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OfflineCubePusher
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Practical Storage of Pink Oysters
    #26931736 - 09/12/20 12:25 PM (3 years, 4 months ago)

Hi, sorry for the dumb question but I've used the search function without arriving at an answer I like. I have a culture fridge, but I like to keep it at about 34-35, as this is ideal AFAIK for just about all non-tropical species. I know some people have luck with putting Pinks in the fridge at these temps, but mine died within a week of being in there.

I really don't want TWO fridges, and the thought of just leaving slants at room temp creeps me out. I may try the sterile water method (storing them in sterile water at room temp with no nutrients so cell division is halted). However even that seems like a pain in the butt as getting the culture back out might be difficult since it doesn't spread throughout the liquid like in an LC. If I were to this it'd almost require a proper stir bar or Oster blender to shred the myc up enough. I've actually heard liquid cultures can last awhile unrefridgerated so I may expiriment with that as well, but I doubt it'd work as well.

While having two fridges, one for pinks and one for other species, is kind of a last resort, I do think getting one of those 'wine fridges' with the clear doors might be nice for keeping cultures I'm currently working with but have not yet slanted for more permanent storage, in addition to keeping master slants of the pinks. I'd probably keep this in the same room as my hood.

Just some thoughts, thanks for reading and please let me know what's worked for you!


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Offlinesendmehummus
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Re: Practical Storage of Pink Oysters [Re: CubePusher]
    #26931886 - 09/12/20 01:54 PM (3 years, 4 months ago)

I've had blue oyster LC I made 3 months ago sitting on a shelf at room temp. Tested it last week on agar and it's still clean. I'm sure it can go way longer than even that but I'm gonna be using it real soon. My LC jar lids are the same as my grain jars (silicone port and poly filter) but I also cover the tops with tinfoil when they are gonna sit a long time. I don't use the poly so the myc can breathe for LC, but to avoid using a filter syringe to relieve negative pressure. That's my setup, seems to have been working great so far 👍.


--------------------
Be patient and keep it simple.


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Offlinevsoares
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Re: Practical Storage of Pink Oysters [Re: sendmehummus]
    #26933278 - 09/13/20 11:41 AM (3 years, 4 months ago)

Yeah pinks are problematic to store. I think your best shot is growing them on a low nutrition agar plate, then peeling off the top growth, making sure not to pick any agar, and putting it in a small flask with distilled sterilized water. This is a known tek, i dont remember how ppl call It though.

What i myself do is to always grow at least a couple of blocks of pinks, and when i have no more spawn and my plates are old i Will clone back to a New agar plate. Ive been doing that for the last two years.


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OfflineCubePusher
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Re: Practical Storage of Pink Oysters [Re: vsoares]
    #26933456 - 09/13/20 01:14 PM (3 years, 4 months ago)

So you guys don't like my wine fridge idea?

Quote:

sendmehummus said:
I've had blue oyster LC I made 3 months ago sitting on a shelf at room temp. Tested it last week on agar and it's still clean. I'm sure it can go way longer than even that but I'm gonna be using it real soon. My LC jar lids are the same as my grain jars (silicone port and poly filter) but I also cover the tops with tinfoil when they are gonna sit a long time. I don't use the poly so the myc can breathe for LC, but to avoid using a filter syringe to relieve negative pressure. That's my setup, seems to have been working great so far 👍.



Hmmm, I'll have to remember to shoot you a message in 6-12 months and check up on that LC if it's still hanging around. At 3 months, that's only slightly better than an agar plate IMO, but I do want to test out the longevitiy of LCs a bit over the next couple years.

Quote:

vsoares said:
Yeah pinks are problematic to store. I think your best shot is growing them on a low nutrition agar plate, then peeling off the top growth, making sure not to pick any agar, and putting it in a small flask with distilled sterilized water. This is a known tek, i dont remember how ppl call It though.

What i myself do is to always grow at least a couple of blocks of pinks, and when i have no more spawn and my plates are old i Will clone back to a New agar plate. Ive been doing that for the last two years.




I'm definately going to try this (why not), but I'm going to ad an entire plate to a jar with a stir bar, in hopes of shredding the myc finely enough that I can just pour or squirt it like an LC down the road. Might be cool to actually suck it up in a syringe for easy reculturing later. I think the tek is called 'Distilled Water Tek' or something like that btw, not sure either though.

You don't experience degradation of your culture by repeatedly running out cultures and then just cloning? I've never heard of anyone doing that before.


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OfflinePowfuu
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Re: Practical Storage of Pink Oysters [Re: CubePusher]
    #26933724 - 09/13/20 04:17 PM (3 years, 4 months ago)

I have been looking for an answer on this same topic. I think making liquid culture is the way to go for pinks??
I have not made LC though, is it that simple as to throw the agar culture in a jar with steriled water?? I will have to read up on some teks for it.


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OfflineForresterM
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Re: Practical Storage of Pink Oysters [Re: Powfuu]
    #26933827 - 09/13/20 05:24 PM (3 years, 4 months ago)

Quote:

Powfuu said:
I have been looking for an answer on this same topic. I think making liquid culture is the way to go for pinks??
I have not made LC though, is it that simple as to throw the agar culture in a jar with steriled water?? I will have to read up on some teks for it.




Liquid culture and distilled water storage of cultures are two different things entirely, so just know that when you're researching.


--------------------
Repugnant is a creature who would squander the ability to lift an eye to heaven, conscious of his fleeting time here.
-------------------

Have some medicinal mushrooms and want to get the most out of them?  Try this double extraction method.


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OfflineCubePusher
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Re: Practical Storage of Pink Oysters [Re: Powfuu]
    #26933828 - 09/13/20 05:25 PM (3 years, 4 months ago)

Quote:

Powfuu said:
I have been looking for an answer on this same topic. I think making liquid culture is the way to go for pinks??
I have not made LC though, is it that simple as to throw the agar culture in a jar with steriled water?? I will have to read up on some teks for it.




There is LI (liquid innoculant) and LC (liquid culture). LI refers to the agar in sterile water, LC refers to innoculating a nutrient broth so that the myc colonizes the whole thing and you can then inoculate with just a drop of the liquid.

For culture storage, there have been test showing good results on the longevity of LI, comparable to slants if not greater, but I don't know about the longevitiy of LC. I agree it (LI) is probably a good method for pinks, I'm also still considering the wine cooler fridge idea for the lab since the temps sit much higher in those.

Beautiful flush of pinks in that other thread btw!


Edited by CubePusher (09/13/20 05:36 PM)


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OfflineForresterM
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Re: Practical Storage of Pink Oysters [Re: CubePusher]
    #26933858 - 09/13/20 05:38 PM (3 years, 4 months ago)

LC will last quite a while it just gets difficult to get through a syringe.  Don't think it's the ideal method for culture storage though...


--------------------
Repugnant is a creature who would squander the ability to lift an eye to heaven, conscious of his fleeting time here.
-------------------

Have some medicinal mushrooms and want to get the most out of them?  Try this double extraction method.


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OfflinePowfuu
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Re: Practical Storage of Pink Oysters [Re: Forrester]
    #26934716 - 09/14/20 08:36 AM (3 years, 4 months ago)

Quote:

Forrester said:
Quote:

Powfuu said:
I have been looking for an answer on this same topic. I think making liquid culture is the way to go for pinks??
I have not made LC though, is it that simple as to throw the agar culture in a jar with steriled water?? I will have to read up on some teks for it.




Liquid culture and distilled water storage of cultures are two different things entirely, so just know that when you're researching.




Thank you for making that clear for me. This week I will be doing alot of research on both of them, reading threads as well as videos.


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OfflinePowfuu
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Re: Practical Storage of Pink Oysters [Re: CubePusher]
    #26934721 - 09/14/20 08:40 AM (3 years, 4 months ago)

Quote:

CubePusher said:
Quote:

Powfuu said:
I have been looking for an answer on this same topic. I think making liquid culture is the way to go for pinks??
I have not made LC though, is it that simple as to throw the agar culture in a jar with steriled water?? I will have to read up on some teks for it.




There is LI (liquid innoculant) and LC (liquid culture). LI refers to the agar in sterile water, LC refers to innoculating a nutrient broth so that the myc colonizes the whole thing and you can then inoculate with just a drop of the liquid.

For culture storage, there have been test showing good results on the longevity of LI, comparable to slants if not greater, but I don't know about the longevitiy of LC. I agree it (LI) is probably a good method for pinks, I'm also still considering the wine cooler fridge idea for the lab since the temps sit much higher in those.

Beautiful flush of pinks in that other thread btw!





Thank you for explaining the difference in both of those methods for me! Just from hearing this from both of you two, I'm thinking LI is the way to go for long term storage of the pinks. I would also like to try LC.. I will probably end up learning both methods, ill try storing some for long term in LI, and making a couple LC's, as I could use them that month maybe.

With this info I will be doing some research.
Thank you btw on the compliment on my pinks!🙏🏼


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OfflineCubePusher
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Re: Practical Storage of Pink Oysters [Re: Powfuu]
    #26935587 - 09/14/20 05:05 PM (3 years, 4 months ago)

Yep, the more tools you add to your toolbelt the better! I put off learning LC for a long time but think it has a lot of utility now (it's basically a more scalable version of agar to grain transfer, but requires you to check for contams before expanding like a madman otherwise you're taking a huge risk when nocc'ing up grain.

Sounds like we'll both be doing the same stuff with our pinks, good luck! If you ever want to trade cultures or something let me know, super jealous of your flushes.


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OfflineForresterM
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Re: Practical Storage of Pink Oysters [Re: CubePusher]
    #26935606 - 09/14/20 05:14 PM (3 years, 4 months ago)

I find LC to be invaluable when working with edibles.  Much easier than agar wedges.  Plus you can inoculate grain in open air which saves a shitload of time :thumbup:


--------------------
Repugnant is a creature who would squander the ability to lift an eye to heaven, conscious of his fleeting time here.
-------------------

Have some medicinal mushrooms and want to get the most out of them?  Try this double extraction method.


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Re: Practical Storage of Pink Oysters [Re: Forrester]
    #26935672 - 09/14/20 05:56 PM (3 years, 4 months ago)

That's what got me into it too. Though I still haven't put my syringes to use, I've just been free pouring. I've begun to enjoy free pouring and it saves me from modifying lids and needing syringes, which are now gathering dust.. To check for cleanliness of the culture you can just use a loop dipped then streaked onto a plate (though I haven't done it yet). That method always kinda bugs me though because then how do I know if it contam'd while I stuck my loop in? Then I think about stupid shit like pouring the LC over my loop or something instead. I was kinda thinking of buying these https://www.amazon.com/PYREX-250mL-Round-Storage-Bottles/dp/B004XR5TFM/ref=sr_1_9?dchild=1&keywords=250ml+media+bottle&qid=1600015657&sr=8-9 to get a more precise pour so that maybe I could pour straight onto a plate, just a drop. No idea if it'd work well or not though. Might have to make a thread in mushcult if I can't find one.

Off topic but I don't really know where I stand on the whole 'a syringe is just another contam vector' topic. Obviously free pouring into a crumbly bag is a vector too, as is opening the lid to the LC container. I guess I'm just saying it's not so black and white.


Edited by junk_f00d (09/14/20 06:14 PM)


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OfflineForresterM
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Re: Practical Storage of Pink Oysters [Re: junk_f00d]
    #26936302 - 09/15/20 02:42 AM (3 years, 4 months ago)

That's why I like syringes, you can squirt as much or little out as you want, even in open air, without contaminating the syringe (just flame it).  For testing, innoculating, in jars, bags, or whatever.  All you have to do is make sure the LC going into it is 100% clean then it's a free for all after that.

Modifying lids is worth the effort to me to be able to do that.  They last long enough for the little time you put into em. 

I may be one of few, but I'm for syringes all the way!


--------------------
Repugnant is a creature who would squander the ability to lift an eye to heaven, conscious of his fleeting time here.
-------------------

Have some medicinal mushrooms and want to get the most out of them?  Try this double extraction method.


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