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Offlinepitriot
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What is this on the agar?
    #26928563 - 09/10/20 03:54 PM (3 years, 4 months ago)

These little (black?) dots/pins just happened last few days. What are they?





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OfflineOne of Us
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Re: What is this on the agar? [Re: pitriot]
    #26928587 - 09/10/20 04:12 PM (3 years, 4 months ago)

Knots/pins


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OfflineA.k.aM
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Re: What is this on the agar? [Re: pitriot]
    #26928588 - 09/10/20 04:12 PM (3 years, 4 months ago)

Just knots. The plate fully colonized and went into fruiting. It’s pretty cool to see how the knots pop up along the rhizo veins.

Pretty soon you’ll have some pins go clone if you want.


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Offlinepitriot
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Re: What is this on the agar? [Re: A.k.a]
    #26928621 - 09/10/20 04:29 PM (3 years, 4 months ago)

Quote:

A.k.a said:
Just knots. The plate fully colonized and went into fruiting. It’s pretty cool to see how the knots pop up along the rhizo veins.

Pretty soon you’ll have some pins go clone if you want.




Do I need pins to clone, wouldn't it be the same to just transfer the agar?


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Offlinehazyhorse
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Re: What is this on the agar? [Re: pitriot]
    #26928689 - 09/10/20 05:25 PM (3 years, 4 months ago)

i’m not sure if transferring the knot would end up with a clone, but transferring a pin is more surefire. if you’re talking about just transferring some of the mycelium in general, instead of a knot/pin, i don’t think it would clone


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Offlinepitriot
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Re: What is this on the agar? [Re: hazyhorse]
    #26929216 - 09/11/20 12:04 AM (3 years, 4 months ago)

What is the difference between clone and transfer and what is the advantage of cloning?


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Offlinehazyhorse
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Re: What is this on the agar? [Re: pitriot]
    #26929228 - 09/11/20 12:14 AM (3 years, 4 months ago)

when you do multispore to agar, you have a bunch of genetics that may not act very uniformly across the mushrooms, but by cloning (taking some tissue out of the middle of a mushroom & putting it to agar) you can get a culture that is less varied in genetics & will potentially yield better & more uniformly & will have more consistent potency across the flush.

taking a normal transfer on agar from an original multispore plate is just grabbing a bunch of different genetics i believe, tho i’m sure transferring narrows them down a bit & you can isolate cultures on agar if you’re good which is kinda similar to cloning but more intense


cloning is just a lot easier & more worth it to do generally


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InvisibleGoatrider
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Re: What is this on the agar? [Re: pitriot]
    #26929230 - 09/11/20 12:16 AM (3 years, 4 months ago)

A clone would be good to take cause it grew under "poor" conditions.
Imagine that going on grains, that's a great requirement for good pinsets.


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