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OfflineFarmerEric
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Time to shake?
    #26922574 - 09/07/20 07:54 AM (3 years, 4 months ago)

Long time, first time!

I made a few jars and inoculated 6 days ago. Did both a coffee WBS and regular WBS.

Is it time to break up these jars yet? Pictures. Are taken from opposite sides of the jars. Thanks!

Planning to put these in CVG shoeboxes.



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Re: Time to shake? [Re: FarmerEric]
    #26922597 - 09/07/20 08:15 AM (3 years, 4 months ago)

Quote:

FarmerEric said:
Long time, first time!

I made a few jars and inoculated 6 days ago. Did both a coffee WBS and regular WBS.

Is it time to break up these jars yet? Pictures. Are taken from opposite sides of the jars. Thanks!

Planning to put these in CVG shoeboxes.





Inoculated with what? I hate to say it, but those jars look very suspect for just 6 days especially if you're about to tell me a spore syringe :facepalm:

Oh and the coffee too....that makes this even more suspect. I think you got mold man.


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Re: Time to shake? [Re: Tormato]
    #26922603 - 09/07/20 08:18 AM (3 years, 4 months ago)

Yeah my thoughts exactly.

That thin wispy stuff is not good unless you’re doing lions mane.


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OfflineFarmerEric
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Re: Time to shake? [Re: Tormato]
    #26922606 - 09/07/20 08:19 AM (3 years, 4 months ago)

F. Yes syringe. Any way to tell? One is coffee without sunflower, the other is normal wbs.

B+ strain


Edited by FarmerEric (09/07/20 08:20 AM)


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OfflineFarmerEric
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Re: Time to shake? [Re: FarmerEric]
    #26922612 - 09/07/20 08:22 AM (3 years, 4 months ago)



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Re: Time to shake? [Re: FarmerEric]
    #26922619 - 09/07/20 08:25 AM (3 years, 4 months ago)

They should look more like this


The really thin hairy looking growth on yours looks like some type of white/grey mold. It’s hard to tell sometimes especially when you’re just starting.

I didn’t even see that it’s been six days from spores. You should have at best a few quarter size spots of myc at this point.


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Edited by A.k.a (09/07/20 08:27 AM)


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Re: Time to shake? [Re: FarmerEric]
    #26922620 - 09/07/20 08:25 AM (3 years, 4 months ago)

Quote:

FarmerEric said:
F. Yes syringe. Any way to tell? One is coffee without sunflower, the other is normal wbs.

B+ strain



For me a key element was the time frame....6 days is not a lot of time for that much growth, especial from spores.

The other was the appearance of the myc. Cube myc in particular is a bit more thick, whiter and just has a healthier appearance. Thin and wispy usually isn't a good thing with cubes.



Quote:

FarmerEric said:




Oh yeah....that's definitely mold bro...toss!


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OfflineFarmerEric
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Re: Time to shake? [Re: A.k.a]
    #26922630 - 09/07/20 08:32 AM (3 years, 4 months ago)

OK, what's your take on these jars (diff strain also syringe, inoc same time)



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OfflineFarmerEric
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Re: Time to shake? [Re: Tormato]
    #26922636 - 09/07/20 08:35 AM (3 years, 4 months ago)

Ah Frick. Likely contaminated syringe? This wbs was washed, soaked, pc'd 90min @ 15psi, inoculated next day in SAB.

I have 5 agar plates growing too. The syringes were older. Plan is to let the 5 plates grow, isolate and transfer to a new plate for each of the 5 and then to grain masters.


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Re: Time to shake? [Re: FarmerEric]
    #26922637 - 09/07/20 08:35 AM (3 years, 4 months ago)

Much better. Keep an eye on it though, sometimes mold won’t show up until like 10 days in or more.

Some spots still look sketchy but that’s at least actual myc in there.


Actually after looking closer I think the second pic has mold underneath the top myc sprout. The whole middle area where there’s growth looks like similar mold to the first jars in the op.

How do your plates look? Idk where the syringes came from but starting to seem likely they’re responsible. If the plates grow the same shit I’d try to get a refund.


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Edited by A.k.a (09/07/20 08:40 AM)


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OfflineFarmerEric
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Re: Time to shake? [Re: A.k.a]
    #26922642 - 09/07/20 08:41 AM (3 years, 4 months ago)

Quote:

A.k.a said:
Much better. Keep an eye on it though, sometimes mold won’t show up until like 10 days in or more.

Some spots still look sketchy but that’s at least actual myc in there.




Total noob, where did I likely go wrong? Too much spore solution changing the moisture in the jar?


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Re: Time to shake? [Re: FarmerEric]
    #26922650 - 09/07/20 08:46 AM (3 years, 4 months ago)

Quote:

FarmerEric said:
Quote:

A.k.a said:
Much better. Keep an eye on it though, sometimes mold won’t show up until like 10 days in or more.

Some spots still look sketchy but that’s at least actual myc in there.




Total noob, where did I likely go wrong? Too much spore solution changing the moisture in the jar?



Bad Syringe or sterile technique


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Re: Time to shake? [Re: FarmerEric]
    #26922654 - 09/07/20 08:49 AM (3 years, 4 months ago)

No spores are usually carrying contamination mixed in with them. Certain vendors use machines that remove most of it but I only know of one place that does that for sure.

I’d guess the syringes are loaded with spores from that mold. Moisture won’t make contams materialize in the jar but it will make it easier for them to grow if they’re already in there.


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OfflineFarmerEric
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Re: Time to shake? [Re: A.k.a]
    #26922656 - 09/07/20 08:49 AM (3 years, 4 months ago)

Quote:

A.k.a said:
Much better. Keep an eye on it though, sometimes mold won’t show up until like 10 days in or more.

Some spots still look sketchy but that’s at least actual myc in there.


Actually after looking closer I think the second pic has mold underneath the top myc sprout. The whole middle area where there’s growth looks like similar mold to the first jars in the op.

How do your plates look? Idk where the syringes came from but starting to seem likely they’re responsible. If the plates grow the same shit I’d try to get a refund.





Syringes are from a vendor from a different site. Plates were done via spore prints & swabs.

Here's the plates in order: Maz, wild gt, & mammoth gt. First time doing any of this so yes, lots of spore on the plates. Growth originated from a spore clump on the wild gt plate.




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OfflineFarmerEric
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Re: Time to shake? [Re: A.k.a]
    #26922658 - 09/07/20 08:51 AM (3 years, 4 months ago)

Quote:

A.k.a said:
No spores are usually carrying contamination mixed in with them. Certain vendors use machines that remove most of it but I only know of one place that does that for sure.

I’d guess the syringes are loaded with spores from that mold. Moisture won’t make contams materialize in the jar but it will make it easier for them to grow if they’re already in there.




My plan is for agar and g2g I only tried these syringes bc I had them left over and thought why not. They worked in April but I messed up the shoebox and it got contam (opened way too often).


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Re: Time to shake? [Re: FarmerEric]
    #26922681 - 09/07/20 09:11 AM (3 years, 4 months ago)

Quote:

FarmerEric said:
got contam (opened way too often).



That's not why you got contams...it's was your spawn and from what I'm gathering it is indeed the syringe or your sterile practice or both. Try the syringe again on agar and work for a clean culture.


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OfflineFarmerEric
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Re: Time to shake? [Re: Tormato]
    #26922692 - 09/07/20 09:16 AM (3 years, 4 months ago)

Quote:

Tormato said:
Quote:

FarmerEric said:
got contam (opened way too often).



That's not why you got contams...it's was your spawn and from what I'm gathering it is indeed the syringe or your sterile practice or both. Try the syringe again on agar and work for a clean culture.




Makes sense the syringe would be to blame. I had a 3lb bag of oats that went green mold and I thought it was the grain. The bag was a different syringe than the clearly bad jars but both syringes from the same seller.

To use a syringe onnagar I'd aim for just a couple drops of water in the middle correct?

Thank you all for the help and tough love. It sucks to hear these are lost but I'm glad to learn from this to avoid it in the future!

Side question: how long do grain jars remain "good" on average after pc, a week?


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Re: Time to shake? [Re: FarmerEric]
    #26922702 - 09/07/20 09:26 AM (3 years, 4 months ago)

With the syringe try to get one drop on the plate if you can, as little as possible.

Prepped jars will be fine after a week, and even longer depending on how much moisture they’re losing.


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Re: Time to shake? [Re: FarmerEric]
    #26922704 - 09/07/20 09:28 AM (3 years, 4 months ago)

One drop is plenty.

If I don't use my sterilized grains within a week I start over...some have gone longer, but I worry more about the hydration of the grains versus contams when letting them sit unused for awhile.

What A.k.a. said too.


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OfflineFarmerEric
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Re: Time to shake? [Re: FarmerEric]
    #26922715 - 09/07/20 09:33 AM (3 years, 4 months ago)

Quote:

FarmerEric said:
Quote:

A.k.a said:
Much better. Keep an eye on it though, sometimes mold won’t show up until like 10 days in or more.

Some spots still look sketchy but that’s at least actual myc in there.


Actually after looking closer I think the second pic has mold underneath the top myc sprout. The whole middle area where there’s growth looks like similar mold to the first jars in the op.

How do your plates look? Idk where the syringes came from but starting to seem likely they’re responsible. If the plates grow the same shit I’d try to get a refund.





Syringes are from a vendor from a different site. Plates were done via spore prints & swabs.

Here's the plates in order: Maz, wild gt, & mammoth gt. First time doing any of this so yes, lots of spore on the plates. Growth originated from a spore clump on the wild gt plate.








Any wisdom for my plates? They're supposed to be wrapped in parafilm during incubation right?

And since this one is near the edge should I isolate and move to a new plate?




Edited by FarmerEric (09/07/20 09:47 AM)


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Re: Time to shake? [Re: FarmerEric]
    #26922726 - 09/07/20 09:43 AM (3 years, 4 months ago)

Quote:

FarmerEric said:




Any wisdom for my plates? They're supposed to be wrapped in parafilm during incubation right?

And since this one is near the edge should I isolate and move to a new plate?





That is mold bro.

Yes wrap with Parafilm or Clingwrap works too.


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Re: Time to shake? [Re: Tormato]
    #26922733 - 09/07/20 09:48 AM (3 years, 4 months ago)

It’s all the same mold too which is weird.

Maybe the place you got your spores had a big outbreak of it that contaminated a lot of their stuff.  If it was your sterile technique I think there would be more satellite colonies on there.


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Edited by A.k.a (09/07/20 09:49 AM)


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OfflineFarmerEric
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Re: Time to shake? [Re: A.k.a]
    #26922846 - 09/07/20 11:19 AM (3 years, 4 months ago)

Quote:

A.k.a said:
It’s all the same mold too which is weird.

Maybe the place you got your spores had a big outbreak of it that contaminated a lot of their stuff.  If it was your sterile technique I think there would be more satellite colonies on there.




It's so damn strange bc the growth started on a spot with spores. The prints, swabs, and syringes all from different sources. WBS was cooked for 90min @15psi that should kill any spores right? I'm so confused too and a little deflated.



Do all of my plates look like mold or just the wild gt? And being wild, could that be the cause of the mold?


Edited by FarmerEric (09/07/20 11:22 AM)


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OfflineFarmerEric
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Re: Time to shake? [Re: FarmerEric]
    #26922912 - 09/07/20 12:02 PM (3 years, 4 months ago)

Also, I keep the plates and jars around 75-78 Fahrenheit  is that OK?


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Re: Time to shake? [Re: FarmerEric]
    #26922918 - 09/07/20 12:03 PM (3 years, 4 months ago)

Did you vent the pc and not start the timer until it was at 15psi?

If the spores are all from different places then the molds probably on your end. Even if it’s where the spores are it’s just mixed in and grows quicker than cube spores.


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Re: Time to shake? [Re: A.k.a]
    #26922980 - 09/07/20 12:36 PM (3 years, 4 months ago)

The green areas actually look like cube myc to me, I'd transfer them and see what happens


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OfflineFarmerEric
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Re: Time to shake? [Re: A.k.a]
    #26922981 - 09/07/20 12:36 PM (3 years, 4 months ago)

Quote:

Did you vent the pc and not start the timer until it was at 15psi?

If the spores are all from different places then the molds probably on your end. Even if it’s where the spores are it’s just mixed in and grows quicker than cube spores.






I have an AA so I purged it for the first 5 Mins of steam as directed then closed the valve, back to pressure (15psi 250 degrees) and purged again for 5 minutes as directed, closed it and brought it back to pressure then began the timer.


Edited by FarmerEric (09/07/20 12:38 PM)


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OfflineFarmerEric
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Re: Time to shake? [Re: sporecap]
    #26922985 - 09/07/20 12:37 PM (3 years, 4 months ago)

Quote:

sporecap said:
The green areas actually look like cube myc to me, I'd transfer them and see what happens





Will do as I have a few plates. BUT are there any extra precautions to take? And can I do it now?


Edited by FarmerEric (09/07/20 12:39 PM)


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Re: Time to shake? [Re: FarmerEric]
    #26922989 - 09/07/20 12:39 PM (3 years, 4 months ago)

So far it seems to me that the mold you see is coming from the syringe, since
it's also growing on your plates. You can do a control plate, just leave it empty but wrap it and store it like the others you used for your transfers. If this one stays clean, you know your technique is good.


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Re: Time to shake? [Re: sporecap]
    #26923017 - 09/07/20 12:54 PM (3 years, 4 months ago)

Quote:

sporecap said:
So far it seems to me that the mold you see is coming from the syringe, since
it's also growing on your plates. You can do a control plate, just leave it empty but wrap it and store it like the others you used for your transfers. If this one stays clean, you know your technique is good.



Plates are not from syringes. Jars are from syringe.

When you say "leave it empty" for the plate do you mean unwrap, open it in the SAB and then seal not as if I actually did inoculate?


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Re: Time to shake? [Re: FarmerEric]
    #26923268 - 09/07/20 03:16 PM (3 years, 4 months ago)

Yes, take a Petri dish with agar in the SAB, open it, do all the movements you would do when inocculating it.
But don't put any spores in. Then seal as normal and store with your other plates.
If your technique is steril, this plate will stay clean with no growth.
If it contams, you know your technique is the problem (high sporeload in the air, too much movemnts in the SAB, agar not properly sterilized, etc)


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