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Offlinefraki58
Mycology Learner

Registered: 11/20/19
Posts: 245
Last seen: 5 days, 10 hours
Help identifying mycelium
    #26930129 - 09/11/20 02:43 PM (3 years, 4 months ago)

Hey guys, so i started doing agar for the first time so I'm not sure if this grow on my plate is indeed mycelium. I inoculated the plates 1 and a half week ago, currently growing pretty slow cause the low temperatures in my area. Hope the pics are detailed enough! 1 pic from the top and one from the bottom with light shining through.





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If any of the said above is incorrect, please correct me! Still learning here and constructive criticism is the best :smile:


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OfflineGan
Wielder of Narya
Male


Registered: 08/26/19
Posts: 927
Loc: Valinor
Last seen: 5 months, 27 days
Re: Help identifying mycelium [Re: fraki58]
    #26930228 - 09/11/20 03:26 PM (3 years, 4 months ago)

Is all of that sediment? Just wanna make sure it is not something on the surface of the plate.

Honestly, I cant say for sure due to the lighting. Snap another pic in better lighting. A little brighter and whiter light if possible.


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Offlinefraki58
Mycology Learner

Registered: 11/20/19
Posts: 245
Last seen: 5 days, 10 hours
Re: Help identifying mycelium [Re: Gan]
    #26930264 - 09/11/20 03:47 PM (3 years, 4 months ago)

Yeah it's sediment, i got this ones, what do you think?







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If any of the said above is incorrect, please correct me! Still learning here and constructive criticism is the best :smile:


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OfflineGan
Wielder of Narya
Male


Registered: 08/26/19
Posts: 927
Loc: Valinor
Last seen: 5 months, 27 days
Re: Help identifying mycelium [Re: fraki58]
    #26930714 - 09/11/20 08:22 PM (3 years, 4 months ago)

That doesnt look like myc to me. But keep that plate and watch it the next couple days or so. Looks like you also have a little satellite colony of something. Walk me through your process of inoculating your dish


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Offlinefraki58
Mycology Learner

Registered: 11/20/19
Posts: 245
Last seen: 5 days, 10 hours
Re: Help identifying mycelium [Re: Gan]
    #26931803 - 09/12/20 01:07 PM (3 years, 4 months ago)

I followed Bods agar tek. Maked a SAB, poured the dishes, let them cool and then i inoculated them with a homemade loop directly from the print. I scratched the print with the loop and did a streak pattern, all of this trying to stay as sterile as possible, this be flaming the loop in between inoculations, trying to not open the dish as much when doing the pouring and inoc,etc. For the agar i used Brf agar.


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If any of the said above is incorrect, please correct me! Still learning here and constructive criticism is the best :smile:


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