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maxmush
Always learning...

Registered: 06/13/20
Posts: 440
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Contam help (partially color blind). Need another set of eyes
#26933639 - 09/13/20 03:27 PM (3 years, 4 months ago) |
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Hey everyone. Could really use your help. I'm very color blind but i can obviously notice textural differences. I have 3 bags that look very suspect:
Bag 1

Bag 2

Bag 3

All PE with verified clean LC on agar. Inoculated Aug 6. 2 possible vectors: grain and injection technique.
I am terrible with spotting contams. What do you guys think? They look a little wet a mushy in those areas.
Thank you!
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Shquonk
Prof. Mushroom Grower

Registered: 01/02/19
Posts: 127
Last seen: 2 years, 6 months
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Re: Contam help (partially color blind). Need another set of eyes [Re: maxmush]
#26933643 - 09/13/20 03:32 PM (3 years, 4 months ago) |
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Looks like Wetspot bacteria. Sorry bud.
-------------------- Ain't that just the way.
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maxmush
Always learning...

Registered: 06/13/20
Posts: 440
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Re: Contam help (partially color blind). Need another set of eyes [Re: Shquonk]
#26933662 - 09/13/20 03:44 PM (3 years, 4 months ago) |
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Dammit!
Possible causes? Grain too wet to start? Too much LC (3-5cc)? Bad luck?? lol
-------------------- Disclaimer: all information presented is intended for educational purposes only. All photos are only representations and not directly from the user.
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budmanman
OTD Masterbater



Registered: 02/07/07
Posts: 17,975
Loc: PNW
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Re: Contam help (partially color blind). Need another set of eyes [Re: maxmush]
#26933667 - 09/13/20 03:46 PM (3 years, 4 months ago) |
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if it finishes colonizing it will likely still have the bacteria but I have successfully spawned and fruited bacterial jars that looked similar before, as long as they colonize 100%.
Is it ideal? No and any respectable grower would avoid it but I am not very respectable .
-------------------- Everything I have ever said is total bogus bs I am full of crud therefore everything I say should never be taken literal. And I am mentally unstable.
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Edmunter
Mr



Registered: 05/01/13
Posts: 5,699
Last seen: 18 days, 6 hours
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Re: Contam help (partially color blind). Need another set of eyes [Re: budmanman]
#26933685 - 09/13/20 03:56 PM (3 years, 4 months ago) |
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1-2 bacteria and 3 might have a cobweb middle of the clock
Your grains are far too wet. Oats need to be almost too dry or they hold a weird unkillable(it seems) bacteria that always appears. Use millet........
As Bud said ive had spawn that contaminated with bacteria b4 that have fruited. You 100% have to get you substrate right though. Definitely not too wet..
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maxmush
Always learning...

Registered: 06/13/20
Posts: 440
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Re: Contam help (partially color blind). Need another set of eyes [Re: Edmunter]
#26933823 - 09/13/20 05:23 PM (3 years, 4 months ago) |
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Thanks guys. I have had such good luck with oats until now 
Will likely switch to rye, millet, or WBS next time. Oats are just so cheap comparatively but it clearly has downfalls.
How do i avoid this in the future? We are getting an actual autoclave soon. Would this help? These oats were 120min @ 15 psi.
Grains were fairly dry to start. In fact They looked too dry. My sterile tek is fairly good (not perfect), so i suspect its the grains. I guess i'll toss em in the forest.
-------------------- Disclaimer: all information presented is intended for educational purposes only. All photos are only representations and not directly from the user.
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Edmunter
Mr



Registered: 05/01/13
Posts: 5,699
Last seen: 18 days, 6 hours
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Re: Contam help (partially color blind). Need another set of eyes [Re: maxmush]
#26933843 - 09/13/20 05:30 PM (3 years, 4 months ago) |
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No spawn them, u might be lucky
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budmanman
OTD Masterbater



Registered: 02/07/07
Posts: 17,975
Loc: PNW
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Re: Contam help (partially color blind). Need another set of eyes [Re: Edmunter]
#26933853 - 09/13/20 05:36 PM (3 years, 4 months ago) |
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spawn them after 100% colonization, if you get a flush I suggest not going past the 3rd, sure you could but man that substrate will get super soft and liquids by the 4th 5th etc and also it will eventually trich out.
-------------------- Everything I have ever said is total bogus bs I am full of crud therefore everything I say should never be taken literal. And I am mentally unstable.
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hazyhorse
scoobin



Registered: 03/19/19
Posts: 3,820
Last seen: 16 hours, 24 minutes
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Re: Contam help (partially color blind). Need another set of eyes [Re: budmanman]
#26933876 - 09/13/20 05:46 PM (3 years, 4 months ago) |
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donβt be discouraged with oats! they are super easy once you get them down. a solid 30-40 minute boil should be plenty for them. i let my oats dry in the strainer for like two hours, or sometimes iβll put them in the fridge overnight once they cool just to let them dry a bit more
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maxmush
Always learning...

Registered: 06/13/20
Posts: 440
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Re: Contam help (partially color blind). Need another set of eyes [Re: hazyhorse]
#26933916 - 09/13/20 06:03 PM (3 years, 4 months ago) |
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cool guys!
I'll give them a whirl 
I dont want to give up on oats TBH. They are the easiest to acquire and cheapest grains for me.
I have spawned many tubs with them previously with 3-4 flushes no issues. Not sure what happened here? Also been getting pre-inoculation contam as well. Losing about 2% before knocking them up. Might be a bad batch thats in circulation. Looking for race oats at the moment.
-------------------- Disclaimer: all information presented is intended for educational purposes only. All photos are only representations and not directly from the user.
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