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raoulduke69
Pretty Cool Guy


Registered: 07/12/20
Posts: 19
Last seen: 2 years, 7 months
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Agar Question - When to Refrigerate
#26932554 - 09/12/20 09:36 PM (3 years, 5 months ago) |
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I'm following pastywhite's agar tek and wanted to know one thing (probably a stupid question).
I understand that when sterilizing anything in a PC, growers like to let things cool overnight. That's fine. However, what if I am not going to inoculate my agar plates until 24 hours after sterilization or longer? Could I leave them in room temperature, perhaps in my SAB, or should I refrigerate them?
If I refrigerate them, how long do they need to sit in room temp before I can inoculate?
Thanks in advance for your help and advice.
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hazyhorse
scoobin



Registered: 03/19/19
Posts: 3,821
Last seen: 8 days, 10 minutes
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Re: Agar Question - When to Refrigerate [Re: raoulduke69]
#26932574 - 09/12/20 09:48 PM (3 years, 5 months ago) |
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you donโt need to refrigerate agar plates at all really. unless youโre keeping plates for months theyโll be fine at room temp
also you can take them out of your PC immediately after it cools to normal pressure if you want. i do it all the time. condensation might be a little worse but it clears up in a few days anyways
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Jbets75
Stranger


Registered: 06/21/20
Posts: 97
Loc: Northeast Florida
Last seen: 10 months, 23 days
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Re: Agar Question - When to Refrigerate [Re: hazyhorse]
#26932582 - 09/12/20 09:51 PM (3 years, 5 months ago) |
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Quick question. Iโve been having a good deal of success using MEA for transfers but I canโt get ms syringes to germinate on MEA. Is there a better agar choice for this than MEA ??
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hazyhorse
scoobin



Registered: 03/19/19
Posts: 3,821
Last seen: 8 days, 10 minutes
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Re: Agar Question - When to Refrigerate [Re: Jbets75]
#26932612 - 09/12/20 10:15 PM (3 years, 5 months ago) |
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uhm iโm not sure, i donโt know much about the nuances of agar formulas. iโm sure someone could get you a better answer. as far as general stuff i know, i think spores germinate a little easier on softer/wetter agar so maybe making your mix a little softer may help. iโm sure the actual agar recipe youโre using can make a difference too i just donโt know about it specifically
-------------------- you're not the first to set foot here, just another =================================== i love glass petris & you can too!! posts i constantly refer back to new to mushroom cultivation?? read this!! ===================================
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