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spikopat
Stranger
Registered: 06/28/20
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Why does agar melt in the petri dish?
#26933597 - 09/13/20 02:56 PM (3 years, 4 months ago) |
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Hello sir. I made 12 petri dishes using agar agar on 06.09.2020. I dropped spores with liquid water into petri dishes using a spore syringe tek. Today, 3 of them switched from solid form to gel, jelly-like form and I threw those petri dishes into the bin. What is the reason of this? My room temperature is 79-83F and I store them in the dark.
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Shquonk
Prof. Mushroom Grower

Registered: 01/02/19
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Re: Why does agar melt in the petri dish? [Re: spikopat]
#26933630 - 09/13/20 03:19 PM (3 years, 4 months ago) |
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What was your agar recipe?
-------------------- Ain't that just the way.
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spikopat
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Registered: 06/28/20
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Re: Why does agar melt in the petri dish? [Re: Shquonk]
#26933651 - 09/13/20 03:36 PM (3 years, 4 months ago) |
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250 ML Potato Broth + 3gr agar + 3 gr sugar powder. This is the recipe which i used.

What do you think about theese myceliums, are they healthy? Thanks.
Edited by spikopat (09/13/20 03:50 PM)
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Shquonk
Prof. Mushroom Grower


Registered: 01/02/19
Posts: 127
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Re: Why does agar melt in the petri dish? [Re: spikopat]
#26933672 - 09/13/20 03:50 PM (3 years, 4 months ago) |
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You need more agar. its liquid probably because there just wasnt enough in there to solidify it
-------------------- Ain't that just the way.
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Shquonk
Prof. Mushroom Grower


Registered: 01/02/19
Posts: 127
Last seen: 2 years, 6 months
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Re: Why does agar melt in the petri dish? [Re: spikopat]
#26933675 - 09/13/20 03:51 PM (3 years, 4 months ago) |
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Mycelium looks good in the pics though. kinda hard to tell with the quality
-------------------- Ain't that just the way.
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,809
Loc: Canada
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Re: Why does agar melt in the petri dish? [Re: Shquonk]
#26933691 - 09/13/20 03:59 PM (3 years, 4 months ago) |
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Low pH can cause issues with agar gelling. Also some species of gram negative bacteria can break down agar, reducing it to goo.
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spikopat
Stranger
Registered: 06/28/20
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Re: Why does agar melt in the petri dish? [Re: Pastywhyte]
#26933721 - 09/13/20 04:16 PM (3 years, 4 months ago) |
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Thanks for the comments. Which area do you think I can use to transfer to another petri dish?
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,809
Loc: Canada
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Re: Why does agar melt in the petri dish? [Re: spikopat]
#26933787 - 09/13/20 04:53 PM (3 years, 4 months ago) |
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Take from the more robust and vigorous colony. See how it does when you do. Take lots of transfers and throw most away. Only keep what’s good. If nothing is good start over, don’t waste time chasing stuff that isn’t there.
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spikopat
Stranger
Registered: 06/28/20
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Re: Why does agar melt in the petri dish? [Re: Pastywhyte]
#26947800 - 09/21/20 01:27 PM (3 years, 4 months ago) |
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Sir how can I strain the potato mix? I cannot use a coffee filter because the water does not drip at all. I need some clarity.
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Yumyumyumyum
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Re: Why does agar melt in the petri dish? [Re: Pastywhyte]
#26947842 - 09/21/20 01:54 PM (3 years, 4 months ago) |
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Unless you want to build a contamination collection . I store my busted plates in a case so I can collect every disaster for future reference. All three of those samples look fine the one on the left has good growth.
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