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poncho anatomy
Stranger
Registered: 06/07/20
Posts: 10
Last seen: 3 years, 5 months
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New to agar! Help me identify this contam
#26914323 - 09/02/20 06:29 PM (3 years, 5 months ago) |
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Background information I am new to agar, and I have one successful PF Tek grow to my name.
The recipe I used was 9g agar-agar, 6g brf, 500ml water. I ground up the brf myself in a coffee grinder, so it was fairly coarse but I do appear to get growth on my plates.
Exhibit A - Source (t4) and Destination (t5) Originally from spores
 
this is currently the most successful-looking plate I have. The pic on the left is the source of the transfer, and the pic on the right is the destination. I'm seeing rhizomorphic growth. When I look carefully, I notice little concentric rings (like the rings on a tree stump) that deliniate the myc. I'm guessing it has to do with temperature fluctuations (for example, my A/C went out one day while this myc was colonizing). Is that guess accurate? Or is there a better explanation?
Exhibit B - destination (t3) Originally from spores

I only have the destination for Exhibit B. Is this healthy tomentose myc? Is it some kind of contamination? Is it bacteria making it look fuzzy? I don't have anything to compare it to, because this is my first time doing agar.
Exhibit C - Source (t3) and Destination (t4) Originally from cloned fruit
 
Same as before: source on the left, and destination on the right.
Questions about the source: Why is it so fuzzy and tomentose in the middle, and only rhizomorphic on the edges? Is that due to some contamination, or could that be considered normal?
Questions about the destination: You can see that this came from a rhizomorphic sector -- why is so little of it rhizomorphic after the transfer?
Exhibit D - Source (t3) and Destination (t4) Originally from cloned fruit
 
Similar questions to the above. Cloned from a rhizomorphic sector, but has very little rhizomorphic growth after the transfer, and even that is fuzzy.
Edited by poncho anatomy (09/09/20 12:04 AM)
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BBH224



Registered: 07/28/13
Posts: 86
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Re: Need some eyes on my agar -- pics -- specific questions [Re: poncho anatomy]
#26914482 - 09/02/20 08:03 PM (3 years, 5 months ago) |
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I just started agar and am trying to consume all questions and replies from experienced cultivators. Great question and write up for this. Curious to see what they say. Sorry all I could contribute is a bump.
-------------------- I love boobs because you can't motorboat a personality
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poncho anatomy
Stranger
Registered: 06/07/20
Posts: 10
Last seen: 3 years, 5 months
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Re: Need some eyes on my agar -- pics -- specific questions [Re: BBH224]
#26915249 - 09/03/20 09:32 AM (3 years, 5 months ago) |
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Quote:
I just started agar and am trying to consume all questions and replies from experienced cultivators. Great question and write up for this. Curious to see what they say. Sorry all I could contribute is a bump.
Thanks for the kind words and the bump Hopefully some experienced hands will be able to tell me what I'm doing wrong. Hope it benefits your efforts too
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ruK
Stranger


Registered: 05/27/20
Posts: 83
Last seen: 2 years, 4 months
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Re: New to agar! Critique my pics like a pro [Re: poncho anatomy]
#26915402 - 09/03/20 11:14 AM (3 years, 5 months ago) |
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I'm interested to see what people say about the translucency in the center of the dishes. O_o; Is it diving down to eat or is that indicative of something else?
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poncho anatomy
Stranger
Registered: 06/07/20
Posts: 10
Last seen: 3 years, 5 months
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Re: New to agar! Critique my pics like a pro [Re: ruK]
#26925533 - 09/09/20 12:01 AM (3 years, 5 months ago) |
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I transferred from spores/t5 to this plate here:
 The mycelium is growing, but I noticed a very fast-growing and faint ring around the mycelial growth. The ring grew to cover the whole surface of the agar within 3 days. You'll notice some chunks missing -- I decided to isolate the ring to see what it grows into. The following images show the growth of whatever that ring was.
  
Can anyone tell me what this is? You can see the edges of it in these images here. I'm guessing it's some kind of bacteria. this only developed on plates that originally came from spores; the clones didn't have any kind of ring that spread like that.
If this is bacteria, I might as well start over with some fresh spores. Thanks in advance for the help! Hope the others responding to this thread can get something out of my experiment.
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sporecap
Shedding...

Registered: 07/30/18
Posts: 413
Loc: Europe
Last seen: 1 year, 10 months
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Re: New to agar! Critique my pics like a pro [Re: poncho anatomy]
#26925599 - 09/09/20 01:31 AM (3 years, 5 months ago) |
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Being this fast and whispy, this for sure is mold. Probably it's easier to start again from spores than trying to clean it up.
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Lenz
Misunderestimated


Registered: 08/17/20
Posts: 692
Loc: Fungistan
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Re: New to agar! Critique my pics like a pro [Re: sporecap]
#26925624 - 09/09/20 01:58 AM (3 years, 5 months ago) |
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Hey, relatively new here as well. I've been seeing some similar wispy halo's on my plates as well, albeit not as fast growing as yours, along with a similar almost orangeish centered growth like your Exhibit A T4 plate. Have a thread up about my plates if you want to compare. Someone else said the orangeish color could just be from the myc eating the food coloring but I'm not sure. Good luck!
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poncho anatomy
Stranger
Registered: 06/07/20
Posts: 10
Last seen: 3 years, 5 months
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Re: New to agar! Critique my pics like a pro [Re: Lenz]
#26926022 - 09/09/20 09:56 AM (3 years, 5 months ago) |
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Quote:
Being this fast and whispy, this for sure is mold. Probably it's easier to start again from spores than trying to clean it up.
Thank you for this -- you are definitely right. It didn't look like mold at first, it just looked kinda wet. as it has grown out, it's definitely more obviously mold, and it's starting to get fuzzy.
Thanks for taking the time to help out!
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