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OfflineFarmerEric
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Time to shake?
    #26922574 - 09/07/20 07:54 AM (3 years, 5 months ago)

Long time, first time!

I made a few jars and inoculated 6 days ago. Did both a coffee WBS and regular WBS.

Is it time to break up these jars yet? Pictures. Are taken from opposite sides of the jars. Thanks!

Planning to put these in CVG shoeboxes.



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Re: Time to shake? [Re: FarmerEric]
    #26922597 - 09/07/20 08:15 AM (3 years, 5 months ago)

Quote:

FarmerEric said:
Long time, first time!

I made a few jars and inoculated 6 days ago. Did both a coffee WBS and regular WBS.

Is it time to break up these jars yet? Pictures. Are taken from opposite sides of the jars. Thanks!

Planning to put these in CVG shoeboxes.





Inoculated with what? I hate to say it, but those jars look very suspect for just 6 days especially if you're about to tell me a spore syringe :facepalm:

Oh and the coffee too....that makes this even more suspect. I think you got mold man.


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Re: Time to shake? [Re: Tormato]
    #26922603 - 09/07/20 08:18 AM (3 years, 5 months ago)

Yeah my thoughts exactly.

That thin wispy stuff is not good unless you’re doing lions mane.


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OfflineFarmerEric
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Re: Time to shake? [Re: Tormato]
    #26922606 - 09/07/20 08:19 AM (3 years, 5 months ago)

F. Yes syringe. Any way to tell? One is coffee without sunflower, the other is normal wbs.

B+ strain


Edited by FarmerEric (09/07/20 08:20 AM)


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OfflineFarmerEric
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Re: Time to shake? [Re: FarmerEric]
    #26922612 - 09/07/20 08:22 AM (3 years, 5 months ago)



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Re: Time to shake? [Re: FarmerEric]
    #26922619 - 09/07/20 08:25 AM (3 years, 5 months ago)

They should look more like this


The really thin hairy looking growth on yours looks like some type of white/grey mold. It’s hard to tell sometimes especially when you’re just starting.

I didn’t even see that it’s been six days from spores. You should have at best a few quarter size spots of myc at this point.


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Edited by A.k.a (09/07/20 08:27 AM)


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Re: Time to shake? [Re: FarmerEric]
    #26922620 - 09/07/20 08:25 AM (3 years, 5 months ago)

Quote:

FarmerEric said:
F. Yes syringe. Any way to tell? One is coffee without sunflower, the other is normal wbs.

B+ strain



For me a key element was the time frame....6 days is not a lot of time for that much growth, especial from spores.

The other was the appearance of the myc. Cube myc in particular is a bit more thick, whiter and just has a healthier appearance. Thin and wispy usually isn't a good thing with cubes.



Quote:

FarmerEric said:




Oh yeah....that's definitely mold bro...toss!


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OfflineFarmerEric
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Re: Time to shake? [Re: A.k.a]
    #26922630 - 09/07/20 08:32 AM (3 years, 5 months ago)

OK, what's your take on these jars (diff strain also syringe, inoc same time)



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OfflineFarmerEric
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Re: Time to shake? [Re: Tormato]
    #26922636 - 09/07/20 08:35 AM (3 years, 5 months ago)

Ah Frick. Likely contaminated syringe? This wbs was washed, soaked, pc'd 90min @ 15psi, inoculated next day in SAB.

I have 5 agar plates growing too. The syringes were older. Plan is to let the 5 plates grow, isolate and transfer to a new plate for each of the 5 and then to grain masters.


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Re: Time to shake? [Re: FarmerEric]
    #26922637 - 09/07/20 08:35 AM (3 years, 5 months ago)

Much better. Keep an eye on it though, sometimes mold won’t show up until like 10 days in or more.

Some spots still look sketchy but that’s at least actual myc in there.


Actually after looking closer I think the second pic has mold underneath the top myc sprout. The whole middle area where there’s growth looks like similar mold to the first jars in the op.

How do your plates look? Idk where the syringes came from but starting to seem likely they’re responsible. If the plates grow the same shit I’d try to get a refund.


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Edited by A.k.a (09/07/20 08:40 AM)


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OfflineFarmerEric
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Re: Time to shake? [Re: A.k.a]
    #26922642 - 09/07/20 08:41 AM (3 years, 5 months ago)

Quote:

A.k.a said:
Much better. Keep an eye on it though, sometimes mold won’t show up until like 10 days in or more.

Some spots still look sketchy but that’s at least actual myc in there.




Total noob, where did I likely go wrong? Too much spore solution changing the moisture in the jar?


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Re: Time to shake? [Re: FarmerEric]
    #26922650 - 09/07/20 08:46 AM (3 years, 5 months ago)

Quote:

FarmerEric said:
Quote:

A.k.a said:
Much better. Keep an eye on it though, sometimes mold won’t show up until like 10 days in or more.

Some spots still look sketchy but that’s at least actual myc in there.




Total noob, where did I likely go wrong? Too much spore solution changing the moisture in the jar?



Bad Syringe or sterile technique


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Re: Time to shake? [Re: FarmerEric]
    #26922654 - 09/07/20 08:49 AM (3 years, 5 months ago)

No spores are usually carrying contamination mixed in with them. Certain vendors use machines that remove most of it but I only know of one place that does that for sure.

I’d guess the syringes are loaded with spores from that mold. Moisture won’t make contams materialize in the jar but it will make it easier for them to grow if they’re already in there.


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OfflineFarmerEric
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Re: Time to shake? [Re: A.k.a]
    #26922656 - 09/07/20 08:49 AM (3 years, 5 months ago)

Quote:

A.k.a said:
Much better. Keep an eye on it though, sometimes mold won’t show up until like 10 days in or more.

Some spots still look sketchy but that’s at least actual myc in there.


Actually after looking closer I think the second pic has mold underneath the top myc sprout. The whole middle area where there’s growth looks like similar mold to the first jars in the op.

How do your plates look? Idk where the syringes came from but starting to seem likely they’re responsible. If the plates grow the same shit I’d try to get a refund.





Syringes are from a vendor from a different site. Plates were done via spore prints & swabs.

Here's the plates in order: Maz, wild gt, & mammoth gt. First time doing any of this so yes, lots of spore on the plates. Growth originated from a spore clump on the wild gt plate.




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OfflineFarmerEric
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Re: Time to shake? [Re: A.k.a]
    #26922658 - 09/07/20 08:51 AM (3 years, 5 months ago)

Quote:

A.k.a said:
No spores are usually carrying contamination mixed in with them. Certain vendors use machines that remove most of it but I only know of one place that does that for sure.

I’d guess the syringes are loaded with spores from that mold. Moisture won’t make contams materialize in the jar but it will make it easier for them to grow if they’re already in there.




My plan is for agar and g2g I only tried these syringes bc I had them left over and thought why not. They worked in April but I messed up the shoebox and it got contam (opened way too often).


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Re: Time to shake? [Re: FarmerEric]
    #26922681 - 09/07/20 09:11 AM (3 years, 5 months ago)

Quote:

FarmerEric said:
got contam (opened way too often).



That's not why you got contams...it's was your spawn and from what I'm gathering it is indeed the syringe or your sterile practice or both. Try the syringe again on agar and work for a clean culture.


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OfflineFarmerEric
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Re: Time to shake? [Re: Tormato]
    #26922692 - 09/07/20 09:16 AM (3 years, 5 months ago)

Quote:

Tormato said:
Quote:

FarmerEric said:
got contam (opened way too often).



That's not why you got contams...it's was your spawn and from what I'm gathering it is indeed the syringe or your sterile practice or both. Try the syringe again on agar and work for a clean culture.




Makes sense the syringe would be to blame. I had a 3lb bag of oats that went green mold and I thought it was the grain. The bag was a different syringe than the clearly bad jars but both syringes from the same seller.

To use a syringe onnagar I'd aim for just a couple drops of water in the middle correct?

Thank you all for the help and tough love. It sucks to hear these are lost but I'm glad to learn from this to avoid it in the future!

Side question: how long do grain jars remain "good" on average after pc, a week?


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Re: Time to shake? [Re: FarmerEric]
    #26922702 - 09/07/20 09:26 AM (3 years, 5 months ago)

With the syringe try to get one drop on the plate if you can, as little as possible.

Prepped jars will be fine after a week, and even longer depending on how much moisture they’re losing.


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Re: Time to shake? [Re: FarmerEric]
    #26922704 - 09/07/20 09:28 AM (3 years, 5 months ago)

One drop is plenty.

If I don't use my sterilized grains within a week I start over...some have gone longer, but I worry more about the hydration of the grains versus contams when letting them sit unused for awhile.

What A.k.a. said too.


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OfflineFarmerEric
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Re: Time to shake? [Re: FarmerEric]
    #26922715 - 09/07/20 09:33 AM (3 years, 5 months ago)

Quote:

FarmerEric said:
Quote:

A.k.a said:
Much better. Keep an eye on it though, sometimes mold won’t show up until like 10 days in or more.

Some spots still look sketchy but that’s at least actual myc in there.


Actually after looking closer I think the second pic has mold underneath the top myc sprout. The whole middle area where there’s growth looks like similar mold to the first jars in the op.

How do your plates look? Idk where the syringes came from but starting to seem likely they’re responsible. If the plates grow the same shit I’d try to get a refund.





Syringes are from a vendor from a different site. Plates were done via spore prints & swabs.

Here's the plates in order: Maz, wild gt, & mammoth gt. First time doing any of this so yes, lots of spore on the plates. Growth originated from a spore clump on the wild gt plate.








Any wisdom for my plates? They're supposed to be wrapped in parafilm during incubation right?

And since this one is near the edge should I isolate and move to a new plate?




Edited by FarmerEric (09/07/20 09:47 AM)


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