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OfflinePsylofan90
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Please help! What type of contamination?!
    #26930665 - 09/11/20 07:53 PM (3 years, 5 months ago)

I just posted a much more thorough post under “Mushroom Cultivation” and am wondering what type of contamination this is. This is 2-3 days worth of growth and the pins are growing steadily but Super slow and also have grayish caps. I am pretty sure it is Verticillium but I’ve heard you can still produce fruits. There were birthed and put into 2L bottles with wet perlite and Polyfil on the tops but we’re kept in a closet with the room temp at 70° and humidity was 50%. I decided to put them into my SGFC today (which was originally made for 6 BRF jars and 2 multi grain down bags) to give them more room and also get them out of the closet. The temp stays at 70° and the humidity is at 82% and rising. There are also 1/4” holes drilled every 2” and 5 1/3” of damp perlite. There is a 6500k light hanging above them on a 12/12 cycle and it is set in the corner of the room about 2 1/2 feet off the ground. Here are some pics of the cakes from the first day or pinning 9/8 to today 9/11:



The cakes were birthed and dunked on 9/4, rinsed and rolled on 9/5 and I saw my first pin on 9/8. So far it has taken them 3 days to show the first pin and now there are pins literally popping up every single night. Only problem is the slow growth and weird color and shape to the pins. I’m hoping that moving them into the SGFC will give the more FAE and increase the humidity drastically. I know a 105qt SGFC is absurd for 5 cakes but I work from home and it is in the room right next to me. I will be able to mist/fan or do whatever is needed to them whenever I want. I’m sure it will be hard to dial in but it has to be a better situation than the 2L that was stuck in a closet with practically no FAE and a humidity level that was at half of what it should be at.

Please let me know what I can do to save these cakes and possible get the pins to mature and fruit!


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Offlinerickomalley238
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Re: Please help! What type of contamination?! [Re: Psylofan90]
    #26930670 - 09/11/20 07:57 PM (3 years, 5 months ago)

I think that's normal.


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OfflinePsylofan90
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Re: Please help! What type of contamination?! [Re: Psylofan90]
    #26930671 - 09/11/20 07:57 PM (3 years, 5 months ago)

Here are some pics of them colonizing. It took a total of about 3 weeks for them for fully colonize and then I let it sit for another week and a half to consolidate. They have been in the fruiting stage for 6 days total and pins only showed up 3 days ago with more and more showing up every single day.



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This has been one long, strange ride



Just a regular guy trying to do cool things


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OfflineMycobro420
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Re: Please help! What type of contamination?! [Re: Psylofan90]
    #26930677 - 09/11/20 07:59 PM (3 years, 5 months ago)

those are pins man


Edited by Mycobro420 (09/11/20 08:00 PM)


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OfflinePsylofan90
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Re: Please help! What type of contamination?! [Re: Mycobro420]
    #26930702 - 09/11/20 08:15 PM (3 years, 5 months ago)

Haha I know what they are but the strain is B+ Cubensis and pictures of peoples pins using the drain strain look completely different. None of the caps looked like this (grayish) and also they are growing Super slow. That’s 3 days worth of growth in those pics. Hopefully by taking them out of the bottles that were stuck in the closet with practically no FAE and humidity at 50% it will help them grow. Temp in the SGFC are the same (70°) but the humidity is now at 85% and FAE should not be efficient


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This has been one long, strange ride



Just a regular guy trying to do cool things


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Offlineblindsighted
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Re: Please help! What type of contamination?! [Re: Psylofan90]
    #26930774 - 09/11/20 08:56 PM (3 years, 5 months ago)

They are only gray because of the thin film of mycelium covering the cap from its primordia stage. Once it grows out a lil bit, it should change to normal color.


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OfflinePsylofan90
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Re: Please help! What type of contamination?! [Re: blindsighted]
    #26931000 - 09/11/20 11:43 PM (3 years, 5 months ago)

I know about the Universal Veil and how it leaves parts of it on the mushroom pins when it breaks but this does not look like that at all. The pins are short/fat, growing very slowly, grayish on the tops, growing at weird angles and I also noticed in one of the pics that there are a few pins with a brown stripe on the stems. Something else is going on here because every picture I’ve seen of B+ Cubensis pins looks Very different than mine and they also are growing much faster than mine. I put them into the 2L bottles 7 days ago, saw the first few pins 4 days ago and they are still this small. They are still growing and it seems like the pins almost double in numbers every 24 hours but most people say that their pins grow to mature mushrooms within a few days (3-7) time. Mine grow about 1/4-1/3 of their size each day. It would take nearly a month to get to the size they are supposed to be growing at that rate. I just want someone with experience to tell me what possible contam this is and what I can do to stop it and expedite this growing process


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This has been one long, strange ride



Just a regular guy trying to do cool things


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Offlinerickomalley238
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Re: Please help! What type of contamination?! [Re: Psylofan90]
    #26931012 - 09/12/20 12:14 AM (3 years, 5 months ago)

Quote:

Psylofan90 said:
I know about the Universal Veil and how it leaves parts of it on the mushroom pins when it breaks but this does not look like that at all. The pins are short/fat, growing very slowly, grayish on the tops, growing at weird angles and I also noticed in one of the pics that there are a few pins with a brown stripe on the stems. Something else is going on here because every picture I’ve seen of B+ Cubensis pins looks Very different than mine and they also are growing much faster than mine. I put them into the 2L bottles 7 days ago, saw the first few pins 4 days ago and they are still this small. They are still growing and it seems like the pins almost double in numbers every 24 hours but most people say that their pins grow to mature mushrooms within a few days (3-7) time. Mine grow about 1/4-1/3 of their size each day. It would take nearly a month to get to the size they are supposed to be growing at that rate. I just want someone with experience to tell me what possible contam this is and what I can do to stop it and expedite this growing process




Relax man mine were doing the same exact thing up until a day ago and now I see beautiful healthy mushrooms growing. You can't do anything if it's out of your control anyways can ya? Make sure you are giving them enough water and do your job and be patient, let them work and don't mess with them.


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OfflinePsylofan90
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Re: Please help! What type of contamination?! [Re: rickomalley238]
    #26931033 - 09/12/20 12:28 AM (3 years, 5 months ago)

I’m trying to and that’s why I’m asking questions. Verticillium is a contam that nobody ever really talks about and there isn’t very much information about it out there. If you type it into google and then search for pics on mushrooms you get hundreds of pictures that all look complete different. I’ve seen plenty of pictures that look like my pins and half say it IS infected and half say it ISN'T. Nobody can ever give a straight answer and if it isVerticillium than I have read that you need to Decrease humidity, increase FAE and stay away from misting because it travels extremely well when it is in water. All I need is for someone to let me know what’s up so I can either sit back and keep doing what I have been doing OR stop misting them to prevent the spread and fan the shit out of them


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This has been one long, strange ride



Just a regular guy trying to do cool things


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Offlinerickomalley238
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Re: Please help! What type of contamination?! [Re: Psylofan90]
    #26931044 - 09/12/20 12:36 AM (3 years, 5 months ago)

Quote:

Psylofan90 said:
I’m trying to and that’s why I’m asking questions. Verticillium is a contam that nobody ever really talks about and there isn’t very much information about it out there. If you type it into google and then search for pics on mushrooms you get hundreds of pictures that all look complete different. I’ve seen plenty of pictures that look like my pins and half say it IS infected and half say it ISN'T. Nobody can ever give a straight answer and if it isVerticillium than I have read that you need to Decrease humidity, increase FAE and stay away from misting because it travels extremely well when it is in water. All I need is for someone to let me know what’s up so I can either sit back and keep doing what I have been doing OR stop misting them to prevent the spread and fan the shit out of them




For starters, you should have your cakes on foil, or something so it's not directly on the perlite; the perlite absorbs the water out of the cakes. Secondly, you want your cakes to be closer in proximity to create a microclimate.

I do not think anyone can say if it's bacterial, and if it doesn't smell it's fine. Given the issues listed above, I would suggest lack of water is the issue.


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OfflinePsylofan90
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Re: Please help! What type of contamination?! [Re: rickomalley238]
    #26931049 - 09/12/20 12:41 AM (3 years, 5 months ago)

They are sitting on foil. The pieces are pretty small, about the same size of the cakes. I’m about to cut some new pieces and put them on them


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This has been one long, strange ride



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Offlinerickomalley238
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Re: Please help! What type of contamination?! [Re: Psylofan90]
    #26931052 - 09/12/20 12:43 AM (3 years, 5 months ago)

Quote:

Psylofan90 said:
They are sitting on foil. The pieces are pretty small, about the same size of the cakes. I’m about to cut some new pieces and put them on them




Yes maybe slightly larger because you do not want any direct contact with perlite or it will absorb lots of moisture. Also, a SGFC is meant for lots of cakes for a good microclimate, especially one your size, I personally had little cakes like you, so I made sure my cakes were very close together (2" or so). The cakes look dry as well, make sure you are misting to glistening standards.


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OfflinePsylofan90
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Re: Please help! What type of contamination?! [Re: rickomalley238]
    #26931543 - 09/12/20 10:24 AM (3 years, 5 months ago)

That’s a problem I’m having. I’m not sure if my cakes are dry or if they are soaking wet. I have pics on my phone that show what they are supposed to look like before and after misting but I still don’t get it. I’m probably either starving the cakes or completely drowning them and have no idea which one it is


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This has been one long, strange ride



Just a regular guy trying to do cool things


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