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Wall.E
Bacteria's Bitch



Registered: 06/05/20
Posts: 2,860
Loc: Fungal Void
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Getting rid of a tree stump with pleurotus citirinopileatus?
#26884900 - 08/17/20 01:23 PM (3 years, 5 months ago) |
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I know this may be a stupid question regarding "training" but I didn't want to ask it in the mush cult forum.
If I were to try and train a pleurotus citirinopileatus isolate, would I still put LME in my agar if it's already used to it? Or do I make my next agar solely the sawdust, agar and water?
My plan was to use LME and some saw dust and increase the sawdust amounts and reduce the LME amounts with each transfer, but I've never done this and don't really know the terminology to search for.
Anyways, thanks for any attention you're willing to give me.
-------------------- Life’s shit, but I’m loving it
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Forrester
aspiring sociopath


Registered: 02/05/13
Posts: 9,351
Loc: Northeast USA
Last seen: 1 month, 12 days
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Re: Getting rid of a tree stump with pleurotus citirinopileatus? [Re: Wall.E]
#26918640 - 09/05/20 04:59 AM (3 years, 5 months ago) |
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What are you trying to do exactly? Pleurotus already grows on wood
-------------------- Repugnant is a creature who would squander the ability to lift an eye to heaven, conscious of his fleeting time here. ------------------- Have some medicinal mushrooms and want to get the most out of them? Try this double extraction method.
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Wall.E
Bacteria's Bitch



Registered: 06/05/20
Posts: 2,860
Loc: Fungal Void
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Re: Getting rid of a tree stump with pleurotus citirinopileatus? [Re: Forrester]
#26923244 - 09/07/20 02:54 PM (3 years, 5 months ago) |
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Well I know that and that's why I was going this way. The tree was planted by a few owners before me, so I don't remember what kind of tree it was or anything. The pleurotus was from a clone that I managed to grab from an experiment I did. I was growing it out for a few transfers before this idea came to me.
So my rationale was that this mushroom came from a coffee/straw based substrate and has been on LME agar, so I didn't want to just blend it up and inoculate the stump before at least making sure it would eat the tree.
I thought this thread died, sorry. I've been working in some sawdust into agar mixtures and doing transfers. I'm almost up to 50/50. At some point that's basically my plan, just blend it up and pour it down the middle of the stump and seal it off. It's not like I was going to do anything else with the stump, so I figured I might as well try something.
-------------------- Life’s shit, but I’m loving it
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Forrester
aspiring sociopath


Registered: 02/05/13
Posts: 9,351
Loc: Northeast USA
Last seen: 1 month, 12 days
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Re: Getting rid of a tree stump with pleurotus citirinopileatus? [Re: Wall.E]
#26923303 - 09/07/20 03:54 PM (3 years, 5 months ago) |
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Yeah it'll take care of the stump no problem, you just don't need to train it to like wood, it's a completely unnecessary step. The species has been decaying wood for probably thousands of years or more, it knows what to do 
If anything you're probably just making it a little tired running it unnecessarily, though I'm sure it's fine.
-------------------- Repugnant is a creature who would squander the ability to lift an eye to heaven, conscious of his fleeting time here. ------------------- Have some medicinal mushrooms and want to get the most out of them? Try this double extraction method.
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Wall.E
Bacteria's Bitch



Registered: 06/05/20
Posts: 2,860
Loc: Fungal Void
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Re: Getting rid of a tree stump with pleurotus citirinopileatus? [Re: Forrester]
#26923743 - 09/07/20 08:14 PM (3 years, 5 months ago) |
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Okay, totally new to this hobby and thought I needed to do that for some reason. Once I get growth on these plates I'll blend em up and inoculate. Thank you for the help
-------------------- Life’s shit, but I’m loving it
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