|
pitriot
Stranger


Registered: 06/16/19
Posts: 455
Last seen: 3 months, 4 days
|
What is this on the agar?
#26928563 - 09/10/20 03:54 PM (3 years, 4 months ago) |
|
|
These little (black?) dots/pins just happened last few days. What are they?

|
One of Us
Stranger



Registered: 03/12/12
Posts: 1,140
Last seen: 6 days, 16 hours
|
Re: What is this on the agar? [Re: pitriot]
#26928587 - 09/10/20 04:12 PM (3 years, 4 months ago) |
|
|
Knots/pins
|
A.k.a
Stranger



Registered: 10/27/19
Posts: 16,782
Loc: Gaming the system
Last seen: 3 hours, 21 minutes
|
Re: What is this on the agar? [Re: pitriot]
#26928588 - 09/10/20 04:12 PM (3 years, 4 months ago) |
|
|
Just knots. The plate fully colonized and went into fruiting. It’s pretty cool to see how the knots pop up along the rhizo veins.
Pretty soon you’ll have some pins go clone if you want.
--------------------
LAGM2020     
|
pitriot
Stranger


Registered: 06/16/19
Posts: 455
Last seen: 3 months, 4 days
|
Re: What is this on the agar? [Re: A.k.a]
#26928621 - 09/10/20 04:29 PM (3 years, 4 months ago) |
|
|
Quote:
A.k.a said: Just knots. The plate fully colonized and went into fruiting. It’s pretty cool to see how the knots pop up along the rhizo veins.
Pretty soon you’ll have some pins go clone if you want.
Do I need pins to clone, wouldn't it be the same to just transfer the agar?
|
hazyhorse
scoobin



Registered: 03/19/19
Posts: 3,820
Last seen: 16 hours, 41 minutes
|
Re: What is this on the agar? [Re: pitriot]
#26928689 - 09/10/20 05:25 PM (3 years, 4 months ago) |
|
|
i’m not sure if transferring the knot would end up with a clone, but transferring a pin is more surefire. if you’re talking about just transferring some of the mycelium in general, instead of a knot/pin, i don’t think it would clone
-------------------- you're not the first to set foot here, just another =================================== i love glass petris & you can too!! posts i constantly refer back to new to mushroom cultivation?? read this!! ===================================
  🅃 🄴 🄰 🄼 🄲 🄻 🄸 🄽 🄶 🅆 🅁 🄰 🄿
|
pitriot
Stranger


Registered: 06/16/19
Posts: 455
Last seen: 3 months, 4 days
|
Re: What is this on the agar? [Re: hazyhorse]
#26929216 - 09/11/20 12:04 AM (3 years, 4 months ago) |
|
|
What is the difference between clone and transfer and what is the advantage of cloning?
|
hazyhorse
scoobin



Registered: 03/19/19
Posts: 3,820
Last seen: 16 hours, 41 minutes
|
Re: What is this on the agar? [Re: pitriot]
#26929228 - 09/11/20 12:14 AM (3 years, 4 months ago) |
|
|
when you do multispore to agar, you have a bunch of genetics that may not act very uniformly across the mushrooms, but by cloning (taking some tissue out of the middle of a mushroom & putting it to agar) you can get a culture that is less varied in genetics & will potentially yield better & more uniformly & will have more consistent potency across the flush.
taking a normal transfer on agar from an original multispore plate is just grabbing a bunch of different genetics i believe, tho i’m sure transferring narrows them down a bit & you can isolate cultures on agar if you’re good which is kinda similar to cloning but more intense
cloning is just a lot easier & more worth it to do generally
|
Goatrider
Rhythm Guitarist



Registered: 04/08/20
Posts: 4,396
Loc: Germany
|
Re: What is this on the agar? [Re: pitriot]
#26929230 - 09/11/20 12:16 AM (3 years, 4 months ago) |
|
|
A clone would be good to take cause it grew under "poor" conditions. Imagine that going on grains, that's a great requirement for good pinsets.
|
|
|
You cannot start new topics / You cannot reply to topics HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a 133 topic views. 21 members, 164 guests and 51 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ] |
|