felt like a lonnnng time!  Thankfully the mycelium looks healthy and the first 3 jars are fruiting nicely, a week after putting them into the FC.

Any estimates on total yield from my 11 x 450 mL jars?  I'm hoping for 90g dried, but I'd be happy with 60!  ;o)

Mr P  :smile:


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Offlinemrpharmacist
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Re: Inverting my PF tek jars to complete colonisation? [Re: mrpharmacist]
    #26970266 - 10/05/20 08:41 AM (3 years, 3 months ago)

It's good to see my cakes are producing much more robust fruit than the first grow.  Definite improvement. 

Btw, most of my inverted cakes caught up and colonised and are now in the FC.  Happy days! :smile:



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