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sporecap
Shedding...

Registered: 07/30/18
Posts: 413
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Re: Feet=dipped in agar. How do first time transfers look? [Re: verum subsequentis]
#26925597 - 09/09/20 01:26 AM (3 years, 5 months ago) |
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I have never used food coloring before, so I don't have first-hand experience, but there have been a few posts recently where people have reported the same discoloring caused by the mycelium digesting the food coloring. Otherwise they look fine to me.
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Lenz
Misunderestimated


Registered: 08/17/20
Posts: 692
Loc: Fungistan
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Re: Feet=dipped in agar. How do first time transfers look? [Re: sporecap]
#26925616 - 09/09/20 01:53 AM (3 years, 5 months ago) |
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Quote:
verum subsequentis said: Disclaimer, I'm colorblind and have been dabbling in some wonderful whiskey BUT I'm not sure there's anything wrong with those plates. I'll check again tomorrow to find out.
What colors did you use to make up the agar? If you used any red at all I might know what's happening.
Damn that would be a relief, I used Wilton Neon Gel food coloring teal for the plates. I'll try and take some more pictures later as well to see if I can show the fuzz a little better.
Quote:
sporecap said: I have never used food coloring before, so I don't have first-hand experience, but there have been a few posts recently where people have reported the same discoloring caused by the mycelium digesting the food coloring. Otherwise they look fine to me.
Ahhh, I didn't even think of that. Thought of cobweb sent me down a oneway road to panic lol. Thanks for the input, you very well could be right and I'm overthinking this. Are those posts floating around the first couple pages of Mushroom Cultivation?
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sporecap
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Registered: 07/30/18
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Last seen: 1 year, 10 months
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Re: Feet=dipped in agar. How do first time transfers look? [Re: Lenz]
#26925630 - 09/09/20 02:07 AM (3 years, 5 months ago) |
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redhandmat
Dude


Registered: 05/09/19
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Re: Feet=dipped in agar. How do first time transfers look? [Re: sporecap]
#26925689 - 09/09/20 04:04 AM (3 years, 5 months ago) |
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They look good OP! if you slightly decrease the amount of nutes in your next plates you will likely get more rhizo growth (just a tip). IMO you have done real good!
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verum subsequentis
seeker of truth



Registered: 03/22/16
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Re: Feet=dipped in agar. How do first time transfers look? [Re: redhandmat]
#26927231 - 09/09/20 09:46 PM (3 years, 5 months ago) |
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I didn't read those threads but yes, myc indeed eats food coloring. But it doesn't eat red (at least some brands) because it's made from a bug with anti fungal properties. Ask me how i know.
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Lenz
Misunderestimated


Registered: 08/17/20
Posts: 692
Loc: Fungistan
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Re: Feet=dipped in agar. How do first time transfers look? [Re: verum subsequentis]
#26927346 - 09/09/20 11:35 PM (3 years, 5 months ago) |
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Warning, gnarly plates.
So here are some other transfers that I did a day after the other ones I've posted. The strange growth is a bit more visible here, and it is very similar to the growth on the other plates. Would love to think it's normal myc but looking at these again it seems pretty evident that something is up. Some are more obviously contam'd like B2,B4,B10,B14,and B15, just thought I'd throw those in for comparison.
I made 4 transfers each last night from the #10 and #6 plates from the previous post, we'll see how they go.
Poured 40 more plates as well, tried a new bottle since my last one exploded on me lol. I'm using 1L glass Heinz vinegar bottles. Worked pretty well except for a bit of drippage, but otherwise I'm liking them. I modded the lids by drilling a hole in the center and sticking an SFD underneath. I'm going to try and let these plates sit for about week without using them just to make sure I'm PCing and pouring correctly. Need to start eliminating potential vectors to be safe, it would suck to have contam be a recurring issue going forward.
Anyways, here's the pics of a diff batch of transfers:
#B1:

#B2:

#B3:

#B4:

#B5:

#B6:

#B7:

#B8:
 
#B9:

#B10:

#B11:

#B12:

#B13:

#B14:

#B15:

#B16:
Edited by Lenz (09/09/20 11:39 PM)
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Lenz
Misunderestimated


Registered: 08/17/20
Posts: 692
Loc: Fungistan
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Re: Feet=dipped in agar. How do first time transfers look? [Re: verum subsequentis]
#26927348 - 09/09/20 11:38 PM (3 years, 5 months ago) |
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Quote:
verum subsequentis said: I didn't read those threads but yes, myc indeed eats food coloring. But it doesn't eat red (at least some brands) because it's made from a bug with anti fungal properties. Ask me how i know.
Interesting, how do you know? Can it be shared with non TC's?
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WunFunGai
Wanderer

Registered: 05/03/16
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Re: Feet=dipped in agar. How do first time transfers look? [Re: Lenz]
#26927396 - 09/10/20 12:28 AM (3 years, 5 months ago) |
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Hiya, thanks for sharing those!
It's really helpful to see others plates and how they progress.
I feel like #5...More input!!! LOL
WFG
-------------------- The Official TNF Reccomended Teks & Methods Bod's Simplified Cultivation Methods <-- Awesome!!! D3monics Perfect Transfers and Agar Tek No matter where you go, there you are... - Buckaroo Banzai You gotta be here, if you're not all there...
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sporecap
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Registered: 07/30/18
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Last seen: 1 year, 10 months
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Re: Feet=dipped in agar. How do first time transfers look? [Re: Lenz]
#26927422 - 09/10/20 12:58 AM (3 years, 5 months ago) |
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Quote:
Lenz said:
Quote:
verum subsequentis said: I didn't read those threads but yes, myc indeed eats food coloring. But it doesn't eat red (at least some brands) because it's made from a bug with anti fungal properties. Ask me how i know.
Interesting, how do you know? Can it be shared with non TC's? 
I think verum is referring to this  These Crazy Pictures Show How Companies Use Bugs To Make Red Dye For Food And Cosmetics
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redhandmat
Dude


Registered: 05/09/19
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Re: Feet=dipped in agar. How do first time transfers look? [Re: sporecap]
#26927673 - 09/10/20 06:12 AM (3 years, 5 months ago) |
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Some of those plates are contaminated OP. You should transfer from the best looking ones and then transfer again when you see a tiny bit grwth.
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verum subsequentis
seeker of truth



Registered: 03/22/16
Posts: 8,732
Last seen: 1 year, 8 months
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Re: Feet=dipped in agar. How do first time transfers look? [Re: redhandmat]
#26928191 - 09/10/20 12:22 PM (3 years, 5 months ago) |
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B4 is obiously fucked but could be saved with proper sab tek. B14 is almost certainly carrying a nasty and i'd probably just toss her. The rest look anywhere from great to probably fine. I'd have to look closely in person to be absolutely certain but i think you've got some workable cultures.
I know about the food coloring issue because i'm a super nerd and i've grown cultures in thousands and thousands of plates with varying amounts of various colors and watched how they acted. I once did a a batch of green plates and a batch of red plates in an attempt to keep track of something that i can't remember. I think i was monitoring the difference in growth taken from rhizo and tom parts of the same semi isolated cultures. The pics are somewhere in the stupidly long "how not to isolate" thread in my sig. All the cultures in thread red plates performed so shittily that i was rather certain it wasn't coincidence or culture health.
Other times i noticed that any plate of made up of red and another color would slowly turn red as the culture grew.
Other times i just noticed that any color other than red would slowly disappear and leave the plate eventually almost the natural color of agar. This lead me to believe that myc did indeed eat the food coloring but for some reason wouldn't eat red. So i did a test in which i made some plates with a tiny bit of red and some with a shitload. The cultures on slightly red agar did fine but the cultures swimming in deep red fucking died.
This led me to do a little reading and i quickly found an article detailing the process by which red food coloring is harvested and how it is poisonous to myc.
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WunFunGai
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Re: Feet=dipped in agar. How do first time transfers look? [Re: verum subsequentis]
#26928552 - 09/10/20 03:49 PM (3 years, 5 months ago) |
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Quote:
sporecap said:
Quote:
Lenz said:
Quote:
verum subsequentis said: I didn't read those threads but yes, myc indeed eats food coloring. But it doesn't eat red (at least some brands) because it's made from a bug with anti fungal properties. Ask me how i know.
Interesting, how do you know? Can it be shared with non TC's? 
I think verum is referring to this  These Crazy Pictures Show How Companies Use Bugs To Make Red Dye For Food And Cosmetics
Quote:
verum subsequentis said: B4 is obiously fucked but could be saved with proper sab tek. B14 is almost certainly carrying a nasty and i'd probably just toss her. The rest look anywhere from great to probably fine. I'd have to look closely in person to be absolutely certain but i think you've got some workable cultures.
I know about the food coloring issue because i'm a super nerd and i've grown cultures in thousands and thousands of plates with varying amounts of various colors and watched how they acted. I once did a a batch of green plates and a batch of red plates in an attempt to keep track of something that i can't remember. I think i was monitoring the difference in growth taken from rhizo and tom parts of the same semi isolated cultures. The pics are somewhere in the stupidly long "how not to isolate" thread in my sig. All the cultures in thread red plates performed so shittily that i was rather certain it wasn't coincidence or culture health.
Other times i noticed that any plate of made up of red and another color would slowly turn red as the culture grew.
Other times i just noticed that any color other than red would slowly disappear and leave the plate eventually almost the natural color of agar. This lead me to believe that myc did indeed eat the food coloring but for some reason wouldn't eat red. So i did a test in which i made some plates with a tiny bit of red and some with a shitload. The cultures on slightly red agar did fine but the cultures swimming in deep red fucking died.
This led me to do a little reading and i quickly found an article detailing the process by which red food coloring is harvested and how it is poisonous to myc.
Hey, thanks for sharing the links an anecdotal observations! Super helpful and nerdy too 
WFG
-------------------- The Official TNF Reccomended Teks & Methods Bod's Simplified Cultivation Methods <-- Awesome!!! D3monics Perfect Transfers and Agar Tek No matter where you go, there you are... - Buckaroo Banzai You gotta be here, if you're not all there...
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verum subsequentis
seeker of truth



Registered: 03/22/16
Posts: 8,732
Last seen: 1 year, 8 months
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Re: Feet=dipped in agar. How do first time transfers look? [Re: WunFunGai]
#26928864 - 09/10/20 07:14 PM (3 years, 5 months ago) |
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