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Offlinebenis12
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Agar Plates - No Experience with Tomentose Growth
    #26905575 - 08/29/20 07:34 AM (3 years, 5 months ago)

Typically, I put to grain only plates that I've transferred to look like perfect snowflakes; beautiful little rhyzomorphic specimens.  However, lately the few cultures that I'm working with are not rhyzomorphic at all, they tend to be tomentose growth, even after many transfers.

I've attached four pictures below of two separate PE cultures. I believe they show disorganized but (at least to my eye) contaminant-free tomentose growth.  All are on their 10th-ish transfer.

Would these be fine to drop onto grain? Uncertain on what to do since they don't look like my usual rhyzomorphic plates.  Thanks in advance

Culture 1


Culture 1 (different view)


Culture 2


Culture 2 (different view)


Edited by benis12 (08/29/20 08:08 AM)


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OfflineA.k.aM
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Re: Agar Plates - Tomentose vs Rhyzomorphic [Re: benis12]
    #26905623 - 08/29/20 08:03 AM (3 years, 5 months ago)

Tomentose or rhizo doesn’t matter, just as long as it’s clean.

I would definitely not use the first one.


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Edited by A.k.a (08/29/20 08:04 AM)


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Offlinebenis12
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Re: Agar Plates - Tomentose vs Rhyzomorphic [Re: A.k.a]
    #26905626 - 08/29/20 08:06 AM (3 years, 5 months ago)

Thanks for the reply!

I understand that that's the mantra around here (tomentose or rhyzo, as long as its clean), but do you personally have any experience with the growth you're seeing above?  What do you think of the plates?  They just look so different than what I've always used that I'm not even sure if they're clean or not.

Edit: just saw your edited reply.  What about the first one worries you?


Edited by benis12 (08/29/20 08:07 AM)


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OfflineA.k.aM
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Re: Agar Plates - Tomentose vs Rhyzomorphic [Re: benis12]
    #26905631 - 08/29/20 08:11 AM (3 years, 5 months ago)

I’ve run all kinds of myc and had good results, never really paid attention to what type fruited what way though.



That first one I don’t like the swirls and patterns in it. Something weird is going on unless maybe the agar is lumpy.


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Offlinebenis12
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Re: Agar Plates - Tomentose vs Rhyzomorphic [Re: A.k.a]
    #26905642 - 08/29/20 08:16 AM (3 years, 5 months ago)

Man, I just love looking at rhyzo plates!  Those are some really nice ones, what an interesting selection. Thanks for sharing.

The agar in my plates is flat, so no issues with lumps. I can see some problems with the first one now though, maybe fighting some hidden contams.

In your pics, the third from the left and the farthest right are definitely what mine have been looking like recently.  Cool to know you don't worry about only dropping rhyzos to grain.  I think I've just been so worried about wasting months on what could end up in failure that I've been too scared to try.


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OfflineA.k.aM
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Re: Agar Plates - Tomentose vs Rhyzomorphic [Re: benis12]
    #26905649 - 08/29/20 08:21 AM (3 years, 5 months ago)

When I first got into agar I messed around with recipes and how thick the plates were poured and found out a lot of myc is pretty easy to manipulate into rhizo. So i figure if the same culture can be changed to rhizo or tomentose then it doesn’t really matter.

I think rhizo is mostly selected because it grows quicker.


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Offlinebenis12
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Re: Agar Plates - Tomentose vs Rhyzomorphic [Re: A.k.a]
    #26905659 - 08/29/20 08:26 AM (3 years, 5 months ago)

Makes sense.

Funny - that's exactly what has happened in those plates above.  Two transfers ago, they used to be rhyzos.  So I figured maybe something was happening due to contams.


Edited by benis12 (08/29/20 08:26 AM)


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OfflineA.k.aM
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Re: Agar Plates - Tomentose vs Rhyzomorphic [Re: benis12]
    #26905714 - 08/29/20 09:18 AM (3 years, 5 months ago)

It’s pretty common to have them smooth out or go more rhizo as you do more transfers. Some are crazy though, when I did aa+ the plates were huge puffballs until like t4 then started to look normal.


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Offlinebenis12
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Re: Agar Plates - Tomentose vs Rhyzomorphic [Re: A.k.a]
    #26905731 - 08/29/20 09:29 AM (3 years, 5 months ago)

I only work with PE and I've been getting weird streak-like patterns on plates.  Shapes that resemble ninja stars, basically.  Super bizarre


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Re: Agar Plates - Tomentose vs Rhyzomorphic [Re: benis12]
    #26905850 - 08/29/20 11:11 AM (3 years, 5 months ago)

Like this?



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Re: Agar Plates - Tomentose vs Rhyzomorphic [Re: A.k.a]
    #26905862 - 08/29/20 11:20 AM (3 years, 5 months ago)

My plates did that exact same thing, pesa as well. What is it?

Edit: I cant remember if I transferred away from that odd growth to more uniform or not but im pretty sure.


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Re: Agar Plates - Tomentose vs Rhyzomorphic [Re: Quadryder]
    #26906163 - 08/29/20 02:44 PM (3 years, 5 months ago)

I have no idea but it was really annoying. I’ve had other plates where randomly one or two tiny pizza shaped wedges looked empty but pesa took it to another level.

One of the shoeboxes I ran colonized really well and looked great but took I think 32 days to pin.


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Offlinebenis12
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Re: Agar Plates - Tomentose vs Rhyzomorphic [Re: A.k.a]
    #26928949 - 09/10/20 08:04 PM (3 years, 5 months ago)

Sorry, realize I'm two weeks late to the party but yes, exactly like that.  Those ninja starts are fucking weird...

Goddamn this hobby gets bizarre at times.  Just when I think I've got it all figured out, I realize I don't :smile:


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Offlineverum subsequentis
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Re: Agar Plates - Tomentose vs Rhyzomorphic [Re: benis12]
    #26929129 - 09/10/20 10:13 PM (3 years, 5 months ago)

I think (but am not positive) that that's a lurking contam. Most probably a weak bacterial colony, that causes plates to do that.

OP I don't like the first plate at all. the second was most probably ok before you opened it in what appears to be a non sterile environment.


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