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Offlineraoulduke69
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Agar Question - When to Refrigerate
    #26932554 - 09/12/20 09:36 PM (3 years, 5 months ago)

I'm following pastywhite's agar tek and wanted to know one thing (probably a stupid question).

I understand that when sterilizing anything in a PC, growers like to let things cool overnight. That's fine. However, what if I am not going to inoculate my agar plates until 24 hours after sterilization or longer? Could I leave them in room temperature, perhaps in my SAB, or should I refrigerate them?

If I refrigerate them, how long do they need to sit in room temp before I can inoculate?

Thanks in advance for your help and advice.


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Offlinehazyhorse
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Re: Agar Question - When to Refrigerate [Re: raoulduke69]
    #26932574 - 09/12/20 09:48 PM (3 years, 5 months ago)

you donโ€™t need to refrigerate agar plates at all really. unless youโ€™re keeping plates for months theyโ€™ll be fine at room temp

also you can take them out of your PC immediately after it cools to normal pressure if you want. i do it all the time. condensation might be a little worse but it clears up in a few days anyways


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OfflineJbets75
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Re: Agar Question - When to Refrigerate [Re: hazyhorse]
    #26932582 - 09/12/20 09:51 PM (3 years, 5 months ago)

Quick question.  Iโ€™ve been having a good deal of success using MEA for transfers but I canโ€™t get ms syringes to germinate on MEA.  Is there a better agar choice for this than MEA ??


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Offlinehazyhorse
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Re: Agar Question - When to Refrigerate [Re: Jbets75]
    #26932612 - 09/12/20 10:15 PM (3 years, 5 months ago)

uhm iโ€™m not sure, i donโ€™t know much about the nuances of agar formulas. iโ€™m sure someone could get you a better answer. as far as general stuff i know, i think spores germinate a little easier on softer/wetter agar so maybe making your mix a little softer may help. iโ€™m sure the actual agar recipe youโ€™re using can make a difference too i just donโ€™t know about it specifically


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===================================
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new to mushroom cultivation?? read this!!
===================================

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