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Offlineseand04
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Mixed spores on agar * 1
    #26929060 - 09/10/20 09:27 PM (3 years, 4 months ago)

I was lucky enough to get an AA+ print and a golden halo print.  I was wondering if I grew them both out separate on agar then took a small cutting off each and put those together on agar if they would grow together.  Like an albino golden spore🤔.


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Offlinelacma50
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Re: Mixed spores on agar [Re: seand04] * 1
    #26929943 - 09/11/20 12:44 PM (3 years, 4 months ago)

I'd imagine if you mixed both spores together and streaked em out on agar, allowed formation of mycelium, and then grew out isolated mycelial cultures from your plates before you had a lawn then you'd eventually come across a hybrid of the two that exhibited the characteristics you're looking for. However, I can't imagine you'd have any way of knowing what's hybridized and what isn't prior to actually seeing the fruiting bodies and so it'd really be a crapshoot.

If you look at a generalized basidio life cycle, the haploid mycelial growth phase (the phase prior to plasmogamy in the picture)is rather short lived, and so by the time you're seeing growth on your plates the mycelium you're looking at is diploid meaning mating has already occurred. So if you were to introduce mycelial isolates from two different plates onto the same plate, what you're doing is really just putting two individual organisms together that have already mated and no longer can.

Now if you were to follow through with the experiment you proposed, there may be a way to weaken cell walls at the junction of the two cultures which may then result in hybridization, however I don't have much knowledge on that subject.

I'm sure this topic has been talked about ad nauseam in the past.


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Offlineseand04
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Re: Mixed spores on agar [Re: lacma50]
    #26930601 - 09/11/20 07:06 PM (3 years, 4 months ago)

Thanks a lot for this information.  I have been looking around but I haven't found much on this subject.


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InvisiblePastywhyteMDiscord
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Re: Mixed spores on agar [Re: seand04] * 2
    #26953962 - 09/25/20 07:02 AM (3 years, 4 months ago)

Mixing spores works best when the samples are both fresh, that way you can have the best chance for simultaneous germination.


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Offlineseand04
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Re: Mixed spores on agar [Re: Pastywhyte] * 1
    #26954639 - 09/25/20 03:02 PM (3 years, 4 months ago)

Awesome thanks pasty. What about if you made a print of one, then took another print right on top, then to agar?


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Re: Mixed spores on agar [Re: seand04]
    #26954986 - 09/25/20 07:27 PM (3 years, 4 months ago)

Quote:

seand04 said:
Awesome thanks pasty. What about if you made a print of one, then took another print right on top, then to agar?



That would likely work.


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Offlineseand04
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Re: Mixed spores on agar [Re: Pastywhyte] * 1
    #26955169 - 09/25/20 10:01 PM (3 years, 4 months ago)

Gonna do it.  I have GH and AA+ growing right now.


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Re: Mixed spores on agar [Re: seand04]
    #27196591 - 02/10/21 12:32 AM (2 years, 11 months ago)

Quote:

seand04 said:
Gonna do it.  I have GH and AA+ growing right now.




Did you get anything cool?


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Offlineseand04
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Re: Mixed spores on agar [Re: nektar61] * 1
    #27197248 - 02/10/21 11:54 AM (2 years, 11 months ago)

I have some golden halo spores and penis envy germinating on agar right now.


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OfflineNichrome
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Re: Mixed spores on agar [Re: seand04] * 1
    #27200641 - 02/11/21 11:13 PM (2 years, 11 months ago)

Rad! Can't wait to see what happens.


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OnlineLogicaL ChaosM
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Re: Mixed spores on agar [Re: seand04]
    #27204286 - 02/13/21 06:19 PM (2 years, 11 months ago)

Strain hybridization via spore printing eh? Very cool :takingnotes:


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Offlineseand04
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Re: Mixed spores on agar [Re: LogicaL Chaos] * 2
    #27205579 - 02/14/21 01:50 PM (2 years, 11 months ago)

Yes sir. Here's one of the agar plates 2nd transfer Golden halo and PE spore mix.


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Offlineseand04
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Re: Mixed spores on agar [Re: seand04]
    #27205602 - 02/14/21 02:05 PM (2 years, 11 months ago)

I have gymnopilus luteovidris growing rn. I wonder if I could cross them with a cube😎. At sopre level


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OfflineStkn_Hywd_Myco
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Re: Mixed spores on agar [Re: seand04] * 1
    #27213086 - 02/18/21 01:16 PM (2 years, 11 months ago)

:thumbsup:
Reading this thread is getting me excited!!! Got a bunch of new guys I need to grow out and from there we cross!!! ...hopefully...


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InvisibleSunny Skies
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Re: Mixed spores on agar [Re: Stkn_Hywd_Myco]
    #27224102 - 02/24/21 02:02 PM (2 years, 10 months ago)

What if you were to swab two varities with the same swab? Would that work?


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Re: Mixed spores on agar [Re: Sunny Skies] * 1
    #27224288 - 02/24/21 03:48 PM (2 years, 10 months ago)

Quote:

Sunny Skies said:
What if you were to swab two varities with the same swab? Would that work?




That’s how I made RW.


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Offlineseand04
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Re: Mixed spores on agar [Re: Sunny Skies]
    #27224747 - 02/24/21 08:38 PM (2 years, 10 months ago)

Quote:

Sunny Skies said:
What if you were to swab two varities with the same swab? Would that work?



Swab on swab action bomchikawouawhoua....


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Offlinenektar61S
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Re: Mixed spores on agar [Re: Pastywhyte]
    #27224748 - 02/24/21 08:39 PM (2 years, 10 months ago)

Quote:

Pastywhyte said:
Quote:

Sunny Skies said:
What if you were to swab two varities with the same swab? Would that work?




That’s how I made RW.




!!
How many gens until it was stable?


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Offlineseand04
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Re: Mixed spores on agar [Re: Pastywhyte]
    #27224757 - 02/24/21 08:42 PM (2 years, 10 months ago)

Quote:

Pastywhyte said:
Quote:

Sunny Skies said:
What if you were to swab two varities with the same swab? Would that work?




That’s how I made RW.



Bless you for that. I am only growing rw the yields are insane... Thanks pasty... I am gonna try and make my own whytegold. RWGH cross. You the man pasty I started agar because of you're pastyplates... Thanks a million


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InvisibleSunny Skies
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Re: Mixed spores on agar [Re: seand04]
    #27225251 - 02/25/21 05:03 AM (2 years, 10 months ago)

I have some TAT growing atm along with KSSS.I know Wombat created KSAT but would like to try myself..or even cross with PESA or Chitwan. So happy to find sterile swabs here for dirt cheap! @Pasty I had no idea you created RW..right on man! Glad you put our country on the map!


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