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ataribravo
Magic Myc

Registered: 08/07/20
Posts: 75
Last seen: 2 years, 10 months
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The more I read the more stupid I feel...
#26930736 - 09/11/20 08:33 PM (3 years, 4 months ago) |
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I've been researching LI for using in spawn bags. I guess I've been steered to the non-blended Tek. I ran across a thread talking about petris and that refrigerated dishes may cause a problem. Also, something I've never read about not saw in any video until today, the difference between spore plates and transfer plates.
For 3 years I have been inoculating petris with spore syringes and then placing the mycelium grown on the plate right into spawn bags. And it has worked. So I guess I have 3 questions:
1. Do you or do you not have to use transfer plates for spawn, and why? 2. If you can't use a spore plate directly, why? 3. Can you, should you use refrigerated petris for spawn and do you need to let them warm up and for how long?
I'm really trying to educate myself so I can be successful and, later, pass on knowledge to newer newbs.
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lateforthafuture
fanatic


Registered: 03/26/07
Posts: 471
Last seen: 3 years, 4 months
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Re: The more I read the more stupid I feel... [Re: ataribravo] 1
#26930754 - 09/11/20 08:47 PM (3 years, 4 months ago) |
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Quote:
ataribravo said: I've been researching LI for using in spawn bags. I guess I've been steered to the non-blended Tek. I ran across a thread talking about petris and that refrigerated dishes may cause a problem. Also, something I've never read about not saw in any video until today, the difference between spore plates and transfer plates.
For 3 years I have been inoculating petris with spore syringes and then placing the mycelium grown on the plate right into spawn bags. And it has worked. So I guess I have 3 questions:
1. Do you or do you not have to use transfer plates for spawn, and why? 2. If you can't use a spore plate directly, why? 3. Can you, should you use refrigerated petris for spawn and do you need to let them warm up and for how long?
I'm really trying to educate myself so I can be successful and, later, pass on knowledge to newer newbs.
1. Do you or do you not have to use transfer plates for spawn, and why?
It is best practice to make transfers from the germ plates because prints and syringes are inherently dirty. Can you get clean germ plates....yes...it does happen...but I always make at least one...or as many transfers necessary to where I'm confident that the culture is clean.
2. If you can't use a spore plate directly, why?
Fruiting is generally not done in a sterile environment. Therefore, prints and syringes are not always clean...we make transfers to get the clean healthy mycelium away from any potential contaminates they may have been introduced via syringe or print.
3. Can you, should you use refrigerated petris for spawn and do you need to let them warm up and for how long?
When inoculating spawn I would suggest using fresh plates that are not 100% colonized. I personally wouldn't pull a stored plate straight out of the fridge and inoculate a master jar with it. I would take a wedge to a new plate, evaluate the growth, transfer if necessary, then inoculate.
-------------------- "Not what could have been, or what should have been, but what will be" -Self
 
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